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Comparison of cytomegalovirus-specific immune cell response to proteins versus peptides using an IFN-γ ELISpot assay after hematopoietic stem cell transplantation

Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral...

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Hauptverfasser: Wagner-Drouet, Eva (VerfasserIn) , Teschner, Daniel (VerfasserIn) , Wolschke, Christine (VerfasserIn) , Schäfer-Eckart, Kerstin (VerfasserIn) , Gärtner, Johannes (VerfasserIn) , Mielke, Stephan (VerfasserIn) , Schreder, Martin (VerfasserIn) , Kobbe, Guido (VerfasserIn) , Hilgendorf, Inken (VerfasserIn) , Klein, Stefan (VerfasserIn) , Verbeek, Mareike (VerfasserIn) , Ditschkowski, Markus (VerfasserIn) , Koch, Martina (VerfasserIn) , Lindemann, Monika (VerfasserIn) , Schmidt, Traudel (VerfasserIn) , Rascle, Anne (VerfasserIn) , Barabas, Sascha (VerfasserIn) , Deml, Ludwig (VerfasserIn) , Wagner, Ralf (VerfasserIn) , Wolff, Daniel (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 5 February 2021
In: Diagnostics
Year: 2021, Jahrgang: 11, Heft: 2, Pages: 1-13
ISSN:2075-4418
DOI:10.3390/diagnostics11020312
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/diagnostics11020312
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2075-4418/11/2/312
Volltext
Verfasserangaben:Eva Wagner-Drouet, Daniel Teschner, Christine Wolschke, Kerstin Schäfer-Eckart, Johannes Gärtner, Stephan Mielke, Martin Schreder, Guido Kobbe, Inken Hilgendorf, Stefan Klein, Mareike Verbeek, Markus Ditschkowski, Martina Koch, Monika Lindemann, Traudel Schmidt, Anne Rascle, Sascha Barabas, Ludwig Deml, Ralf Wagner and Daniel Wolff
Beschreibung
Zusammenfassung:Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-γ ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-γ ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8+ counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
Beschreibung:Gesehen am 07.07.2021
Beschreibung:Online Resource
ISSN:2075-4418
DOI:10.3390/diagnostics11020312

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