Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration
Cutaneous regeneration utilizes paracrine feedback mechanisms to fine-tune the regulation of epidermal keratinocyte proliferation and migration. However, it is unknown how fibroblast-derived hepatocyte growth factor (HGF) affects these mutually exclusive processes in distinct cell populations. We he...
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| Hauptverfasser: | , , , , , , , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
September 16, 2013
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| In: |
PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 9, Pages: 1-15 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0075075 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0075075 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075075 
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| Verfasserangaben: | Sabrina Schmitt, Kai Safferling, Kathi Westphal, Manuel Hrabowski, Ute Müller, Peter Angel, Lars Wiechert, Volker Ehemann, Benedikt Müller, Stefan Holland-Cunz, Damian Stichel, Nathalie Harder, Karl Rohr, Günter Germann, Franziska Matthäus, Peter Schirmacher, Niels Grabe, Kai Breuhahn |
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| 245 | 1 | 0 | |a Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration |c Sabrina Schmitt, Kai Safferling, Kathi Westphal, Manuel Hrabowski, Ute Müller, Peter Angel, Lars Wiechert, Volker Ehemann, Benedikt Müller, Stefan Holland-Cunz, Damian Stichel, Nathalie Harder, Karl Rohr, Günter Germann, Franziska Matthäus, Peter Schirmacher, Niels Grabe, Kai Breuhahn |
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| 520 | |a Cutaneous regeneration utilizes paracrine feedback mechanisms to fine-tune the regulation of epidermal keratinocyte proliferation and migration. However, it is unknown how fibroblast-derived hepatocyte growth factor (HGF) affects these mutually exclusive processes in distinct cell populations. We here show that HGF stimulates the expression and phosphorylation of the microtubule-destabilizing factor stathmin in primary human keratinocytes. Quantitative single cell- and cell population-based analyses revealed that basal stathmin levels are important for the migratory ability of keratinocytes in vitro; however, its expression is moderately induced in the migration tongue of mouse skin or organotypic multi-layered keratinocyte 3D cultures after full-thickness wounding. In contrast, clearly elevated stathmin expression is detectable in hyperproliferative epidermal areas. In vitro, stathmin silencing significantly reduced keratinocyte proliferation. Automated quantitative and time-resolved analyses in organotypic cocultures demonstrated a high correlation between Stathmin/phospho-Stathmin and Ki67 positivity in epidermal regions with proliferative activity. Thus, activation of stathmin may stimulate keratinocyte proliferation, while basal stathmin levels are sufficient for keratinocyte migration during cutaneous regeneration. | ||
| 650 | 4 | |a Cell cycle and cell division | |
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| 650 | 4 | |a Growth factors | |
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| 650 | 4 | |a Phosphorylation | |
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| 650 | 4 | |a Wound healing | |
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