Grazing-incidence neutron-induced fluorescence probes density profiles of labeled molecules at solid/liquid interfaces
We report on the use of characteristic prompt γ-fluorescence after neutron capture induced by an evanescent neutron wave to probe densities and depth profiles of labeled molecules at solid/liquid interfaces. In contrast to classical scattering techniques and X-ray fluorescence, this method of “grazi...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 5, 2013
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| In: |
Langmuir
Year: 2013, Volume: 29, Issue: 12, Pages: 4084-4091 |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la400162y |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/la400162y
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| Author Notes: | Emanuel Schneck, Michael Jentschel, Christian Gege, Motomu Tanaka, and Bruno Demé |
| Summary: | We report on the use of characteristic prompt γ-fluorescence after neutron capture induced by an evanescent neutron wave to probe densities and depth profiles of labeled molecules at solid/liquid interfaces. In contrast to classical scattering techniques and X-ray fluorescence, this method of “grazing-incidence neutron-induced fluorescence” combines direct chemical specificity, provided by the label, with sensitivity to the interface, inherent to the evanescent wave. We demonstrate that the formation of a supported lipid membrane can be quantitatively monitored from the characteristic fluorescence of 157Gd3+ ions bound to the headgroup of chelator lipids. Moreover, we were able to localize the 157Gd3+ ions along the surface normal with nanometer precision. This first proof of principle with a well-defined model system suggests that the method has a great potential for biology and soft matter studies where spatial resolution and chemical sensitivity are required. |
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| Item Description: | Gesehen am 12.07.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la400162y |