Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels-Alder bioconjugation
Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Ald...
Gespeichert in:
| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
28 Jan 2013
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| In: |
RSC Advances
Year: 2013, Jahrgang: 3, Heft: 13, Pages: 4181-4183 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/C3RA40373D |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C3RA40373D Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra40373d 
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| Verfasserangaben: | Juliane Schoch and Andres Jäschke |
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| 520 | |a Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Alder reaction. | ||
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