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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels-Alder bioconjugation

Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Ald...

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Hauptverfasser: Schoch, Juliane (VerfasserIn) , Jäschke, Andres (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 28 Jan 2013
In: RSC Advances
Year: 2013, Jahrgang: 3, Heft: 13, Pages: 4181-4183
ISSN:2046-2069
DOI:10.1039/C3RA40373D
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C3RA40373D
Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra40373d
Volltext
Verfasserangaben:Juliane Schoch and Andres Jäschke
Beschreibung
Zusammenfassung:Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Alder reaction.
Beschreibung:Gesehen am 15.07.2021
Beschreibung:Online Resource
ISSN:2046-2069
DOI:10.1039/C3RA40373D

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