HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer
Heightened expression of human endogenous retrovirus (HERV) sequences has been associated with a range of malignancies, including prostate cancer, suggesting that they may serve as useful diagnostic or prognostic cancer biomarkers. We analysed the expression of HERV-K (Gag and Env/Np9 regions), HERV...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
10 March 2021
|
| In: |
Viruses
Year: 2021, Jahrgang: 13, Heft: 3, Pages: 1-12 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13030449 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/v13030449 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1999-4915/13/3/449 
|
| Verfasserangaben: | Simin D. Rezaei, Joshua A. Hayward, Sam Norden, John Pedersen, John Mills, Anna C. Hearps and Gilda Tachedjian |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1765921163 | ||
| 003 | DE-627 | ||
| 005 | 20220820031906.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210808s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/v13030449 |2 doi | |
| 035 | |a (DE-627)1765921163 | ||
| 035 | |a (DE-599)KXP1765921163 | ||
| 035 | |a (OCoLC)1341420392 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Rezaei, Simin Dokht |e VerfasserIn |0 (DE-588)1238499775 |0 (DE-627)1765921147 |4 aut | |
| 245 | 1 | 0 | |a HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer |c Simin D. Rezaei, Joshua A. Hayward, Sam Norden, John Pedersen, John Mills, Anna C. Hearps and Gilda Tachedjian |
| 264 | 1 | |c 10 March 2021 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.08.2021 | ||
| 520 | |a Heightened expression of human endogenous retrovirus (HERV) sequences has been associated with a range of malignancies, including prostate cancer, suggesting that they may serve as useful diagnostic or prognostic cancer biomarkers. We analysed the expression of HERV-K (Gag and Env/Np9 regions), HERV-E 4.1 (Pol and Env regions), HERV-H (Pol) and HERV-W (Gag) sequences in prostate cancer cells lines and normal prostate epithelial cells using qRT-PCR. HERV expression was also analysed in matched malignant and benign prostate tissue samples from men with prostate cancer (n = 27, median age 65.2 years (range 47-70)) and compared to prostate cancer-free male controls (n = 11). Prostate cancer epithelial cell lines exhibited a signature of HERV RNA overexpression, with all HERVs analysed, except HERV-E Pol, showing heightened expression in at least two, but more commonly all, cell lines analysed. Analysis of primary prostate material indicated increased expression of HERV-E Pol but decreased expression of HERV-E Env in both malignant and benign regions of the prostate in men with prostate cancer as compared to those without. Expression of HERV-K Gag was significantly higher in malignant regions of the prostate in men with prostate cancer as compared to matched benign regions and prostate cancer-free men (p < 0.001 for both), with 85.2% of prostate cancers donors showing malignancy-associated upregulation of HERV-K Gag RNA. HERV-K Gag protein was detected in 12/18 (66.7%) malignant tissues using immunohistochemistry, but only 1/18 (5.6%) benign tissue sections. Heightened expression of HERV-K Gag RNA and protein appears to be a sensitive and specific biomarker of prostate malignancy in this cohort of men with prostate carcinoma, supporting its potential utility as a non-invasive, adjunct clinical biomarker. | ||
| 650 | 4 | |a HERV-K | |
| 650 | 4 | |a human endogenous retrovirus (HERV) | |
| 650 | 4 | |a prostate cancer | |
| 700 | 1 | |a Hayward, Joshua A. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Norden, Sam |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pedersen, John |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mills, John |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hearps, Anna C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tachedjian, Gilda |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Viruses |d Basel : MDPI, 2009 |g 13(2021), 3 vom: März, Artikel-ID 449, Seite 1-12 |h Online-Ressource |w (DE-627)609775871 |w (DE-600)2516098-9 |w (DE-576)311326080 |x 1999-4915 |7 nnas |a HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer |
| 773 | 1 | 8 | |g volume:13 |g year:2021 |g number:3 |g month:03 |g elocationid:449 |g pages:1-12 |g extent:12 |a HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer |
| 856 | 4 | 0 | |u https://doi.org/10.3390/v13030449 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/1999-4915/13/3/449 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210808 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1238499775 |a Rezaei, Simin Dokht |m 1238499775:Rezaei, Simin Dokht |p 1 |x j | ||
| 999 | |a KXP-PPN1765921163 |e 3963603526 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 08.08.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"recId":"1765921163","person":[{"role":"aut","display":"Rezaei, Simin Dokht","roleDisplay":"VerfasserIn","given":"Simin Dokht","family":"Rezaei"},{"role":"aut","display":"Hayward, Joshua A.","roleDisplay":"VerfasserIn","given":"Joshua A.","family":"Hayward"},{"roleDisplay":"VerfasserIn","display":"Norden, Sam","role":"aut","family":"Norden","given":"Sam"},{"given":"John","family":"Pedersen","role":"aut","roleDisplay":"VerfasserIn","display":"Pedersen, John"},{"role":"aut","display":"Mills, John","roleDisplay":"VerfasserIn","given":"John","family":"Mills"},{"display":"Hearps, Anna C.","roleDisplay":"VerfasserIn","role":"aut","family":"Hearps","given":"Anna C."},{"given":"Gilda","family":"Tachedjian","role":"aut","display":"Tachedjian, Gilda","roleDisplay":"VerfasserIn"}],"title":[{"title_sort":"HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer","title":"HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancer"}],"physDesc":[{"extent":"12 S."}],"relHost":[{"title":[{"title":"Viruses","title_sort":"Viruses"}],"pubHistory":["1.2009 -"],"part":{"issue":"3","pages":"1-12","year":"2021","extent":"12","volume":"13","text":"13(2021), 3 vom: März, Artikel-ID 449, Seite 1-12"},"disp":"HERV-K Gag RNA and protein levels are elevated in malignant regions of the prostate in males with prostate cancerViruses","type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"recId":"609775871","origin":[{"publisherPlace":"Basel","dateIssuedKey":"2009","publisher":"MDPI","dateIssuedDisp":"2009-"}],"id":{"issn":["1999-4915"],"eki":["609775871"],"zdb":["2516098-9"]},"name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"physDesc":[{"extent":"Online-Ressource"}]}],"name":{"displayForm":["Simin D. Rezaei, Joshua A. Hayward, Sam Norden, John Pedersen, John Mills, Anna C. Hearps and Gilda Tachedjian"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"10 March 2021"}],"id":{"doi":["10.3390/v13030449"],"eki":["1765921163"]}} | ||
| SRT | |a REZAEISIMIHERVKGAGRN1020 | ||