The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain
Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome in a process requiring the RNA helicase Mtr4 and specific adaptor complexes for RNA substrate recognition. The PAXT and MTREC complexes have recently been identified as homologous exosome adaptors in human and fission ye...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
08 June 2021
|
| In: |
Nature Communications
Year: 2021, Jahrgang: 12, Pages: 1-13 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-021-23565-3 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-021-23565-3 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-021-23565-3 
|
| Verfasserangaben: | Nikolay Dobrev, Yasar Luqman Ahmed, Anusree Sivadas, Komal Soni, Tamás Fischer & Irmgard Sinning |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1767083602 | ||
| 003 | DE-627 | ||
| 005 | 20230317091727.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210816s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-021-23565-3 |2 doi | |
| 035 | |a (DE-627)1767083602 | ||
| 035 | |a (DE-599)KXP1767083602 | ||
| 035 | |a (OCoLC)1341420657 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Dobrev, Nikolay |d 1987- |e VerfasserIn |0 (DE-588)1213118263 |0 (DE-627)1703443861 |4 aut | |
| 245 | 1 | 4 | |a The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain |c Nikolay Dobrev, Yasar Luqman Ahmed, Anusree Sivadas, Komal Soni, Tamás Fischer & Irmgard Sinning |
| 264 | 1 | |c 08 June 2021 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.08.2021 | ||
| 520 | |a Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome in a process requiring the RNA helicase Mtr4 and specific adaptor complexes for RNA substrate recognition. The PAXT and MTREC complexes have recently been identified as homologous exosome adaptors in human and fission yeast, respectively. The eleven-subunit MTREC comprises the zinc-finger protein Red1 and the Mtr4 homologue Mtl1. Here, we use yeast two-hybrid and pull-down assays to derive a detailed interaction map. We show that Red1 bridges MTREC submodules and serves as the central scaffold. In the crystal structure of a minimal Mtl1/Red1 complex an unstructured region adjacent to the Red1 zinc-finger domain binds to both the Mtl1 KOW domain and stalk helices. This interaction extends the canonical interface seen in Mtr4-adaptor complexes. In vivo mutational analysis shows that this interface is essential for cell survival. Our results add to Mtr4 versatility and provide mechanistic insights into the MTREC complex. | ||
| 700 | 1 | |a Ahmed, Yasar Luqman |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sivadas, Anusree |e VerfasserIn |4 aut | |
| 700 | 1 | |a Soni, Komal |e VerfasserIn |0 (DE-588)1197176748 |0 (DE-627)1678987417 |4 aut | |
| 700 | 1 | |a Fischer, Tamás |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sinning, Irmgard |d 1960- |e VerfasserIn |0 (DE-588)1027598617 |0 (DE-627)72945133X |0 (DE-576)166290580 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 12(2021), Artikel-ID 3456, Seite 1-13 |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain |
| 773 | 1 | 8 | |g volume:12 |g year:2021 |g elocationid:3456 |g pages:1-13 |g extent:13 |a The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-021-23565-3 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-021-23565-3 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210816 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1027598617 |a Sinning, Irmgard |m 1027598617:Sinning, Irmgard |d 700000 |d 711000 |e 700000PS1027598617 |e 711000PS1027598617 |k 0/700000/ |k 1/700000/711000/ |p 6 |y j | ||
| 998 | |g 1197176748 |a Soni, Komal |m 1197176748:Soni, Komal |d 700000 |d 711000 |e 700000PS1197176748 |e 711000PS1197176748 |k 0/700000/ |k 1/700000/711000/ |p 4 | ||
| 999 | |a KXP-PPN1767083602 |e 3969238005 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"disp":"The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domainNature Communications","recId":"626457688","language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"pubHistory":["2010-"],"origin":[{"publisher":"Springer Nature ; Nature Publishing Group UK","dateIssuedDisp":"[2010]-","publisherPlace":"[London] ; [London]"}],"title":[{"title":"Nature Communications","title_sort":"Nature Communications"}],"part":{"volume":"12","text":"12(2021), Artikel-ID 3456, Seite 1-13","extent":"13","year":"2021","pages":"1-13"},"id":{"eki":["626457688"],"issn":["2041-1723"],"zdb":["2553671-0"]},"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 13.06.24"]}],"note":["Gesehen am 16.08.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"eki":["1767083602"],"doi":["10.1038/s41467-021-23565-3"]},"physDesc":[{"extent":"13 S."}],"language":["eng"],"title":[{"title":"The zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain","title_sort":"zinc-finger protein Red1 orchestrates MTREC submodules and binds the Mtl1 helicase arch domain"}],"recId":"1767083602","name":{"displayForm":["Nikolay Dobrev, Yasar Luqman Ahmed, Anusree Sivadas, Komal Soni, Tamás Fischer & Irmgard Sinning"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"08 June 2021"}],"person":[{"given":"Nikolay","display":"Dobrev, Nikolay","family":"Dobrev","role":"aut"},{"given":"Yasar Luqman","display":"Ahmed, Yasar Luqman","family":"Ahmed","role":"aut"},{"role":"aut","given":"Anusree","display":"Sivadas, Anusree","family":"Sivadas"},{"role":"aut","family":"Soni","given":"Komal","display":"Soni, Komal"},{"role":"aut","family":"Fischer","display":"Fischer, Tamás","given":"Tamás"},{"family":"Sinning","given":"Irmgard","display":"Sinning, Irmgard","role":"aut"}]} | ||
| SRT | |a DOBREVNIKOZINCFINGER0820 | ||