RhoGEF17: an essential regulator of endothelial cell death and growth
The Rho guanine nucleotide exchange factor RhoGEF17 was described to reside in adherens junctions (AJ) in endothelial cells (EC) and to play a critical role in the regulation of cell adhesion and barrier function. The purpose of this study was to analyze signal cascades and processes occurring subse...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
27 March 2021
|
| In: |
Cells
Year: 2021, Jahrgang: 10, Heft: 4, Pages: 1-17 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10040741 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cells10040741 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2073-4409/10/4/741 
|
| Verfasserangaben: | Pamina Weber, Doris Baltus, Aline Jatho, Oliver Drews, Laura C. Zelarayan, Thomas Wieland and Susanne Lutz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1767098332 | ||
| 003 | DE-627 | ||
| 005 | 20230426082438.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210816s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cells10040741 |2 doi | |
| 035 | |a (DE-627)1767098332 | ||
| 035 | |a (DE-599)KXP1767098332 | ||
| 035 | |a (OCoLC)1341420666 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Weber, Pamina |d 1984- |e VerfasserIn |0 (DE-588)1033580864 |0 (DE-627)741720701 |0 (DE-576)381207463 |4 aut | |
| 245 | 1 | 0 | |a RhoGEF17 |b an essential regulator of endothelial cell death and growth |c Pamina Weber, Doris Baltus, Aline Jatho, Oliver Drews, Laura C. Zelarayan, Thomas Wieland and Susanne Lutz |
| 264 | 1 | |c 27 March 2021 | |
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 16.08.2021 | ||
| 520 | |a The Rho guanine nucleotide exchange factor RhoGEF17 was described to reside in adherens junctions (AJ) in endothelial cells (EC) and to play a critical role in the regulation of cell adhesion and barrier function. The purpose of this study was to analyze signal cascades and processes occurring subsequent to AJ disruption induced by RhoGEF17 knockdown. Primary human and immortalized rat EC were used to demonstrate that an adenoviral-mediated knockdown of RhoGEF17 resulted in cell rounding and an impairment in spheroid formation due to an enhanced proteasomal degradation of AJ components. In contrast, β-catenin degradation was impaired, which resulted in an induction of the β-catenin-target genes cyclin D1 and survivin. RhoGEF17 depletion additionally inhibited cell adhesion and sheet migration. The RhoGEF17 knockdown prevented the cells with impeded cell-cell and cell-matrix contacts from apoptosis, which was in line with a reduction in pro-caspase 3 expression and an increase in Akt phosphorylation. Nevertheless, the cells were not able to proliferate as a cell cycle block occurred. In summary, we demonstrate that a loss of RhoGEF17 disturbs cell-cell and cell-substrate interaction in EC. Moreover, it prevents the EC from cell death and blocks cell proliferation. Non-canonical β-catenin signaling and Akt activation could be identified as a potential mechanism. | ||
| 650 | 4 | |a adherens junction complex | |
| 650 | 4 | |a anchorage-dependent cell death | |
| 650 | 4 | |a endothelial cell | |
| 650 | 4 | |a non-canonical β-catenin signaling | |
| 650 | 4 | |a rho guanine nucleotide exchange factor | |
| 700 | 1 | |a Baltus, Doris |d 1960- |e VerfasserIn |0 (DE-588)1239068530 |0 (DE-627)1767097972 |4 aut | |
| 700 | 1 | |a Jatho, Aline |e VerfasserIn |4 aut | |
| 700 | 1 | |a Drews, Oliver |d 1974- |e VerfasserIn |0 (DE-588)129902098 |0 (DE-627)483349453 |0 (DE-576)297894587 |4 aut | |
| 700 | 1 | |a Zelarayan, Laura C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wieland, Thomas |d 1960-2025 |e VerfasserIn |0 (DE-588)1033445908 |0 (DE-627)741233215 |0 (DE-576)381043339 |4 aut | |
| 700 | 1 | |a Lutz, Susanne |d 1969- |e VerfasserIn |0 (DE-588)1229012338 |0 (DE-627)1750947242 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cells |d Basel : MDPI, 2012 |g 10(2021), 4, Artikel-ID 741, Seite 1-17 |h Online-Ressource |w (DE-627)718622081 |w (DE-600)2661518-6 |w (DE-576)365412112 |x 2073-4409 |7 nnas |a RhoGEF17 an essential regulator of endothelial cell death and growth |
| 773 | 1 | 8 | |g volume:10 |g year:2021 |g number:4 |g elocationid:741 |g pages:1-17 |g extent:17 |a RhoGEF17 an essential regulator of endothelial cell death and growth |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cells10040741 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2073-4409/10/4/741 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210816 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1229012338 |a Lutz, Susanne |m 1229012338:Lutz, Susanne |d 60000 |e 60000PL1229012338 |k 0/60000/ |p 7 |y j | ||
| 998 | |g 1033445908 |a Wieland, Thomas |m 1033445908:Wieland, Thomas |d 60000 |d 65000 |e 60000PW1033445908 |e 65000PW1033445908 |k 0/60000/ |k 1/60000/65000/ |p 6 | ||
| 998 | |g 129902098 |a Drews, Oliver |m 129902098:Drews, Oliver |d 140000 |e 140000PD129902098 |k 0/140000/ |p 4 | ||
| 998 | |g 1239068530 |a Baltus, Doris |m 1239068530:Baltus, Doris |d 60000 |d 65000 |e 60000PB1239068530 |e 65000PB1239068530 |k 0/60000/ |k 1/60000/65000/ |p 2 | ||
| 998 | |g 1033580864 |a Weber, Pamina |m 1033580864:Weber, Pamina |d 60000 |d 65000 |e 60000PW1033580864 |e 65000PW1033580864 |k 0/60000/ |k 1/60000/65000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1767098332 |e 3969315344 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"17 S."}],"note":["Gesehen am 16.08.2021"],"name":{"displayForm":["Pamina Weber, Doris Baltus, Aline Jatho, Oliver Drews, Laura C. Zelarayan, Thomas Wieland and Susanne Lutz"]},"person":[{"roleDisplay":"VerfasserIn","display":"Weber, Pamina","role":"aut","given":"Pamina","family":"Weber"},{"given":"Doris","family":"Baltus","roleDisplay":"VerfasserIn","display":"Baltus, Doris","role":"aut"},{"given":"Aline","family":"Jatho","roleDisplay":"VerfasserIn","display":"Jatho, Aline","role":"aut"},{"given":"Oliver","family":"Drews","role":"aut","display":"Drews, Oliver","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Zelarayan, Laura C.","role":"aut","family":"Zelarayan","given":"Laura C."},{"role":"aut","display":"Wieland, Thomas","roleDisplay":"VerfasserIn","given":"Thomas","family":"Wieland"},{"roleDisplay":"VerfasserIn","role":"aut","display":"Lutz, Susanne","family":"Lutz","given":"Susanne"}],"id":{"eki":["1767098332"],"doi":["10.3390/cells10040741"]},"recId":"1767098332","type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"subtitle":"an essential regulator of endothelial cell death and growth","title":"RhoGEF17","title_sort":"RhoGEF17"}],"origin":[{"dateIssuedDisp":"27 March 2021","dateIssuedKey":"2021"}],"relHost":[{"title":[{"subtitle":"open access journal","title":"Cells","title_sort":"Cells"}],"origin":[{"publisher":"MDPI","dateIssuedKey":"2012","publisherPlace":"Basel","dateIssuedDisp":"2012-"}],"part":{"issue":"4","year":"2021","volume":"10","extent":"17","pages":"1-17","text":"10(2021), 4, Artikel-ID 741, Seite 1-17"},"disp":"RhoGEF17 an essential regulator of endothelial cell death and growthCells","language":["eng"],"type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"eki":["718622081"],"issn":["2073-4409"],"zdb":["2661518-6"]},"recId":"718622081","physDesc":[{"extent":"Online-Ressource"}],"pubHistory":["1.2012 -"],"note":["Gesehen am 09.05.12"]}],"language":["eng"]} | ||
| SRT | |a WEBERPAMINRHOGEF172720 | ||