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A systems-oriented analysis of the grapevine R2R3-MYB transcription factor family uncovers new insights into the regulation of stilbene accumulation

R2R3-MYB transcription factors (TFs) belong to a large and functionally diverse protein superfamily in plants. In this study, we explore the evolution and function of this family in grapevine (Vitis vinifera L.), a high-value fruit crop. We identified and manually curated 134 genes using RNA-Seq dat...

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Hauptverfasser: Wong, Darren Chern Jan (VerfasserIn) , Höll, Janine (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 12 July 2016
In: DNA research
Year: 2016, Jahrgang: 23, Heft: 5, Pages: 451-466
ISSN:1756-1663
DOI:10.1093/dnares/dsw028
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/dnares/dsw028
Volltext
Verfasserangaben:Darren Chern Jan Wong, Rudolf Schlechter, Alessandro Vannozzi, Janine Höll, Ibrahim Hmmam, Jochen Bogs, Giovanni Battista Tornielli, Simone Diego Castellarin, José Tomás Matus
Beschreibung
Zusammenfassung:R2R3-MYB transcription factors (TFs) belong to a large and functionally diverse protein superfamily in plants. In this study, we explore the evolution and function of this family in grapevine (Vitis vinifera L.), a high-value fruit crop. We identified and manually curated 134 genes using RNA-Seq data, and named them systematically according to the Super-Nomenclature Committee. We identified novel genes, splicing variants and grapevine/woody-specific duplicated subgroups, suggesting possible neo- and sub-functionalization events. Regulatory network analysis ascribed biological functions to uncharacterized genes and validated those of known genes (e.g. secondary cell wall biogenesis and flavonoid biosynthesis). A comprehensive analysis of different MYB binding motifs in the promoters of co-expressed genes predicted grape R2R3-MYB binding preferences and supported evidence for putative downstream targets. Enrichment of cis-regulatory motifs for diverse TFs reinforced the notion of transcriptional coordination and interaction between MYBs and other regulators. Analysis of the network of Subgroup 2 showed that the resveratrol-related VviMYB14 and VviMYB15 share common co-expressed STILBENE SYNTHASE genes with the uncharacterized VviMYB13. These regulators have distinct expression patterns within organs and in response to biotic and abiotic stresses, suggesting a pivotal role of VviMYB13 in regulating stilbene accumulation in vegetative tissues and under biotic stress conditions.
Beschreibung:Gesehen am 16.08.2021
Beschreibung:Online Resource
ISSN:1756-1663
DOI:10.1093/dnares/dsw028

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