A fructan exohydrolase from maize degrades both inulin and levan and co-exists with 1-kestotriose in maize
Enzymes with fructan exohydrolase (FEH) activity are present not only in fructan-synthesizing species but also in non-fructan plants. This has led to speculation about their functions in non-fructan species. Here, a cell wall invertase-related Zm-6&1-FEH2 with no “classical” invertase motif was...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
13 May 2021
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| In: |
International journal of molecular sciences
Year: 2021, Jahrgang: 22, Heft: 10, Pages: 1-14 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22105149 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ijms22105149 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1422-0067/22/10/5149 
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| Verfasserangaben: | Silin Wu, Steffen Greiner, Chongjian Ma, Jiaxin Zhong, Xiaojia Huang, Thomas Rausch and Hongbo Zhao |
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| 245 | 1 | 2 | |a A fructan exohydrolase from maize degrades both inulin and levan and co-exists with 1-kestotriose in maize |c Silin Wu, Steffen Greiner, Chongjian Ma, Jiaxin Zhong, Xiaojia Huang, Thomas Rausch and Hongbo Zhao |
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| 520 | |a Enzymes with fructan exohydrolase (FEH) activity are present not only in fructan-synthesizing species but also in non-fructan plants. This has led to speculation about their functions in non-fructan species. Here, a cell wall invertase-related Zm-6&1-FEH2 with no “classical” invertase motif was identified in maize. Following heterologous expression in Pichia pastoris and in Nicotiana benthamiana leaves, the enzyme activity of recombinant Zm-6&1-FEH2 displays substrate specificity with respect to inulin and levan. Subcellular localization showed Zm-6&1-FEH2 exclusively localized in the apoplast, and its expression profile was strongly dependent on plant development and in response to drought and abscisic acid. Furthermore, formation of 1-kestotriose, an oligofructan, was detected in vivo and in vitro and could be hydrolyzed by Zm-6&1-FEH2. In summary, these results support that Zm-6&1-FEH2 enzyme from maize can degrade both inulin-type and levan-type fructans, and the implications of the co-existence of Zm-6&1-FEH2 and 1-kestotriose are discussed. | ||
| 650 | 4 | |a <i>Zea mays</i> | |
| 650 | 4 | |a 1-kestotriose | |
| 650 | 4 | |a fructan exohydrolase | |
| 650 | 4 | |a inulin | |
| 650 | 4 | |a levan | |
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| 700 | 1 | |a Huang, Xiaojia |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Zhao, Hongbo |e VerfasserIn |4 aut | |
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