Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit
Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit (TE) factor during protein...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2021
|
| In: |
Molecular cell
Year: 2021, Jahrgang: 81, Heft: 2, Pages: 304-322,e1-e16 |
| ISSN: | 1097-4164 |
| DOI: | 10.1016/j.molcel.2020.11.037 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.molcel.2020.11.037 Verlag, lizenzpflichtig, Volltext: https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.1016%2Fj.molcel.2020.11.037&DestApp=DOI&SrcAppSID=C6YYap1tn4dp3Ya7FlH&SrcJTitle=MOLECULAR+CELL&DestDOIRegistrantName=Elsevier 
|
| Verfasserangaben: | Matthew L. Kraushar, Ferdinand Krupp, Dermot Harnett, Paul Turko, Mateusz C. Ambrozkiewicz, Thiemo Sprink, Koshi Imami, Manuel Günnigmann, Ulrike Zinnall, Carlos H. Vieira-Vieira, Theres Schaub, Agnieszka Münster-Wandowski, Jörg Bürger, Ekaterina Borisova, Hiroshi Yamamoto, Mladen-Roko Rasin, Uwe Ohler, Dieter Beule, Thorsten Mielke, Victor Tarabykin, Markus Landthaler, Günter Kramer, Imre Vida, Matthias Selbach, and Christian M.T. Spahn |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1770839135 | ||
| 003 | DE-627 | ||
| 005 | 20220820044148.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210916s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.molcel.2020.11.037 |2 doi | |
| 035 | |a (DE-627)1770839135 | ||
| 035 | |a (DE-599)KXP1770839135 | ||
| 035 | |a (OCoLC)1341421309 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kraushar, Matthew L. |e VerfasserIn |0 (DE-588)1241284903 |0 (DE-627)1770841091 |4 aut | |
| 245 | 1 | 0 | |a Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit |c Matthew L. Kraushar, Ferdinand Krupp, Dermot Harnett, Paul Turko, Mateusz C. Ambrozkiewicz, Thiemo Sprink, Koshi Imami, Manuel Günnigmann, Ulrike Zinnall, Carlos H. Vieira-Vieira, Theres Schaub, Agnieszka Münster-Wandowski, Jörg Bürger, Ekaterina Borisova, Hiroshi Yamamoto, Mladen-Roko Rasin, Uwe Ohler, Dieter Beule, Thorsten Mielke, Victor Tarabykin, Markus Landthaler, Günter Kramer, Imre Vida, Matthias Selbach, and Christian M.T. Spahn |
| 264 | 1 | |c 2021 | |
| 300 | |a 36 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published: December 22, 2020 | ||
| 500 | |a Gesehen am 16.09.2021 | ||
| 520 | |a Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit (TE) factor during protein synthesis at nearatomic resolution by cryoelectron microscopy (cryo-EM). Ribosome profiling demonstrated Ebp1-60S binding is highest during start codon initiation and N-terminal peptide elongation, regulating ribosome occupancy of these codons. Membrane-targeting domains emerging from the 60S tunnel, which recruit SRP/Sec61 to the shared binding site, displace Ebp1. Ebp1 is particularly abundant in the early-born neural stem cell (NSC) lineage and regulates neuronal morphology. Ebp1 especially impacts the synthesis of membrane-targeted cell adhesion molecules (CAMs), measured by pulsed stable isotope labeling by amino acids in cell culture (pSILAC)/bioorthogonal noncanonical amino acid tagging (BONCAT) mass spectrometry (MS). Therefore, Ebp1 is a central component of protein synthesis, and the ribosome TE is a focal point of gene expression control in the molecular specification of neuronal morphology during development. | ||
| 650 | 4 | |a binding-protein | |
| 650 | 4 | |a complex | |
| 650 | 4 | |a cryo-em | |
| 650 | 4 | |a ebp1 | |
| 650 | 4 | |a gene-expression | |
| 650 | 4 | |a messenger-rna translation | |
| 650 | 4 | |a platform | |
| 650 | 4 | |a ribosome signature | |
| 650 | 4 | |a stem-cells | |
| 650 | 4 | |a visualization | |
| 700 | 1 | |a Krupp, Ferdinand |e VerfasserIn |4 aut | |
| 700 | 1 | |a Harnett, Dermot |e VerfasserIn |4 aut | |
| 700 | 1 | |a Turko, Paul |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ambrozkiewicz, Mateusz C. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sprink, Thiemo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Imami, Koshi |e VerfasserIn |4 aut | |
| 700 | 1 | |a Günnigmann, Manuel |d 1993- |e VerfasserIn |0 (DE-588)1241284547 |0 (DE-627)1770840397 |4 aut | |
| 700 | 1 | |a Zinnall, Ulrike |e VerfasserIn |4 aut | |
| 700 | 1 | |a Vieira-Vieira, Carlos H. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schaub, Theres |e VerfasserIn |4 aut | |
| 700 | 1 | |a Münster-Wandowski, Agnieszka |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bürger, Jörg |e VerfasserIn |4 aut | |
| 700 | 1 | |a Borisova, Ekaterina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Yamamoto, Hiroshi |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rasin, Mladen-Roko |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ohler, Uwe |e VerfasserIn |4 aut | |
| 700 | 1 | |a Beule, Dieter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mielke, Thorsten |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tarabykin, Victor |e VerfasserIn |4 aut | |
| 700 | 1 | |a Landthaler, Markus |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kramer, Günter |e VerfasserIn |4 aut | |
| 700 | 1 | |a Vida, Imre |e VerfasserIn |4 aut | |
| 700 | 1 | |a Selbach, Matthias |e VerfasserIn |4 aut | |
| 700 | 1 | |a Spahn, Christian M. T. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular cell |d [Cambridge, Mass.] : Cell Press, 1997 |g 81(2021), 2, Seite 304-322,e1-e16 |h Online-Ressource |w (DE-627)320416097 |w (DE-600)2001948-8 |w (DE-576)090881362 |x 1097-4164 |7 nnas |a Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit |
| 773 | 1 | 8 | |g volume:81 |g year:2021 |g number:2 |g pages:304-322,e1-e16 |g extent:36 |a Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.molcel.2020.11.037 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.1016%2Fj.molcel.2020.11.037&DestApp=DOI&SrcAppSID=C6YYap1tn4dp3Ya7FlH&SrcJTitle=MOLECULAR+CELL&DestDOIRegistrantName=Elsevier |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210916 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1241284547 |a Günnigmann, Manuel |m 1241284547:Günnigmann, Manuel |d 140000 |e 140000PG1241284547 |k 0/140000/ |p 8 | ||
| 999 | |a KXP-PPN1770839135 |e 3978939355 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1770839135","language":["eng"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Published: December 22, 2020","Gesehen am 16.09.2021"],"person":[{"family":"Kraushar","given":"Matthew L.","roleDisplay":"VerfasserIn","display":"Kraushar, Matthew L.","role":"aut"},{"family":"Krupp","given":"Ferdinand","display":"Krupp, Ferdinand","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Harnett, Dermot","role":"aut","family":"Harnett","given":"Dermot"},{"display":"Turko, Paul","roleDisplay":"VerfasserIn","role":"aut","family":"Turko","given":"Paul"},{"display":"Ambrozkiewicz, Mateusz C.","roleDisplay":"VerfasserIn","role":"aut","family":"Ambrozkiewicz","given":"Mateusz C."},{"family":"Sprink","given":"Thiemo","display":"Sprink, Thiemo","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Imami","given":"Koshi","display":"Imami, Koshi","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Manuel","family":"Günnigmann","role":"aut","roleDisplay":"VerfasserIn","display":"Günnigmann, Manuel"},{"given":"Ulrike","family":"Zinnall","role":"aut","roleDisplay":"VerfasserIn","display":"Zinnall, Ulrike"},{"family":"Vieira-Vieira","given":"Carlos H.","display":"Vieira-Vieira, Carlos H.","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Schaub","given":"Theres","display":"Schaub, Theres","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Münster-Wandowski, Agnieszka","roleDisplay":"VerfasserIn","given":"Agnieszka","family":"Münster-Wandowski"},{"roleDisplay":"VerfasserIn","display":"Bürger, Jörg","role":"aut","family":"Bürger","given":"Jörg"},{"family":"Borisova","given":"Ekaterina","display":"Borisova, Ekaterina","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Yamamoto","given":"Hiroshi","display":"Yamamoto, Hiroshi","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Rasin, Mladen-Roko","role":"aut","family":"Rasin","given":"Mladen-Roko"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Ohler, Uwe","given":"Uwe","family":"Ohler"},{"family":"Beule","given":"Dieter","roleDisplay":"VerfasserIn","display":"Beule, Dieter","role":"aut"},{"family":"Mielke","given":"Thorsten","roleDisplay":"VerfasserIn","display":"Mielke, Thorsten","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Tarabykin, Victor","given":"Victor","family":"Tarabykin"},{"roleDisplay":"VerfasserIn","display":"Landthaler, Markus","role":"aut","family":"Landthaler","given":"Markus"},{"role":"aut","display":"Kramer, Günter","roleDisplay":"VerfasserIn","given":"Günter","family":"Kramer"},{"display":"Vida, Imre","roleDisplay":"VerfasserIn","role":"aut","family":"Vida","given":"Imre"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Selbach, Matthias","given":"Matthias","family":"Selbach"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Spahn, Christian M. T.","given":"Christian M. T.","family":"Spahn"}],"title":[{"title_sort":"Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit","title":"Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exit"}],"relHost":[{"id":{"issn":["1097-4164"],"zdb":["2001948-8"],"eki":["320416097"]},"origin":[{"publisherPlace":"[Cambridge, Mass.] ; New York, NY","dateIssuedDisp":"1997-","publisher":"Cell Press ; Elsevier","dateIssuedKey":"1997"}],"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Molecular cell","title_sort":"Molecular cell"}],"part":{"year":"2021","pages":"304-322,e1-e16","issue":"2","text":"81(2021), 2, Seite 304-322,e1-e16","volume":"81","extent":"36"},"pubHistory":["1.1997/98 -"],"recId":"320416097","language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 01.08.25"],"disp":"Protein synthesis in the developing neocortex at near-atomic resolution reveals Ebp1-mediated neuronal proteostasis at the 60S tunnel exitMolecular cell"}],"physDesc":[{"extent":"36 S."}],"name":{"displayForm":["Matthew L. Kraushar, Ferdinand Krupp, Dermot Harnett, Paul Turko, Mateusz C. Ambrozkiewicz, Thiemo Sprink, Koshi Imami, Manuel Günnigmann, Ulrike Zinnall, Carlos H. Vieira-Vieira, Theres Schaub, Agnieszka Münster-Wandowski, Jörg Bürger, Ekaterina Borisova, Hiroshi Yamamoto, Mladen-Roko Rasin, Uwe Ohler, Dieter Beule, Thorsten Mielke, Victor Tarabykin, Markus Landthaler, Günter Kramer, Imre Vida, Matthias Selbach, and Christian M.T. Spahn"]},"id":{"doi":["10.1016/j.molcel.2020.11.037"],"eki":["1770839135"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"2021"}]} | ||
| SRT | |a KRAUSHARMAPROTEINSYN2021 | ||