Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans
Induced regulatory T cells (iTreg) are a heterogeneous T-cell subset that is induced during an allo-response and down-regulates the immune response. iTreg are commonly characterized as CD4+CD25high, CD4+CD25highFoxP3+, CD4+CD25highCD127−, or CD4+CD25highFoxP3+CD127− peripheral blood lymphocytes (PBL...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
13 June 2013
|
| In: |
Transplantation proceedings
Year: 2013, Jahrgang: 45, Heft: 5, Pages: 1816-1821 |
| ISSN: | 1873-2623 |
| DOI: | 10.1016/j.transproceed.2012.10.060 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.transproceed.2012.10.060 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0041134512014133 
|
| Verfasserangaben: | H. Wang, V. Daniel, M. Sadeghi, and G. Opelz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1771821280 | ||
| 003 | DE-627 | ||
| 005 | 20220820051112.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210927s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.transproceed.2012.10.060 |2 doi | |
| 035 | |a (DE-627)1771821280 | ||
| 035 | |a (DE-599)KXP1771821280 | ||
| 035 | |a (OCoLC)1341421536 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Wang, Haihao |d 1979- |e VerfasserIn |0 (DE-588)102545457X |0 (DE-627)722302045 |0 (DE-576)370366115 |4 aut | |
| 245 | 1 | 0 | |a Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans |c H. Wang, V. Daniel, M. Sadeghi, and G. Opelz |
| 264 | 1 | |c 13 June 2013 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.09.2021 | ||
| 520 | |a Induced regulatory T cells (iTreg) are a heterogeneous T-cell subset that is induced during an allo-response and down-regulates the immune response. iTreg are commonly characterized as CD4+CD25high, CD4+CD25highFoxP3+, CD4+CD25highCD127−, or CD4+CD25highFoxP3+CD127− peripheral blood lymphocytes (PBL). In the present study, we investigated the overlap of these 4 phenotypically determined iTreg subsets in normal human individuals. PBL of 8 healthy individuals were incubated for 0 hours (Group 1) or for 16 hours in medium without (Group 2) or with phorbol 12-myristate 13-acetate (PMA)/Ionomycin (Group 3). Thereafter, proportions of PBL with Treg phenotypes were determined using 4-color flow-cytometry. All 4 Treg subsets increased strongly during polyclonal stimulation (P < .001). After stimulation, combining 2 iTreg markers, 24% of stimulated CD4+ PBL were CD25highFoxP3+, 18% CD25highCD127−, and 61% FoxP3+CD127−. Combining 3 iTreg markers, only 18% of the polyclonally stimulated CD4+ PBL were CD25highFoxP3+CD127−. Importantly, the proportion of FoxP3+CD127− PBL increased with the quantity of CD25 on stimulated CD4+ PBL and was highest in CD25high PBL (P = .002), emphasizing the relevance of CD25high as iTreg marker. Different iTreg phenotypes should not be used interchangeably because they define different iTreg subsets that overlap only in part. CD25high is the most relevant iTreg marker. These conclusions should be considered when studies and experiments involving iTreg phenotypes are compared. | ||
| 700 | 1 | |a Daniel, Volker |e VerfasserIn |0 (DE-588)1058939513 |0 (DE-627)79784418X |0 (DE-576)166309168 |4 aut | |
| 700 | 1 | |a Sadeghi, Mahmoud |d 1954- |e VerfasserIn |0 (DE-588)1013392086 |0 (DE-627)705018881 |0 (DE-576)346187966 |4 aut | |
| 700 | 1 | |a Opelz, Gerhard |d 1944- |e VerfasserIn |0 (DE-588)1026480345 |0 (DE-627)726772453 |0 (DE-576)371826527 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Transplantation proceedings |d Amsterdam [u.a.] : Elsevier Science, 1997 |g 45(2013), 5, Seite 1816-1821 |h Online-Ressource |w (DE-627)306710870 |w (DE-600)1500785-6 |w (DE-576)082436088 |x 1873-2623 |7 nnas |a Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans |
| 773 | 1 | 8 | |g volume:45 |g year:2013 |g number:5 |g pages:1816-1821 |g extent:6 |a Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.transproceed.2012.10.060 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0041134512014133 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210927 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1013392086 |a Sadeghi, Mahmoud |m 1013392086:Sadeghi, Mahmoud |d 50000 |e 50000PS1013392086 |k 0/50000/ |p 3 | ||
| 998 | |g 1058939513 |a Daniel, Volker |m 1058939513:Daniel, Volker |d 910000 |d 911600 |d 50000 |e 910000PD1058939513 |e 911600PD1058939513 |e 50000PD1058939513 |k 0/910000/ |k 1/910000/911600/ |k 0/50000/ |p 2 | ||
| 999 | |a KXP-PPN1771821280 |e 3981416406 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"given":"Haihao","family":"Wang","role":"aut","roleDisplay":"VerfasserIn","display":"Wang, Haihao"},{"family":"Daniel","given":"Volker","roleDisplay":"VerfasserIn","display":"Daniel, Volker","role":"aut"},{"display":"Sadeghi, Mahmoud","roleDisplay":"VerfasserIn","role":"aut","family":"Sadeghi","given":"Mahmoud"},{"display":"Opelz, Gerhard","roleDisplay":"VerfasserIn","role":"aut","family":"Opelz","given":"Gerhard"}],"title":[{"title":"Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans","title_sort":"Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humans"}],"recId":"1771821280","language":["eng"],"note":["Gesehen am 27.09.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["H. Wang, V. Daniel, M. Sadeghi, and G. Opelz"]},"id":{"eki":["1771821280"],"doi":["10.1016/j.transproceed.2012.10.060"]},"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"13 June 2013"}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1873-2623"],"zdb":["1500785-6"],"eki":["306710870"]},"origin":[{"dateIssuedKey":"1997","publisher":"Elsevier Science","dateIssuedDisp":"1997-","publisherPlace":"Amsterdam [u.a.]"}],"language":["eng"],"recId":"306710870","disp":"Plasticity and overlap of in vitro-induced regulatory T-cell markers in healthy humansTransplantation proceedings","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 04.08.05","Ungezählte Beil.: Supplement"],"part":{"text":"45(2013), 5, Seite 1816-1821","volume":"45","extent":"6","year":"2013","issue":"5","pages":"1816-1821"},"pubHistory":["Nachgewiesen 29.1997 -"],"title":[{"title":"Transplantation proceedings","title_sort":"Transplantation proceedings"}]}],"physDesc":[{"extent":"6 S."}]} | ||
| SRT | |a WANGHAIHAOPLASTICITY1320 | ||