Streptamer versus tetramer-based selection of functional cytomegalovirus-specific T cells
Background/Purpose - Cytomegalovirus (CMV) disease constitutes a serious complication after stem cell transplantation and has been treated by adoptive transfer of donor-derived CMV-specific CD8+ T cells. CMV-specific CD8+ T cells were selected by multimers, and the technologies may alter the functio...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
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| In: |
Journal of the Formosan Medical Association
Year: 2013, Jahrgang: 112, Heft: 6, Pages: 338-345 |
| ISSN: | 1876-0821 |
| DOI: | 10.1016/j.jfma.2012.02.020 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jfma.2012.02.020 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0929664612001866 
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| Verfasserangaben: | Xin-Chao Wang, Hua Pang, Xun Xu, Anita Schmitt, Mathias Freund, Michael Schmitt, Bao-An Chen |
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| 520 | |a Background/Purpose - Cytomegalovirus (CMV) disease constitutes a serious complication after stem cell transplantation and has been treated by adoptive transfer of donor-derived CMV-specific CD8+ T cells. CMV-specific CD8+ T cells were selected by multimers, and the technologies may alter the function of these T cells. Therefore, here we evaluated the impact of multimer reagents on the function of CD8+ T lymphocytes. - Methods - CMV-specific CD8+ T cells were purified from the peripheral blood of donors using tetra- and streptamer technologies. The functional status of purified CMV-specific CD8+ T cells was assessed by multiparametric immunophenotyping and carboxyfluorescein succinimidyl ester proliferation assays as well as by enzyme-linked immunospot assays. - Results - A similar percentage of CMV-specific CD8+ T cells could be purified by both tetra- (90%) and streptamer (92%) technologies. That constitutes a 30- to 50-fold concentration of CMV-specific CD8+CD45RA+CCR7−effector T cells. Selected cells secreted interferon-gamma and granzyme B upon stimulation with CMVpp65 peptide, thus demonstrating their functionality. - Conclusion - Our study demonstrated that both tetra- and streptamer technologies can be used to purify CMV-specific cytotoxic CD8+ T cells for adoptive T-cell transfer. Both multimer technologies did not have any negative influence on the proliferation of selected T cells. Importantly, streptamer technology is available at good manufacturing practice level. | ||
| 650 | 4 | |a adoptive immunotherapy | |
| 650 | 4 | |a cytomegalovirus | |
| 650 | 4 | |a cytomegalovirus-specific T cells | |
| 650 | 4 | |a stem cell transplantation | |
| 650 | 4 | |a streptamers | |
| 650 | 4 | |a tetramer | |
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| 700 | 1 | |a Xu, Xun |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Chen, Bao-An |e VerfasserIn |4 aut | |
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