Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness
Angiogenesis is an essential process during development. Abnormal angiogenesis also contributes to many disease conditions such as tumor and retinal diseases. Previous studies have established the Hippo signaling pathway effector Yes-associated protein (YAP) as a crucial regulator of angiogenesis. I...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
13 May 2021
|
| In: |
Frontiers in cell and developmental biology
Year: 2021, Volume: 9, Pages: 1-14 |
| ISSN: | 2296-634X |
| DOI: | 10.3389/fcell.2021.675562 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3389/fcell.2021.675562 Verlag, lizenzpflichtig, Volltext: https://www.frontiersin.org/article/10.3389/fcell.2021.675562 
|
| Author Notes: | Xiao Jiang, Jiandong Hu, Ziru Wu, Sarah Trusso Cafarello, Mario Di Matteo, Ying Shen, Xue Dong, Heike Adler, Massimiliano Mazzone, Carmen Ruiz de Almodovar and Xiaohong Wang |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1772583642 | ||
| 003 | DE-627 | ||
| 005 | 20230427055924.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211006s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fcell.2021.675562 |2 doi | |
| 035 | |a (DE-627)1772583642 | ||
| 035 | |a (DE-599)KXP1772583642 | ||
| 035 | |a (OCoLC)1341421662 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Jiang, Xiao |e VerfasserIn |0 (DE-588)1242570861 |0 (DE-627)177255295X |4 aut | |
| 245 | 1 | 0 | |a Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness |c Xiao Jiang, Jiandong Hu, Ziru Wu, Sarah Trusso Cafarello, Mario Di Matteo, Ying Shen, Xue Dong, Heike Adler, Massimiliano Mazzone, Carmen Ruiz de Almodovar and Xiaohong Wang |
| 264 | 1 | |c 13 May 2021 | |
| 300 | |a 14 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.10.2021 | ||
| 520 | |a Angiogenesis is an essential process during development. Abnormal angiogenesis also contributes to many disease conditions such as tumor and retinal diseases. Previous studies have established the Hippo signaling pathway effector Yes-associated protein (YAP) as a crucial regulator of angiogenesis. In ECs, activated YAP promotes endothelial cell proliferation, migration and sprouting. YAP activity is regulated by vascular endothelial growth factor (VEGF) and mechanical cues such as extracellular matrix (ECM) stiffness. However, it is unclear how VEGF or ECM stiffness signal to YAP, especially how dephosphorylation of YAP occurs in response to VEGF stimulus or ECM stiffening. Here, we show that protein phosphatase 2A (PP2A) is required for this process. Blocking PP2A activity abolishes VEGF or ECM stiffening mediated YAP activation. Systemic administration of a PP2A inhibitor suppresses YAP activity in blood vessels in developmental and pathological angiogenesis mouse models. Consistently, PP2A inhibitor also inhibits sprouting angiogenesis. Mechanistically, PP2A directly interacts with YAP, and this interaction requires proper cytoskeleton dynamics. These findings identify PP2A as a crucial mediator of YAP activation in ECs and hence as an important regulator of angiogenesis. | ||
| 700 | 1 | |a Hu, Jiandong |e VerfasserIn |4 aut | |
| 700 | 1 | |a Wu, Ziru |e VerfasserIn |4 aut | |
| 700 | 1 | |a Cafarello, Sarah Trusso |e VerfasserIn |4 aut | |
| 700 | 1 | |a Di Matteo, Mario |e VerfasserIn |4 aut | |
| 700 | 1 | |a Shen, Ying |d 1987- |e VerfasserIn |0 (DE-588)1144909899 |0 (DE-627)1005039518 |0 (DE-576)495511676 |4 aut | |
| 700 | 1 | |a Dong, Xue |e VerfasserIn |4 aut | |
| 700 | 1 | |a Adler, Heike |d 1971- |e VerfasserIn |0 (DE-588)1161624236 |0 (DE-627)1024912248 |0 (DE-576)506771482 |4 aut | |
| 700 | 1 | |a Mazzone, Massimiliano |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ruiz de Almodóvar, Carmen |e VerfasserIn |0 (DE-588)1073942848 |0 (DE-627)830007776 |0 (DE-576)435417479 |4 aut | |
| 700 | 1 | |a Wang, Xiaohong |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in cell and developmental biology |d Lausanne : Frontiers Media, 2013 |g 9(2021) vom: Mai, Artikel-ID 675562, Seite 1-14 |h Online-Ressource |w (DE-627)770398138 |w (DE-600)2737824-X |w (DE-576)394650182 |x 2296-634X |7 nnas |a Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness |
| 773 | 1 | 8 | |g volume:9 |g year:2021 |g month:05 |g elocationid:675562 |g pages:1-14 |g extent:14 |a Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fcell.2021.675562 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/article/10.3389/fcell.2021.675562 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211006 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1073942848 |a Ruiz de Almodóvar, Carmen |m 1073942848:Ruiz de Almodóvar, Carmen |d 60000 |d 65000 |e 60000PR1073942848 |e 65000PR1073942848 |k 0/60000/ |k 1/60000/65000/ |p 10 | ||
| 998 | |g 1161624236 |a Adler, Heike |m 1161624236:Adler, Heike |d 60000 |d 65000 |e 60000PA1161624236 |e 65000PA1161624236 |k 0/60000/ |k 1/60000/65000/ |p 8 | ||
| 998 | |g 1144909899 |a Shen, Ying |m 1144909899:Shen, Ying |d 60000 |d 65000 |e 60000PS1144909899 |e 65000PS1144909899 |k 0/60000/ |k 1/60000/65000/ |p 6 | ||
| 999 | |a KXP-PPN1772583642 |e 3984910258 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness","title_sort":"Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffness"}],"person":[{"display":"Jiang, Xiao","roleDisplay":"VerfasserIn","role":"aut","family":"Jiang","given":"Xiao"},{"family":"Hu","given":"Jiandong","roleDisplay":"VerfasserIn","display":"Hu, Jiandong","role":"aut"},{"role":"aut","display":"Wu, Ziru","roleDisplay":"VerfasserIn","given":"Ziru","family":"Wu"},{"role":"aut","display":"Cafarello, Sarah Trusso","roleDisplay":"VerfasserIn","given":"Sarah Trusso","family":"Cafarello"},{"given":"Mario","family":"Di Matteo","role":"aut","display":"Di Matteo, Mario","roleDisplay":"VerfasserIn"},{"given":"Ying","family":"Shen","role":"aut","display":"Shen, Ying","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","display":"Dong, Xue","role":"aut","family":"Dong","given":"Xue"},{"roleDisplay":"VerfasserIn","display":"Adler, Heike","role":"aut","family":"Adler","given":"Heike"},{"role":"aut","display":"Mazzone, Massimiliano","roleDisplay":"VerfasserIn","given":"Massimiliano","family":"Mazzone"},{"role":"aut","display":"Ruiz de Almodóvar, Carmen","roleDisplay":"VerfasserIn","given":"Carmen","family":"Ruiz de Almodóvar"},{"display":"Wang, Xiaohong","roleDisplay":"VerfasserIn","role":"aut","family":"Wang","given":"Xiaohong"}],"language":["eng"],"recId":"1772583642","note":["Gesehen am 06.10.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"eki":["1772583642"],"doi":["10.3389/fcell.2021.675562"]},"origin":[{"dateIssuedKey":"2021","dateIssuedDisp":"13 May 2021"}],"name":{"displayForm":["Xiao Jiang, Jiandong Hu, Ziru Wu, Sarah Trusso Cafarello, Mario Di Matteo, Ying Shen, Xue Dong, Heike Adler, Massimiliano Mazzone, Carmen Ruiz de Almodovar and Xiaohong Wang"]},"relHost":[{"origin":[{"publisherPlace":"Lausanne","publisher":"Frontiers Media","dateIssuedKey":"2013","dateIssuedDisp":"2013-"}],"id":{"issn":["2296-634X"],"zdb":["2737824-X"],"eki":["770398138"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Frontiers in cell and developmental biology","title":"Frontiers in cell and developmental biology"}],"pubHistory":["2013 -"],"titleAlt":[{"title":"FCELL"}],"part":{"pages":"1-14","year":"2021","extent":"14","text":"9(2021) vom: Mai, Artikel-ID 675562, Seite 1-14","volume":"9"},"disp":"Protein phosphatase 2A mediates YAP activation in endothelial cells upon VEGF stimulation and matrix stiffnessFrontiers in cell and developmental biology","note":["Gesehen am 04.06.20"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"770398138","language":["eng"]}],"physDesc":[{"extent":"14 S."}]} | ||
| SRT | |a JIANGXIAOHPROTEINPHO1320 | ||