Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins
The Co3+-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni2+ or...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
05 June 2013
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| In: |
Angewandte Chemie. International edition
Year: 2013, Volume: 52, Issue: 29, Pages: 7593-7596 |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201210317 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/anie.201210317 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201210317 
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| Author Notes: | Seraphine V. Wegner and Joachim P. Spatz |
| Summary: | The Co3+-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni2+ or Co2+ complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces. |
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| Item Description: | Gesehen am 18.10.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201210317 |