Cover Image

Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells

In situ cryo-electron tomography of cryo-focused ion beam (cryo-FIB) milled cells enables the study of cellular organelles in unperturbed conditions and close to the molecular resolution. However, due to the crowdedness of the cellular environment, the identification of individual macromolecular com...

Full description

Saved in:
Bibliographic Details
Main Authors: Klein, Steffen (Author) , Wachsmuth-Melm, Moritz (Author) , Winter, Sophie L. (Author) , Kolovou, Androniki (Author) , Chlanda, Petr (Author)
Format: Chapter/Article
Language:English
Published: 2021
In: Correlative light and electron microscopy ; 4
Year: 2021, Pages: 273-302
Online Access:Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0091679X20302156
Get full text
Author Notes:Steffen Klein, Moritz Wachsmuth-Melm, Sophie L. Winter, Androniki Kolovou, and Petr Chlanda

MARC

LEADER 00000caa a2200000 c 4500
001 1777171903
003 DE-627
005 20220820072937.0
007 cr uuu---uuuuu
008 211110s2021 xx |||||o 00| ||eng c
035 |a (DE-627)1777171903 
035 |a (DE-599)KXP1777171903 
035 |a (OCoLC)1341423927 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Klein, Steffen  |d 1989-  |e VerfasserIn  |0 (DE-588)1209782782  |0 (DE-627)1697757294  |4 aut 
245 1 0 |a Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells  |c Steffen Klein, Moritz Wachsmuth-Melm, Sophie L. Winter, Androniki Kolovou, and Petr Chlanda 
264 1 |c 2021 
300 |a 30 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 10.11.2021 
520 |a In situ cryo-electron tomography of cryo-focused ion beam (cryo-FIB) milled cells enables the study of cellular organelles in unperturbed conditions and close to the molecular resolution. However, due to the crowdedness of the cellular environment, the identification of individual macromolecular complexes either on organelles or inside the cytosol in cryo-electron tomograms is challenging. Cryo-correlative light and electron microscopy (cryo-CLEM) employs a fluorescently labeled feature of interest imaged by cryo-light microscopy that is correlated to cryo-electron microscopy maps of cryo-FIB milled lamellae using correlation markers discernable by both imaging methods. Here, we provide a protocol for a post-correlation on-lamella cryo-CLEM approach for localization of fluorescently labeled organelles of interest in cryo-lamellae after cryo-FIB milling and tomography of adherent plunge frozen cells. 
650 4 |a CLEM 
650 4 |a Correlation 
650 4 |a Cryo-ET 
650 4 |a FIB-milling 
650 4 |a FIB/SEM 
650 4 |a In situ 
700 1 |a Wachsmuth-Melm, Moritz  |d 1995-  |e VerfasserIn  |0 (DE-588)1224904478  |0 (DE-627)1744397406  |4 aut 
700 1 |a Winter, Sophie L.  |d 1992-  |e VerfasserIn  |0 (DE-588)1222681641  |0 (DE-627)1741844622  |4 aut 
700 1 |a Kolovou, Androniki  |e VerfasserIn  |0 (DE-588)1114896241  |0 (DE-627)869380974  |0 (DE-576)477559999  |4 aut 
700 1 |a Chlanda, Petr  |e VerfasserIn  |0 (DE-588)141254823  |0 (DE-627)626165261  |0 (DE-576)323011152  |4 aut 
773 0 8 |i Enthalten in  |t Correlative light and electron microscopy ; 4  |b First edition  |d Cambridge, MA : Academic Press, an imprint of Elsevier, 2021  |g (2021), Seite 273-302  |h 1 Online-Ressource (xix, 430 Seiten)  |w (DE-627)1756650365  |z 9780128220580  |7 nnam  |a Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells 
773 1 8 |g year:2021  |g pages:273-302  |g extent:30  |a Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells 
856 4 0 |u https://www.sciencedirect.com/science/article/pii/S0091679X20302156  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20211110 
993 |a BookComponentPart 
994 |a 2021 
998 |g 141254823  |a Chlanda, Petr  |m 141254823:Chlanda, Petr  |d 910000  |d 911700  |e 910000PC141254823  |e 911700PC141254823  |k 0/910000/  |k 1/910000/911700/  |p 5  |y j 
998 |g 1114896241  |a Kolovou, Androniki  |m 1114896241:Kolovou, Androniki  |d 700000  |d 716000  |e 700000PK1114896241  |e 716000PK1114896241  |k 0/700000/  |k 1/700000/716000/  |p 4 
998 |g 1222681641  |a Winter, Sophie L.  |m 1222681641:Winter, Sophie L.  |d 910000  |d 911700  |e 910000PW1222681641  |e 911700PW1222681641  |k 0/910000/  |k 1/910000/911700/  |p 3 
998 |g 1224904478  |a Wachsmuth-Melm, Moritz  |m 1224904478:Wachsmuth-Melm, Moritz  |d 910000  |d 911700  |e 910000PW1224904478  |e 911700PW1224904478  |k 0/910000/  |k 1/910000/911700/  |p 2 
998 |g 1209782782  |a Klein, Steffen  |m 1209782782:Klein, Steffen  |d 910000  |d 911700  |e 910000PK1209782782  |e 911700PK1209782782  |k 0/910000/  |k 1/910000/911700/  |p 1  |x j 
999 |a KXP-PPN1777171903  |e 400135750X 
BIB |a Y 
JSO |a {"id":{"eki":["1777171903"]},"type":{"bibl":"chapter","media":"Online-Ressource"},"title":[{"title":"Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells","title_sort":"Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells"}],"relHost":[{"physDesc":[{"noteIll":"Illustrationen","extent":"1 Online-Ressource (xix, 430 Seiten)"}],"title":[{}],"relMultPart":[{"origin":[{"dateIssuedDisp":"2021-","publisher":"Academic Press, an imprint of Elsevier","dateIssuedKey":"2021","publisherPlace":"Cambridge, MA ; San Diego, CA$aOxford$aLondon"}],"recId":"1756649456","part":{"number_sort":["4"],"number":["4"]},"language":["eng"],"title":[{"title_sort":"Correlative light and electron microscopy","title":"Correlative light and electron microscopy"}],"type":{"media":"Online-Ressource","bibl":"edited-book"},"id":{"eki":["1756649456"]},"person":[{"given":"Thomas","role":"edt","display":"Müller-Reichert, Thomas","family":"Müller-Reichert"},{"given":"Paul","role":"ctb","display":"Verkade, Paul","family":"Verkade"}],"name":{"displayForm":["edited by Thomas Müller-Reichert, Paul Verkade"]},"ga":1},{"part":{"number":["volume 162"],"number_sort":["162"]},"disp":"Methods in cell biology","ga":1,"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Methods in cell biology","title":"Methods in cell biology"}],"type":{"media":"Online-Ressource","bibl":"serial"},"id":{"zdb":["2257731-2"],"issn":["0091-679X"],"eki":["520196031"]},"origin":[{"publisher":"Elsevier","dateIssuedDisp":"2004-","dateIssuedKey":"2004","publisherPlace":"New York, NY [u.a.]"}],"dispAlt":"Methods in cell biology","language":["eng"],"recId":"520196031","pubHistory":["Nachgewiesen 76.2004 -"]}],"type":{"bibl":"book","media":"Online-Ressource"},"id":{"isbn":["9780128220580"],"eki":["1756650365"]},"origin":[{"dateIssuedDisp":"2021","publisher":"Academic Press, an imprint of Elsevier","dateIssuedKey":"2021","editionNo":1,"publisherPlace":"Cambridge, MA ; San Diego, CA$aOxford$aLondon","edition":"First edition"}],"language":["eng"],"part":{"year":"2021","text":"(2021), Seite 273-302","extent":"30","pages":"273-302"},"recId":"1756650365","disp":"Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cellsCorrelative light and electron microscopy ; 4"}],"physDesc":[{"extent":"30 S."}],"person":[{"role":"aut","given":"Steffen","family":"Klein","display":"Klein, Steffen"},{"given":"Moritz","role":"aut","display":"Wachsmuth-Melm, Moritz","family":"Wachsmuth-Melm"},{"role":"aut","given":"Sophie L.","family":"Winter","display":"Winter, Sophie L."},{"role":"aut","given":"Androniki","family":"Kolovou","display":"Kolovou, Androniki"},{"family":"Chlanda","display":"Chlanda, Petr","role":"aut","given":"Petr"}],"name":{"displayForm":["Steffen Klein, Moritz Wachsmuth-Melm, Sophie L. Winter, Androniki Kolovou, and Petr Chlanda"]},"recId":"1777171903","language":["eng"],"note":["Gesehen am 10.11.2021"],"origin":[{"dateIssuedDisp":"2021","dateIssuedKey":"2021"}]} 
SRT |a KLEINSTEFFCRYOCORREL2021 

Similar Items