LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils
A growing body of evidence suggests that innate immune cells can respond in a memory-like (adaptive) fashion, which is referred to as trained immunity. Only few in vivo studies have shown training effects in neutrophils; however, no in vitro setup has been established to study the induction of train...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
10 September 2021
|
| In: |
International journal of molecular sciences
Year: 2021, Jahrgang: 22, Heft: 18, Pages: 1-17 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22189803 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ijms22189803 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1422-0067/22/18/9803 
|
| Verfasserangaben: | Trim Lajqi, Maylis Braun, Simon Alexander Kranig, David Frommhold, Johannes Pöschl and Hannes Hudalla |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1777292425 | ||
| 003 | DE-627 | ||
| 005 | 20220820073612.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211111s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/ijms22189803 |2 doi | |
| 035 | |a (DE-627)1777292425 | ||
| 035 | |a (DE-599)KXP1777292425 | ||
| 035 | |a (OCoLC)1341423827 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Lajqi, Trim |d 1990- |e VerfasserIn |0 (DE-588)1184939764 |0 (DE-627)1663824916 |4 aut | |
| 245 | 1 | 0 | |a LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils |c Trim Lajqi, Maylis Braun, Simon Alexander Kranig, David Frommhold, Johannes Pöschl and Hannes Hudalla |
| 264 | 1 | |c 10 September 2021 | |
| 300 | |a 17 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 11.11.2021 | ||
| 520 | |a A growing body of evidence suggests that innate immune cells can respond in a memory-like (adaptive) fashion, which is referred to as trained immunity. Only few in vivo studies have shown training effects in neutrophils; however, no in vitro setup has been established to study the induction of trained immunity or tolerance in neutrophils by microbial agents. In light of their short lifespan (up to 48 h), we suggest to use the term trained sensitivity for neutrophils in an in vitro setting. Here, we firstly describe a feasible two-hit model, using different doses of lipopolysaccharide (LPS) in bone marrow neutrophils. We found that low doses (10 pg/mL) induce pro-inflammatory activation (trained sensitivity), whereas priming with high doses (100 ng/mL) leads to suppression of pro-inflammatory mediators such as TNF-α or IL-6 (tolerance) (p < 0.05). On a functional level, trained neutrophils displayed increased phagocytic activity and LFA-1 expression as well as migrational capacity and CD11a expression, whereas tolerant neutrophils show contrasting effects in vitro. Mechanistically, TLR4/MyD88/PI3Ks regulate the activation of p65, which controls memory-like responses in mouse bone marrow neutrophils (p < 0.05). Our results open a new window for further in vitro studies on memory-like inflammatory responses of short-lived innate immune cells such as neutrophils. | ||
| 650 | 4 | |a LPS | |
| 650 | 4 | |a migration | |
| 650 | 4 | |a neutrophils | |
| 650 | 4 | |a phagocytosis | |
| 650 | 4 | |a priming | |
| 650 | 4 | |a tolerance | |
| 650 | 4 | |a trained sensitivity | |
| 700 | 1 | |a Jungwirth, Maylis |d 1990- |e VerfasserIn |0 (DE-588)1202824609 |0 (DE-627)168733935X |4 aut | |
| 700 | 1 | |a Kranig, Simon A. |d 1985- |e VerfasserIn |0 (DE-588)1059560062 |0 (DE-627)798630272 |0 (DE-576)415693691 |4 aut | |
| 700 | 1 | |a Frommhold, David |d 1974- |e VerfasserIn |0 (DE-588)128786787 |0 (DE-627)379685779 |0 (DE-576)297332643 |4 aut | |
| 700 | 1 | |a Pöschl, Johannes |e VerfasserIn |0 (DE-588)1021936847 |0 (DE-627)715839977 |0 (DE-576)36384757X |4 aut | |
| 700 | 1 | |a Hudalla, Hannes |d 1985- |e VerfasserIn |0 (DE-588)1053957645 |0 (DE-627)790859149 |0 (DE-576)40992072X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of molecular sciences |d Basel : Molecular Diversity Preservation International, 2000 |g 22(2021), 18, Artikel-ID 9803, Seite 1-17 |h Online-Ressource |w (DE-627)316340715 |w (DE-600)2019364-6 |w (DE-576)281194653 |x 1422-0067 |7 nnas |a LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils |
| 773 | 1 | 8 | |g volume:22 |g year:2021 |g number:18 |g elocationid:9803 |g pages:1-17 |g extent:17 |a LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils |
| 856 | 4 | 0 | |u https://doi.org/10.3390/ijms22189803 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/1422-0067/22/18/9803 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211111 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1053957645 |a Hudalla, Hannes |m 1053957645:Hudalla, Hannes |d 910000 |d 910500 |e 910000PH1053957645 |e 910500PH1053957645 |k 0/910000/ |k 1/910000/910500/ |p 6 |y j | ||
| 998 | |g 1021936847 |a Pöschl, Johannes |m 1021936847:Pöschl, Johannes |d 910000 |d 910500 |d 50000 |e 910000PP1021936847 |e 910500PP1021936847 |e 50000PP1021936847 |k 0/910000/ |k 1/910000/910500/ |k 0/50000/ |p 5 | ||
| 998 | |g 128786787 |a Frommhold, David |m 128786787:Frommhold, David |d 50000 |e 50000PF128786787 |k 0/50000/ |p 4 | ||
| 998 | |g 1059560062 |a Kranig, Simon A. |m 1059560062:Kranig, Simon A. |d 910000 |d 910500 |e 910000PK1059560062 |e 910500PK1059560062 |k 0/910000/ |k 1/910000/910500/ |p 3 | ||
| 998 | |g 1202824609 |a Jungwirth, Maylis |m 1202824609:Jungwirth, Maylis |d 910000 |d 910500 |e 910000PJ1202824609 |e 910500PJ1202824609 |k 0/910000/ |k 1/910000/910500/ |p 2 | ||
| 998 | |g 1184939764 |a Lajqi, Trim |m 1184939764:Lajqi, Trim |d 910000 |d 910500 |e 910000PL1184939764 |e 910500PL1184939764 |k 0/910000/ |k 1/910000/910500/ |p 1 |x j | ||
| 999 | |a KXP-PPN1777292425 |e 4001650215 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"display":"Lajqi, Trim","family":"Lajqi","given":"Trim","role":"aut"},{"role":"aut","given":"Maylis","family":"Jungwirth","display":"Jungwirth, Maylis"},{"display":"Kranig, Simon A.","family":"Kranig","given":"Simon A.","role":"aut"},{"family":"Frommhold","display":"Frommhold, David","given":"David","role":"aut"},{"given":"Johannes","role":"aut","display":"Pöschl, Johannes","family":"Pöschl"},{"role":"aut","given":"Hannes","family":"Hudalla","display":"Hudalla, Hannes"}],"language":["eng"],"note":["Gesehen am 11.11.2021"],"origin":[{"dateIssuedDisp":"10 September 2021","dateIssuedKey":"2021"}],"title":[{"title_sort":"LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils","title":"LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophils"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"recId":"1777292425","physDesc":[{"extent":"17 S."}],"name":{"displayForm":["Trim Lajqi, Maylis Braun, Simon Alexander Kranig, David Frommhold, Johannes Pöschl and Hannes Hudalla"]},"id":{"doi":["10.3390/ijms22189803"],"eki":["1777292425"]},"relHost":[{"id":{"zdb":["2019364-6"],"eki":["316340715"],"issn":["1422-0067","1661-6596"]},"pubHistory":["1.2000 -"],"recId":"316340715","physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 17.09.20"],"origin":[{"publisher":"Molecular Diversity Preservation International","dateIssuedKey":"2000","dateIssuedDisp":"2000-","publisherPlace":"Basel"}],"titleAlt":[{"title":"IJMS"}],"title":[{"title":"International journal of molecular sciences","title_sort":"International journal of molecular sciences"}],"part":{"issue":"18","pages":"1-17","text":"22(2021), 18, Artikel-ID 9803, Seite 1-17","year":"2021","volume":"22","extent":"17"},"disp":"LPS induces opposing memory-like inflammatory responses in mouse bone marrow neutrophilsInternational journal of molecular sciences"}]} | ||
| SRT | |a LAJQITRIMJLPSINDUCES1020 | ||