Epsin but not AP-2 supports reconstitution of endocytic clathrin-coated vesicles
Formation of clathrin-coated vesicles (CCVs) in receptor-mediated endocytosis is a mechanistically well-established process, in which clathrin, the adaptor protein complex AP-2, and the large GTPase dynamin play crucial roles. In order to obtain more mechanistic insight into this process, here we es...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2020
|
| In: |
FEBS letters
Year: 2020, Jahrgang: 594, Heft: 14, Pages: 2227-2239 |
| ISSN: | 1873-3468 |
| DOI: | 10.1002/1873-3468.13801 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/1873-3468.13801 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13801 
|
| Verfasserangaben: | Jan Brod, Andrea Hellwig and Felix T. Wieland |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1777369487 | ||
| 003 | DE-627 | ||
| 005 | 20220208225616.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211112s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/1873-3468.13801 |2 doi | |
| 035 | |a (DE-627)1777369487 | ||
| 035 | |a (DE-599)KXP1777369487 | ||
| 035 | |a (OCoLC)1295680232 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Brod, Jan |e VerfasserIn |0 (DE-588)1168297001 |0 (DE-627)1031943277 |0 (DE-576)511509804 |4 aut | |
| 245 | 1 | 0 | |a Epsin but not AP-2 supports reconstitution of endocytic clathrin-coated vesicles |c Jan Brod, Andrea Hellwig and Felix T. Wieland |
| 264 | 1 | |c 2020 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a First published: 26 April 2020 | ||
| 500 | |a Gesehen am 12.11.2021 | ||
| 520 | |a Formation of clathrin-coated vesicles (CCVs) in receptor-mediated endocytosis is a mechanistically well-established process, in which clathrin, the adaptor protein complex AP-2, and the large GTPase dynamin play crucial roles. In order to obtain more mechanistic insight into this process, here we established a giant unilamellar vesicle (GUV)-based in vitro CCV reconstitution system with chemically defined components and the full-length recombinant proteins clathrin, AP-2, epsin-1, and dynamin-2. Our results support the predominant model in which hydrolysis of GTP by dynamin is a prerequisite to generate CCVs. Strikingly, in this system at near physiological concentrations of reagents, epsin-1 alone does not have the propensity for scission but is required for bud formation, whereas AP-2 and clathrin are not sufficient. Thus, our study reveals that epsin-1 is an important factor for the maturation of clathrin coated buds, a prerequisite for vesicle generation. | ||
| 650 | 4 | |a clathrin-coated vesicle | |
| 650 | 4 | |a endocytosis | |
| 650 | 4 | |a membrane scission | |
| 650 | 4 | |a vesicle reconstitution | |
| 650 | 4 | |a vesicular transport | |
| 700 | 1 | |a Hellwig, Andrea |e VerfasserIn |0 (DE-588)1055984356 |0 (DE-627)79302112X |0 (DE-576)412003775 |4 aut | |
| 700 | 1 | |a Wieland, Felix T. |d 1948- |e VerfasserIn |0 (DE-588)1020541881 |0 (DE-627)69125365X |0 (DE-576)360684009 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |a Vereinigung der Europäischen Biochemischen Gesellschaften |t FEBS letters |d Chichester : Wiley, 1968 |g 594(2020), 14, Seite 2227-2239 |h Online-Ressource |w (DE-627)253779669 |w (DE-600)1460391-3 |w (DE-576)07279383X |x 1873-3468 |7 nnas |
| 773 | 1 | 8 | |g volume:594 |g year:2020 |g number:14 |g pages:2227-2239 |g extent:13 |a Epsin but not AP-2 supports reconstitution of endocytic clathrin-coated vesicles |
| 856 | 4 | 0 | |u https://doi.org/10.1002/1873-3468.13801 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13801 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211112 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1020541881 |a Wieland, Felix T. |m 1020541881:Wieland, Felix T. |d 50000 |e 50000PW1020541881 |k 0/50000/ |p 3 |y j | ||
| 998 | |g 1055984356 |a Hellwig, Andrea |m 1055984356:Hellwig, Andrea |d 700000 |d 712000 |d 712100 |e 700000PH1055984356 |e 712000PH1055984356 |e 712100PH1055984356 |k 0/700000/ |k 1/700000/712000/ |k 2/700000/712000/712100/ |p 2 | ||
| 998 | |g 1168297001 |a Brod, Jan |m 1168297001:Brod, Jan |p 1 |x j | ||
| 999 | |a KXP-PPN1777369487 |e 4001841460 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"2020"}],"note":["First published: 26 April 2020","Gesehen am 12.11.2021"],"recId":"1777369487","relHost":[{"language":["eng"],"name":{"displayForm":["Federation of European Biochemical Societies. Ed. M. Avron [u.a.]"]},"id":{"issn":["1873-3468"],"doi":["10.1002/(ISSN)1873-3468"],"zdb":["1460391-3"],"eki":["253779669"]},"pubHistory":["1.1968 -"],"origin":[{"publisher":"Wiley ; North-Holland Publ. Co. ; Elsevier","dateIssuedDisp":"1968-","dateIssuedKey":"1968","publisherPlace":"Chichester ; Amsterdam ; Amsterdam [u.a.]"}],"note":["Gesehen am 18.12.2019","Fortsetzung der Druck-Ausgabe"],"recId":"253779669","type":{"bibl":"periodical","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"disp":"Vereinigung der Europäischen Biochemischen GesellschaftenFEBS letters","part":{"extent":"13","volume":"594","issue":"14","pages":"2227-2239","year":"2020","text":"594(2020), 14, Seite 2227-2239"},"title":[{"title_sort":"FEBS letters","subtitle":"for the rapid publication of short reports in biochemistry, biophysics, and molecular biology","title":"FEBS letters"}],"corporate":[{"role":"aut","display":"Vereinigung der Europäischen Biochemischen Gesellschaften"}]}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"language":["eng"],"name":{"displayForm":["Jan Brod, Andrea Hellwig and Felix T. Wieland"]},"person":[{"role":"aut","display":"Brod, Jan","given":"Jan","family":"Brod"},{"family":"Hellwig","role":"aut","display":"Hellwig, Andrea","given":"Andrea"},{"family":"Wieland","role":"aut","given":"Felix T.","display":"Wieland, Felix T."}],"title":[{"title_sort":"Epsin but not AP-2 supports reconstitution of endocytic clathrin-coated vesicles","title":"Epsin but not AP-2 supports reconstitution of endocytic clathrin-coated vesicles"}],"id":{"eki":["1777369487"],"doi":["10.1002/1873-3468.13801"]},"physDesc":[{"extent":"13 S."}]} | ||
| SRT | |a BRODJANHELEPSINBUTNO2020 | ||