Direct-PCR from rectal swabs and environmental reservoirs: a fast and efficient alternative to detect blaOXA-48 carbapenemase genes in an Enterobacter cloacae outbreak setting
Carbapenemase-producing bacteria are a risk factor in clinical settings worldwide. The aim of the study was to accelerate the time to results during an outbreak situation with blaOXA-48-positive Enterobacter cloacae by using a real-time multiplex quantitative PCR (qPCR) directly on rectal swab speci...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2022
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| In: |
Environmental research
Year: 2022, Jahrgang: 203, Pages: 1-5 |
| ISSN: | 1096-0953 |
| DOI: | 10.1016/j.envres.2021.111808 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.envres.2021.111808 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0013935121011026 
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| Verfasserangaben: | Katja Probst, Sébastien Boutin, Isabel Späth, Martin Scherrer, Nicole Henny, Delal Sahin, Alexandra Heininger, Klaus Heeg, Dennis Nurjadi |
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| 520 | |a Carbapenemase-producing bacteria are a risk factor in clinical settings worldwide. The aim of the study was to accelerate the time to results during an outbreak situation with blaOXA-48-positive Enterobacter cloacae by using a real-time multiplex quantitative PCR (qPCR) directly on rectal swab specimens and on wastewater samples to detect carbapenemase-producing bacteria. Thus, we analyzed 681 rectal swabs and 947 environmental samples during a five-month period by qPCR and compared the results to culture screening. The qPCR showed a sensitivity of 100% by testing directly from rectal swabs and was in ten cases more sensitive than the culture-based methods. Environmental screening for blaOXA-48-carbapenemase genes by qPCR revealed reservoirs of different carbapenemase genes that are potential sources of transmission and might lead to new outbreaks. The rapid identification of patients colonized with those isolates and screening of the hospital environment is essential for earlier patient treatment and eliminating potential sources of nosocomial infections. | ||
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| 650 | 4 | |a Carbapenemase | |
| 650 | 4 | |a Environmental screening | |
| 650 | 4 | |a Real-time multiplex PCR | |
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