Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing
The SNAP-ADAR tool enables precise and efficient A-to-I RNA editing in a guideRNA-dependent manner by applying the self-labeling SNAP-tag enzyme to generate RNA-guided editases in cell culture. Here, we extend this platform by combining the SNAP-tagged tool with further effectors steered by the orth...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 July 2021
|
| In: |
Nucleic acids research
Year: 2021, Jahrgang: 49, Heft: 16, Pages: 1-12 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkab541 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gkab541
|
| Verfasserangaben: | Anna S. Stroppel, Ngadhnjim Latifi, Alfred Hanswillemenke, Rafail Nikolaos Tasakis, F. Nina Papavasiliou and Thorsten Stafforst |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1778218199 | ||
| 003 | DE-627 | ||
| 005 | 20220820081109.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211122s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nar/gkab541 |2 doi | |
| 035 | |a (DE-627)1778218199 | ||
| 035 | |a (DE-599)KXP1778218199 | ||
| 035 | |a (OCoLC)1341430885 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Stroppel, Anna |e VerfasserIn |0 (DE-588)1246210452 |0 (DE-627)1778218970 |4 aut | |
| 245 | 1 | 0 | |a Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing |c Anna S. Stroppel, Ngadhnjim Latifi, Alfred Hanswillemenke, Rafail Nikolaos Tasakis, F. Nina Papavasiliou and Thorsten Stafforst |
| 264 | 1 | |c 01 July 2021 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.11.2021 | ||
| 520 | |a The SNAP-ADAR tool enables precise and efficient A-to-I RNA editing in a guideRNA-dependent manner by applying the self-labeling SNAP-tag enzyme to generate RNA-guided editases in cell culture. Here, we extend this platform by combining the SNAP-tagged tool with further effectors steered by the orthogonal HALO-tag. Due to their small size (ca. 2 kb), both effectors are readily integrated into one genomic locus. We demonstrate selective and concurrent recruitment of ADAR1 and ADAR2 deaminase activity for optimal editing with extended substrate scope and moderate global off-target effects. Furthermore, we combine the recruitment of ADAR1 and APOBEC1 deaminase activity to achieve selective and concurrent A-to-I and C-to-U RNA base editing of endogenous transcripts inside living cells, again with moderate global off-target effects. The platform should be readily transferable to further epitranscriptomic writers and erasers to manipulate epitranscriptomic marks in a programmable way with high molecular precision. | ||
| 700 | 1 | |a Latifi, Ngadhnjim |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hanswillemenke, Alfred |e VerfasserIn |4 aut | |
| 700 | 1 | |a Tasakis, Rafail Nikolaos |d 1994- |e VerfasserIn |0 (DE-588)1250522013 |0 (DE-627)1787391752 |4 aut | |
| 700 | 1 | |a Papavasiliou, Nina |e VerfasserIn |0 (DE-588)1234989549 |0 (DE-627)1760001279 |4 aut | |
| 700 | 1 | |a Stafforst, Thorsten |d 1978- |e VerfasserIn |0 (DE-588)132492067 |0 (DE-627)522964680 |0 (DE-576)299180603 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nucleic acids research |d Oxford : Oxford Univ. Press, 1974 |g 49(2021), 16, Artikel-ID e95, Seite 1-12 |h Online-Ressource |w (DE-627)26813250X |w (DE-600)1472175-2 |w (DE-576)07760878X |x 1362-4962 |7 nnas |a Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing |
| 773 | 1 | 8 | |g volume:49 |g year:2021 |g number:16 |g elocationid:e95 |g pages:1-12 |g extent:12 |a Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing |
| 856 | 4 | 0 | |u https://doi.org/10.1093/nar/gkab541 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211122 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1234989549 |a Papavasiliou, Nina |m 1234989549:Papavasiliou, Nina |d 50000 |e 50000PP1234989549 |k 0/50000/ |p 5 | ||
| 998 | |g 1250522013 |a Tasakis, Rafail Nikolaos |m 1250522013:Tasakis, Rafail Nikolaos |d 140000 |e 140000PT1250522013 |k 0/140000/ |p 4 | ||
| 999 | |a KXP-PPN1778218199 |e 4004557240 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing","title_sort":"Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editing"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"note":["Gesehen am 22.11.2021"],"origin":[{"dateIssuedDisp":"01 July 2021","dateIssuedKey":"2021"}],"person":[{"display":"Stroppel, Anna","role":"aut","given":"Anna","family":"Stroppel"},{"display":"Latifi, Ngadhnjim","role":"aut","given":"Ngadhnjim","family":"Latifi"},{"role":"aut","given":"Alfred","family":"Hanswillemenke","display":"Hanswillemenke, Alfred"},{"display":"Tasakis, Rafail Nikolaos","family":"Tasakis","given":"Rafail Nikolaos","role":"aut"},{"display":"Papavasiliou, Nina","family":"Papavasiliou","given":"Nina","role":"aut"},{"family":"Stafforst","role":"aut","given":"Thorsten","display":"Stafforst, Thorsten"}],"relHost":[{"id":{"eki":["26813250X"],"issn":["1362-4962"],"zdb":["1472175-2"]},"part":{"volume":"49","year":"2021","issue":"16","text":"49(2021), 16, Artikel-ID e95, Seite 1-12","extent":"12","pages":"1-12"},"pubHistory":["1.1974 -"],"titleAlt":[{"title":"NAR online"},{"title":"NAR"},{"title":"Database issue"},{"title":"Web server issue"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"note":["Gesehen am 15.09.2025","Ungezählte Beil.: Database issue; Web server issue"],"title":[{"title_sort":"Nucleic acids research","title":"Nucleic acids research"}],"origin":[{"publisher":"Oxford Univ. Press","dateIssuedKey":"1974","publisherPlace":"Oxford","dateIssuedDisp":"1974-"}],"disp":"Harnessing self-labeling enzymes for selective and concurrent A-to-I and C-to-U RNA base editingNucleic acids research","physDesc":[{"extent":"Online-Ressource"}],"recId":"26813250X"}],"recId":"1778218199","physDesc":[{"extent":"12 S."}],"id":{"doi":["10.1093/nar/gkab541"],"eki":["1778218199"]},"name":{"displayForm":["Anna S. Stroppel, Ngadhnjim Latifi, Alfred Hanswillemenke, Rafail Nikolaos Tasakis, F. Nina Papavasiliou and Thorsten Stafforst"]}} | ||
| SRT | |a STROPPELANHARNESSING0120 | ||