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Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states

Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high-content...

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Main Authors: Triana, Sergio (Author) , Vonficht, Dominik (Author) , Jopp-Saile, Lea (Author) , Raffel, Simon (Author) , Lutz, Raphael (Author) , Leonce, Daniel (Author) , Pajonk, Magdalena (Author) , Hernández-Malmierca, Pablo (Author) , Ordoñez-Rueda, Diana (Author) , Ramasz, Beáta (Author) , Boch, Tobias (Author) , Jann, Johann-Christoph (Author) , Nowak, Daniel (Author) , Hofmann, Wolf-Karsten (Author) , Müller-Tidow, Carsten (Author) , Hübschmann, Daniel (Author) , Alexandrov, Theodore (Author) , Benes, Vladimir (Author) , Trumpp, Andreas (Author) , Paulsen, Malte (Author) , Velten, Lars (Author) , Haas, Simon (Author)
Format: Article (Journal)
Language:English
Published: 22 November 2021
In: Nature immunology
Year: 2021, Volume: 22, Issue: 12, Pages: 1577-1589
ISSN:1529-2916
DOI:10.1038/s41590-021-01059-0
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41590-021-01059-0
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41590-021-01059-0
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Author Notes:Sergio Triana, Dominik Vonficht, Lea Jopp-Saile, Simon Raffel, Raphael Lutz, Daniel Leonce, Magdalena Antes, Pablo Hernández-Malmierca, Diana Ordoñez-Rueda, Beáta Ramasz, Tobias Boch, Johann-Christoph Jann, Daniel Nowak, Wolf-Karsten Hofmann, Carsten Müller-Tidow, Daniel Hübschmann, Theodore Alexandrov, Vladimir Benes, Andreas Trumpp, Malte Paulsen, Lars Velten and Simon Haas
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Summary:Single-cell genomics technology has transformed our understanding of complex cellular systems. However, excessive cost and a lack of strategies for the purification of newly identified cell types impede their functional characterization and large-scale profiling. Here, we have generated high-content single-cell proteo-genomic reference maps of human blood and bone marrow that quantitatively link the expression of up to 197 surface markers to cellular identities and biological processes across all main hematopoietic cell types in healthy aging and leukemia. These reference maps enable the automatic design of cost-effective high-throughput cytometry schemes that outperform state-of-the-art approaches, accurately reflect complex topologies of cellular systems and permit the purification of precisely defined cell states. The systematic integration of cytometry and proteo-genomic data enables the functional capacities of precisely mapped cell states to be measured at the single-cell level. Our study serves as an accessible resource and paves the way for a data-driven era in cytometry.
Item Description:Das PDF enthält einen Anhang von 26 Seiten
Gesehen am 01.12.2021
Physical Description:Online Resource
ISSN:1529-2916
DOI:10.1038/s41590-021-01059-0

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