Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach
Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conju...
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| Main Authors: | , , |
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| Format: | Article (Journal) Video |
| Language: | English |
| Published: |
2013
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| In: |
JoVE. Video journal
Year: 2013, Issue: 78, Pages: ? |
| ISSN: | 1940-087X |
| DOI: | 10.3791/50842 |
| Subjects: | |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/50842 Verlag, lizenzpflichtig, Volltext: https://www.jove.com/de/v/50842/covalent-binding-bmp-2-on-surfaces-using-self-assembled-monolayer 
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| Author Notes: | Theresa L.M. Pohl, Elisabeth H. Schwab, Elisabetta A. Cavalcanti-Adam |
| Summary: | Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conjunction with scaffolds has raised recent controversies, based on the mode of presentation and the amount to be delivered. The protocol presented here provides a simple and efficient way to deliver BMP-2 for in vitro studies on cells. We describe how to form a self-assembled monolayer consisting of a heterobifunctional linker, and show the subsequent binding step to obtain covalent immobilization of rhBMP-2. With this approach it is possible to achieve a sustained presentation of BMP-2 while maintaining the biological activity of the protein. In fact, the surface immobilization of BMP-2 allows targeted investigations by preventing unspecific adsorption, while reducing the amount of growth factor and, most notably, hindering uncontrolled release from the surface. Both short- and long-term signaling events triggered by BMP-2 are taking place when cells are exposed to surfaces presenting covalently immobilized rhBMP-2, making this approach suitable for in vitro studies on cell responses to BMP-2 stimulation. |
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| Item Description: | Enthält auch Textversion Gesehen am 03.12.2021 Wissenschaftlicher Film. Deutschland. 2013 |
| Physical Description: | Online Resource |
| ISSN: | 1940-087X |
| DOI: | 10.3791/50842 |