Cover Image

Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach

Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conju...

Full description

Saved in:
Bibliographic Details
Main Authors: Pohl, Theresa Lucie Maria (Author) , Baha-Schwab, Elisabeth H. (Author) , Cavalcanti-Adam, Elisabetta A. (Author)
Format: Article (Journal) Video
Language:English
Published: 2013
In: JoVE. Video journal
Year: 2013, Issue: 78, Pages: ?
ISSN:1940-087X
DOI:10.3791/50842
Subjects:
Film
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3791/50842
Verlag, lizenzpflichtig, Volltext: https://www.jove.com/de/v/50842/covalent-binding-bmp-2-on-surfaces-using-self-assembled-monolayer
Get full text
Author Notes:Theresa L.M. Pohl, Elisabeth H. Schwab, Elisabetta A. Cavalcanti-Adam

MARC

LEADER 00000cga a2200000 c 4500
001 1780187114
003 DE-627
005 20230621152101.0
006 m o | |
007 cr uuu---uuuuu
008 211203s2013 xx ||| | o ||eng c
024 7 |a 10.3791/50842  |2 doi 
035 |a (DE-627)1780187114 
035 |a (DE-599)KXP1780187114 
035 |a (OCoLC)1341434473 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 29  |2 sdnb 
100 1 |a Pohl, Theresa Lucie Maria  |e VerfasserIn  |0 (DE-588)1032361212  |0 (DE-627)738376426  |0 (DE-576)379994992  |4 aut 
245 1 0 |a Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach  |c Theresa L.M. Pohl, Elisabeth H. Schwab, Elisabetta A. Cavalcanti-Adam 
264 1 |c 2013 
300 |a 1 Online-Ressource (1 Videodatei, 2:00 min)  |b farbig 
336 |a Text  |b txt  |2 rdacontent 
336 |a zweidimensionales bewegtes Bild  |b tdi  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Enthält auch Textversion 
500 |a Gesehen am 03.12.2021 
500 |a Wissenschaftlicher Film. Deutschland. 2013 
520 |a Bone morphogenetic protein 2 (BMP-2) is a growth factor embedded in the extracellular matrix of bone tissue. BMP-2 acts as trigger of mesenchymal cell differentiation into osteoblasts, thus stimulating healing and de novo bone formation. The clinical use of recombinant human BMP-2 (rhBMP-2) in conjunction with scaffolds has raised recent controversies, based on the mode of presentation and the amount to be delivered. The protocol presented here provides a simple and efficient way to deliver BMP-2 for in vitro studies on cells. We describe how to form a self-assembled monolayer consisting of a heterobifunctional linker, and show the subsequent binding step to obtain covalent immobilization of rhBMP-2. With this approach it is possible to achieve a sustained presentation of BMP-2 while maintaining the biological activity of the protein. In fact, the surface immobilization of BMP-2 allows targeted investigations by preventing unspecific adsorption, while reducing the amount of growth factor and, most notably, hindering uncontrolled release from the surface. Both short- and long-term signaling events triggered by BMP-2 are taking place when cells are exposed to surfaces presenting covalently immobilized rhBMP-2, making this approach suitable for in vitro studies on cell responses to BMP-2 stimulation. 
655 7 |a Film  |0 (DE-588)4017102-4  |0 (DE-627)104559683  |0 (DE-576)208918531  |2 gnd-content 
700 1 |a Baha-Schwab, Elisabeth H.  |e VerfasserIn  |0 (DE-588)1059178958  |0 (DE-627)798131292  |0 (DE-576)415200350  |4 aut 
700 1 |a Cavalcanti-Adam, Elisabetta A.  |d 1972-  |e VerfasserIn  |0 (DE-588)1059179458  |0 (DE-627)798132256  |0 (DE-576)189133511  |4 aut 
773 0 8 |i Enthalten in  |t JoVE. Video journal  |d [Erscheinungsort nicht ermittelbar] : [Verlag nicht ermittelbar], 2006  |g (2013), 78, Artikel-ID e50842, Seite ?  |h Online-Ressource  |w (DE-627)521454778  |w (DE-600)2259946-0  |w (DE-576)338522980  |x 1940-087X  |7 nngs  |a Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach 
773 1 8 |g year:2013  |g number:78  |g elocationid:e50842  |g pages:?  |a Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach 
856 4 0 |u https://doi.org/10.3791/50842  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.jove.com/de/v/50842/covalent-binding-bmp-2-on-surfaces-using-self-assembled-monolayer  |x Verlag  |z lizenzpflichtig  |3 Volltext 
935 |c vide 
937 |a Wissenschaftlicher Film  |b Deutschland  |c 2013 
951 |a AR 
992 |a 20211203 
993 |a Video 
994 |a 2013 
998 |g 1032361212  |a Pohl, Theresa Lucie Maria  |m 1032361212:Pohl, Theresa Lucie Maria  |p 1  |x j 
998 |g 1059179458  |a Cavalcanti-Adam, Elisabetta A.  |m 1059179458:Cavalcanti-Adam, Elisabetta A.  |d 120000  |e 120000PC1059179458  |k 0/120000/  |p 3  |y j 
998 |g 1059178958  |a Baha-Schwab, Elisabeth H.  |m 1059178958:Baha-Schwab, Elisabeth H.  |d 120000  |d 120300  |e 120000PB1059178958  |e 120300PB1059178958  |k 0/120000/  |k 1/120000/120300/  |p 2 
999 |a KXP-PPN1780187114  |e 4013446332 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Pohl, Theresa Lucie Maria","given":"Theresa Lucie Maria","family":"Pohl"},{"family":"Baha-Schwab","given":"Elisabeth H.","display":"Baha-Schwab, Elisabeth H.","roleDisplay":"VerfasserIn","role":"aut"},{"role":"aut","display":"Cavalcanti-Adam, Elisabetta A.","roleDisplay":"VerfasserIn","given":"Elisabetta A.","family":"Cavalcanti-Adam"}],"title":[{"title":"Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach","title_sort":"Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Enthält auch Textversion","Gesehen am 03.12.2021","Wissenschaftlicher Film. Deutschland. 2013"],"language":["eng"],"recId":"1780187114","name":{"displayForm":["Theresa L.M. Pohl, Elisabeth H. Schwab, Elisabetta A. Cavalcanti-Adam"]},"origin":[{"dateIssuedDisp":"2013","dateIssuedKey":"2013"}],"id":{"doi":["10.3791/50842"],"eki":["1780187114"]},"physDesc":[{"noteIll":"farbig","extent":"1 Online-Ressource (1 Videodatei, 2:00 min) S."}],"relHost":[{"title":[{"title_sort":"JoVE","title":"JoVE","partname":"Video journal"}],"note":["Gesehen am 10.02.25","Inhaltliche Gliederung in: Behavior; Biochemistry; Bioengineering; Biology; Cancer research; Chemistry; Developmental biology; Engineering; Environment; Genetics; Immunology and infection; Medicine; Neuroscience"],"disp":"Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approachJoVE. Video journal","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"521454778","language":["eng"],"pubHistory":["1.2006 -"],"titleAlt":[{"title":"JoVE journal"},{"title":"JoVE journal Biology"},{"title":"JoVE journal Medicine"},{"title":"JoVE journal Behavior"},{"title":"JoVE journal Biochemistry"},{"title":"JoVE journal Bioengineering"},{"title":"JoVE journal Cancer research"},{"title":"JoVE journal Chemistry"},{"title":"JoVE journal Developmental biology"},{"title":"JoVE journal Engineering"},{"title":"JoVE journal Environment"},{"title":"JoVE journal Genetics"},{"title":"JoVE journal Immunology and infection"},{"title":"JoVE journal Neuroscience"},{"title":"Journal of visualized experiments"}],"part":{"text":"(2013), 78, Artikel-ID e50842, Seite ?","year":"2013","pages":"?","issue":"78"},"origin":[{"publisherPlace":"[Erscheinungsort nicht ermittelbar]","dateIssuedDisp":"2006-","publisher":"[Verlag nicht ermittelbar]","dateIssuedKey":"2006"}],"id":{"issn":["1940-087X"],"zdb":["2259946-0"],"eki":["521454778"]},"physDesc":[{"extent":"Online-Ressource"}]}]} 
SRT |a POHLTHERESCOVALENTBI2013 

Similar Items