rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Hematopoietic progenitor cells and cellular microenvironment
    sub title: behavioral and molecular changes upon interaction
  name:
    name part: Wagner, Wolfgang
    name part: 1973-
    role:
      role term: creator
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Saffrich, Rainer
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Wirkner, Ute
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Eckstein, Volker
    name part: 1957-
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Blake, Jonathon
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Ansorge, Alexandra
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Schwager, Christian
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Wein, Frederik
    name part: 1976-
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Miesala, Katrin
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Ansorge, Wilhelm
    name part: 1944-
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Ho, Anthony Dick
    name part: 1948-
    role:
      role term: VerfasserIn
    role:
      role term: aut
  type of resource: text
  origin info:
    place:
      place term: xx
    date issued: 2005
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 12
  abstract: Cell-cell contact between stem cells and cellular determinants of the microenvironment plays an essential role in controlling cell division. Using human hematopoietic progenitor cells (CD34+/CD38−) and a stroma cell line (AFT024) as a model, we have studied the initial behavioral and molecular sequel of this interaction. Time-lapse microscopy showed that CD34+/CD38− cells actively migrated toward and sought contact with stroma cells and 30% of them adhered firmly to AFT024 stroma through the uropod. CD44 and CD34 are colocalized at the site of contact. Gene expression profiles of CD34+/CD38− cells upon cultivation with or without stroma for 16, 20, 48, or 72 hours were analyzed using our human genome cDNA microarray. Chk1, egr1, and cxcl2 were among the first genes upregulated within 16 hours. Genes with the highest upregulation throughout the time course included tubulin genes, ezrin, c1qr1, fos, pcna, mcm6, ung, and dnmt1, genes that play an essential role in reorganization of the cytoskeleton system, stabilization of DNA, and methylation patterns. Our results demonstrate directed migration of CD34+/CD38− cells toward AFT024 and adhesion through the uropod and that upon interaction with supportive stroma, reorganization of the cytoskeleton system, regulation of cell division, and maintenance of genetic stability represent the most essential steps.
  note: Wolfgang Wagner, Rainer Saffrich, Ute Wirkner, Volker Eckstein, Jonathon Blake, Alexandra Ansorge, Christian Schwager, Frederik Wein, Katrin Miesala, Wilhelm Ansorge, Anthony D. Ho
  note: Elektronische Reproduktion der Druck-Ausgabe
  note: Gesehen am 03.12.2021
  subject:
    topic: Adhesion
  subject:
    topic: Hematopoietic stem cell
  subject:
    topic: Microarray
  subject:
    topic: Microenvironment
  subject:
    topic: Uropod
  classification: 33
  related item:
    title info:
      title: Stem cells
    name:
      name part: Hematopoietic progenitor cells and cellular microenvironment behavioral and molecular changes upon interaction
    origin info:
      publisher: Oxford : Oxford University Press, 1993
    physical description:
      extent: Online-Ressource
    identifier: 1549-4918
    identifier: (DE-627)324746296
    identifier: (DE-600)2030643-X
    identifier: (DE-576)106890816
    part:
      text: 23(2005), 8, Seite 1180-1191
  related item:
    name:
      name part: Hematopoietic progenitor cells and cellular microenvironment behavioral and molecular changes upon interaction
    part:
      text: volume:23
    part:
      text: year:2005
    part:
      text: number:8
    part:
      text: pages:1180-1191
    part:
      text: extent:12
  identifier: https://doi.org/10.1634/stemcells.2004-0361
  identifier: https://onlinelibrary.wiley.com/doi/abs/10.1634/stemcells.2004-0361
  record info:
    record content source: DE-627
    record creation date: 211203
    record change date: 20220820085412.0
    record identifier: 1780190824
    language of cataloging:
      language term: ger