Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE
The receptor protein tyrosine phosphatase alpha (PTPα) is involved in the regulation of tyrosine kinases like the Src kinase and the insulin receptor. As with other PTPs, its function is determined by alternative splicing, dimerisation, phosphorylation and proteolytical processing. PTPα is cleaved b...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
29 May 2012
|
| In: |
European journal of cell biology
Year: 2012, Volume: 91, Issue: 9, Pages: 687-693 |
| ISSN: | 1618-1298 |
| DOI: | 10.1016/j.ejcb.2012.04.003 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ejcb.2012.04.003
|
| Author Notes: | Katja Kapp, Jan Siemens, Hans-Ulrich Häring, Reiner Lammers |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1780381964 | ||
| 003 | DE-627 | ||
| 005 | 20220820090039.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211206s2012 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ejcb.2012.04.003 |2 doi | |
| 035 | |a (DE-627)1780381964 | ||
| 035 | |a (DE-599)KXP1780381964 | ||
| 035 | |a (OCoLC)1341434725 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Kapp, Katja |e VerfasserIn |0 (DE-588)1246978334 |0 (DE-627)1780382707 |4 aut | |
| 245 | 1 | 0 | |a Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE |c Katja Kapp, Jan Siemens, Hans-Ulrich Häring, Reiner Lammers |
| 264 | 1 | |c 29 May 2012 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 06.12.2021 | ||
| 520 | |a The receptor protein tyrosine phosphatase alpha (PTPα) is involved in the regulation of tyrosine kinases like the Src kinase and the insulin receptor. As with other PTPs, its function is determined by alternative splicing, dimerisation, phosphorylation and proteolytical processing. PTPα is cleaved by calpain in its intracellular domain, which decreases its potential to dephosphorylate Src kinase. Here, we demonstrate that PTPα is also processed in the extracellular domain. Extracellular processing was exclusively found for a splice variant containing an extra nine amino acid insert three residues amino-terminal from the transmembrane domain. Processing was sensitive to the metalloprotease-inhibitor Batimastat, and CHO-M2 cells lacking a disintegrin and metalloproteinase 17 (ADAM17; tumor-necrosis-factor α converting enzyme) activity were not able to cleave PTPα. After transient overexpression of ADAM17 and PTPα in these cells, processing was restored, proving that ADAM17 is involved in this process. Further characterization of the consequences of processing revealed that dephosphorylation of the insulin receptor or activation of Src was not affected but focus formation was reduced. We conclude that extracellular proteolytic processing is a novel mechanism for PTPα regulation. | ||
| 650 | 4 | |a ADAM Proteins | |
| 650 | 4 | |a ADAM17 Protein | |
| 650 | 4 | |a Animals | |
| 650 | 4 | |a Cell Line | |
| 650 | 4 | |a CHO Cells | |
| 650 | 4 | |a Cricetinae | |
| 650 | 4 | |a HEK293 Cells | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Mice | |
| 650 | 4 | |a NIH 3T3 Cells | |
| 650 | 4 | |a Protein Structure, Tertiary | |
| 650 | 4 | |a Protein Tyrosine Phosphatases | |
| 650 | 4 | |a Proteolysis | |
| 700 | 1 | |a Siemens, Jan |e VerfasserIn |0 (DE-588)1067334610 |0 (DE-627)818630752 |0 (DE-576)426567323 |4 aut | |
| 700 | 1 | |a Häring, Hans-Ulrich |d 1951- |e VerfasserIn |0 (DE-588)143497979 |0 (DE-627)646578537 |0 (DE-576)172395356 |4 aut | |
| 700 | 1 | |a Lammers, Reiner |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European journal of cell biology |d München : Elsevier, 1998 |g 91(2012), 9, Seite 687-693 |h Online-Ressource |w (DE-627)334288150 |w (DE-600)2056855-1 |w (DE-576)094661901 |x 1618-1298 |7 nnas |a Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE |
| 773 | 1 | 8 | |g volume:91 |g year:2012 |g number:9 |g pages:687-693 |g extent:7 |a Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.ejcb.2012.04.003 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211206 | ||
| 993 | |a Article | ||
| 994 | |a 2012 | ||
| 998 | |g 1067334610 |a Siemens, Jan |m 1067334610:Siemens, Jan |p 2 | ||
| 999 | |a KXP-PPN1780381964 |e 4014539601 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"pubHistory":["Nachgewiesen 75.1998 -"],"titleAlt":[{"title":"EJCB"}],"part":{"extent":"7","volume":"91","text":"91(2012), 9, Seite 687-693","pages":"687-693","issue":"9","year":"2012"},"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACEEuropean journal of cell biology","note":["Fortsetzung der Druck-Ausgabe","Gesehen am 11.05.2021"],"language":["eng"],"recId":"334288150","title":[{"title_sort":"European journal of cell biology","title":"European journal of cell biology"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedKey":"1998","publisher":"Elsevier ; Urban & Fischer","dateIssuedDisp":"1998-","publisherPlace":"München ; Jena"}],"id":{"issn":["1618-1298"],"zdb":["2056855-1"],"eki":["334288150"]}}],"physDesc":[{"extent":"7 S."}],"name":{"displayForm":["Katja Kapp, Jan Siemens, Hans-Ulrich Häring, Reiner Lammers"]},"id":{"eki":["1780381964"],"doi":["10.1016/j.ejcb.2012.04.003"]},"origin":[{"dateIssuedDisp":"29 May 2012","dateIssuedKey":"2012"}],"language":["eng"],"recId":"1780381964","type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 06.12.2021"],"person":[{"roleDisplay":"VerfasserIn","display":"Kapp, Katja","role":"aut","family":"Kapp","given":"Katja"},{"display":"Siemens, Jan","roleDisplay":"VerfasserIn","role":"aut","family":"Siemens","given":"Jan"},{"roleDisplay":"VerfasserIn","display":"Häring, Hans-Ulrich","role":"aut","family":"Häring","given":"Hans-Ulrich"},{"roleDisplay":"VerfasserIn","display":"Lammers, Reiner","role":"aut","family":"Lammers","given":"Reiner"}],"title":[{"title_sort":"Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE","title":"Proteolytic processing of the protein tyrosine phosphatase α extracellular domain is mediated by ADAM17/TACE"}]} | ||
| SRT | |a KAPPKATJASPROTEOLYTI2920 | ||