Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells
High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling indu...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 28, 2021
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| In: |
Cell reports. Methods
Year: 2021, Jahrgang: 1, Heft: 7, Pages: 1-13, e1-3 |
| ISSN: | 2667-2375 |
| DOI: | 10.1016/j.crmeth.2021.100117 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.crmeth.2021.100117 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2667237521001818 
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| Verfasserangaben: | Valentina Loconte, Jian-Hua Chen, Mirko Cortese, Axel Ekman, Mark A. Le Gros, Carolyn Larabell, Ralf Bartenschlager, and Venera Weinhardt |
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| 520 | |a High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution and throughput. Most of the current SXT systems suffer from a restricted field of view due to use of flat sample supports and artifacts due to missing data. In this approach using cylindrical sample holders, a full-rotation tomogram of human lung epithelial cells is performed in less than 10 min. We demonstrate the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner. | ||
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