Role of Rck-Pat1b binding in assembly of processing-bodies
The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping. In addition, both proteins are required for the assembly of processing (P)-bodies, cytoplasmic foci...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
27 Mar 2013
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| In: |
RNA biology
Year: 2013, Volume: 10, Issue: 4, Pages: 528-539 |
| ISSN: | 1555-8584 |
| DOI: | 10.4161/rna.24086 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.4161/rna.24086
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| Author Notes: | Sevim Ozgur & Georg Stoecklin |
| Summary: | The DEAD box RNA helicase Rck and the scaffold protein Pat1b participate in controlling gene expression at the post-transcriptional level by suppressing mRNA translation and promoting mRNA decapping. In addition, both proteins are required for the assembly of processing (P)-bodies, cytoplasmic foci that contain stalled mRNAs and numerous components of the mRNA decay machinery. The C-terminal RecA-like domain of Rck interacts with the N-terminal acidic domain of Pat1b. Here, we identified point mutations in human Rck and Pat1b that prevent the two proteins from binding to each other. By analyzing interaction-deficient mutants in combination with knockdown and rescue strategies in human HeLa cells, we found that Pat1b assembles P-bodies and suppresses expression of tethered mRNAs in the absence of Rck binding. In contrast, Rck requires the Pat1b-binding site in order to promote P-body assembly and associate with the decapping enzyme Dcp2 as well as Ago2 and TNRC6A, two core components of the RNA-induced silencing complex. Our data indicate that P-body assembly occurs in a step-wise manner, where Rck participates in the initial suppression of mRNA translation, whereas Pat1b in a second step triggers P-body assembly and promotes mRNA decapping. |
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| Item Description: | Gesehen am 13.12.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1555-8584 |
| DOI: | 10.4161/rna.24086 |