XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase
Replication-associated single-ended DNA double-strand breaks (seDSBs) are repaired predominantly through RAD51-mediated homologous recombination (HR). Removal of the non-homologous end-joining (NHEJ) factor Ku from resected seDSB ends is crucial for HR. The coordinated actions of MRE11-CtIP nuclease...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
09 September 2021
|
| In: |
Nucleic acids research
Year: 2021, Jahrgang: 49, Heft: 17, Pages: 9906-9925 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkab785 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gkab785
|
| Verfasserangaben: | Abhishek Bharadwaj Sharma, Hélène Erasimus, Lia Pinto, Marie-Christine Caron, Diyavarshini Gopaul, Thibaut Peterlini, Katrin Neumann, Petr V. Nazarov, Sabrina Fritah, Barbara Klink, Christel C. Herold-Mende, Simone P. Niclou, Philippe Pasero, Patrick Calsou, Jean-Yves Masson, Sébastien Britton, Eric Van Dyck |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1782234802 | ||
| 003 | DE-627 | ||
| 005 | 20220820094225.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 211215s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nar/gkab785 |2 doi | |
| 035 | |a (DE-627)1782234802 | ||
| 035 | |a (DE-599)KXP1782234802 | ||
| 035 | |a (OCoLC)1341436320 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Sharma, Abhishek |e VerfasserIn |0 (DE-588)1127362305 |0 (DE-627)881873527 |0 (DE-576)485204827 |4 aut | |
| 245 | 1 | 0 | |a XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase |c Abhishek Bharadwaj Sharma, Hélène Erasimus, Lia Pinto, Marie-Christine Caron, Diyavarshini Gopaul, Thibaut Peterlini, Katrin Neumann, Petr V. Nazarov, Sabrina Fritah, Barbara Klink, Christel C. Herold-Mende, Simone P. Niclou, Philippe Pasero, Patrick Calsou, Jean-Yves Masson, Sébastien Britton, Eric Van Dyck |
| 246 | 3 | 0 | |a two |
| 264 | 1 | |c 09 September 2021 | |
| 300 | |a 20 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 15.12.2021 | ||
| 520 | |a Replication-associated single-ended DNA double-strand breaks (seDSBs) are repaired predominantly through RAD51-mediated homologous recombination (HR). Removal of the non-homologous end-joining (NHEJ) factor Ku from resected seDSB ends is crucial for HR. The coordinated actions of MRE11-CtIP nuclease activities orchestrated by ATM define one pathway for Ku eviction. Here, we identify the pre-mRNA splicing protein XAB2 as a factor required for resistance to seDSBs induced by the chemotherapeutic alkylator temozolomide. Moreover, we show that XAB2 prevents Ku retention and abortive HR at seDSBs induced by temozolomide and camptothecin, via a pathway that operates in parallel to the ATM-CtIP-MRE11 axis. Although XAB2 depletion preserved RAD51 focus formation, the resulting RAD51-ssDNA associations were unproductive, leading to increased NHEJ engagement in S/G2 and genetic instability. Overexpression of RAD51 or RAD52 rescued the XAB2 defects and XAB2 loss was synthetically lethal with RAD52 inhibition, providing potential perspectives in cancer therapy. | ||
| 700 | 1 | |a Erasimus, Hélène |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pinto, Lia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Caron, Marie-Christine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gopaul, Diyavarshini |e VerfasserIn |4 aut | |
| 700 | 1 | |a Peterlini, Thibaut |e VerfasserIn |4 aut | |
| 700 | 1 | |a Neumann, Katrin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nazarov, Petr V |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fritah, Sabrina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Klink, Barbara |e VerfasserIn |4 aut | |
| 700 | 1 | |a Herold-Mende, Christel |e VerfasserIn |0 (DE-588)1022936549 |0 (DE-627)717335577 |0 (DE-576)366194267 |4 aut | |
| 700 | 1 | |a Niclou, Simone P |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pasero, Philippe |e VerfasserIn |4 aut | |
| 700 | 1 | |a Calsou, Patrick |e VerfasserIn |4 aut | |
| 700 | 1 | |a Masson, Jean-Yves |e VerfasserIn |4 aut | |
| 700 | 1 | |a Britton, Sébastien |e VerfasserIn |4 aut | |
| 700 | 1 | |a Van Dyck, Eric |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nucleic acids research |d Oxford : Oxford Univ. Press, 1974 |g 49(2021), 17, Seite 9906-9925 |h Online-Ressource |w (DE-627)26813250X |w (DE-600)1472175-2 |w (DE-576)07760878X |x 1362-4962 |7 nnas |a XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase |
| 773 | 1 | 8 | |g volume:49 |g year:2021 |g number:17 |g pages:9906-9925 |g extent:20 |a XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase |
| 856 | 4 | 0 | |u https://doi.org/10.1093/nar/gkab785 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20211215 | ||
| 993 | |a Article | ||
| 994 | |a 2021 | ||
| 998 | |g 1022936549 |a Herold-Mende, Christel |m 1022936549:Herold-Mende, Christel |d 910000 |d 911200 |e 910000PH1022936549 |e 911200PH1022936549 |k 0/910000/ |k 1/910000/911200/ |p 11 | ||
| 999 | |a KXP-PPN1782234802 |e 4020439893 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase","title":"XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase"}],"language":["eng"],"person":[{"given":"Abhishek","role":"aut","display":"Sharma, Abhishek","family":"Sharma"},{"display":"Erasimus, Hélène","given":"Hélène","role":"aut","family":"Erasimus"},{"given":"Lia","role":"aut","display":"Pinto, Lia","family":"Pinto"},{"display":"Caron, Marie-Christine","given":"Marie-Christine","role":"aut","family":"Caron"},{"family":"Gopaul","role":"aut","given":"Diyavarshini","display":"Gopaul, Diyavarshini"},{"role":"aut","given":"Thibaut","display":"Peterlini, Thibaut","family":"Peterlini"},{"family":"Neumann","given":"Katrin","role":"aut","display":"Neumann, Katrin"},{"display":"Nazarov, Petr V","given":"Petr V","role":"aut","family":"Nazarov"},{"given":"Sabrina","role":"aut","display":"Fritah, Sabrina","family":"Fritah"},{"role":"aut","given":"Barbara","display":"Klink, Barbara","family":"Klink"},{"display":"Herold-Mende, Christel","given":"Christel","role":"aut","family":"Herold-Mende"},{"family":"Niclou","display":"Niclou, Simone P","role":"aut","given":"Simone P"},{"display":"Pasero, Philippe","given":"Philippe","role":"aut","family":"Pasero"},{"role":"aut","given":"Patrick","display":"Calsou, Patrick","family":"Calsou"},{"family":"Masson","given":"Jean-Yves","role":"aut","display":"Masson, Jean-Yves"},{"family":"Britton","role":"aut","given":"Sébastien","display":"Britton, Sébastien"},{"family":"Van Dyck","given":"Eric","role":"aut","display":"Van Dyck, Eric"}],"name":{"displayForm":["Abhishek Bharadwaj Sharma, Hélène Erasimus, Lia Pinto, Marie-Christine Caron, Diyavarshini Gopaul, Thibaut Peterlini, Katrin Neumann, Petr V. Nazarov, Sabrina Fritah, Barbara Klink, Christel C. Herold-Mende, Simone P. Niclou, Philippe Pasero, Patrick Calsou, Jean-Yves Masson, Sébastien Britton, Eric Van Dyck"]},"id":{"eki":["1782234802"],"doi":["10.1093/nar/gkab785"]},"physDesc":[{"extent":"20 S."}],"note":["Gesehen am 15.12.2021"],"relHost":[{"part":{"extent":"20","pages":"9906-9925","issue":"17","year":"2021","text":"49(2021), 17, Seite 9906-9925","volume":"49"},"language":["eng"],"title":[{"title":"Nucleic acids research","title_sort":"Nucleic acids research"}],"pubHistory":["1.1974 -"],"note":["Gesehen am 15.09.2025","Ungezählte Beil.: Database issue; Web server issue"],"disp":"XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinaseNucleic acids research","id":{"eki":["26813250X"],"zdb":["1472175-2"],"issn":["1362-4962"]},"physDesc":[{"extent":"Online-Ressource"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"titleAlt":[{"title":"NAR online"},{"title":"NAR"},{"title":"Database issue"},{"title":"Web server issue"}],"origin":[{"publisherPlace":"Oxford","dateIssuedKey":"1974","dateIssuedDisp":"1974-","publisher":"Oxford Univ. Press"}],"recId":"26813250X"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedDisp":"09 September 2021","dateIssuedKey":"2021"}],"recId":"1782234802"} | ||
| SRT | |a SHARMAABHIXAB2PROMOT0920 | ||