Lack of evidence of human papillomavirus-induced squamous cell carcinomas of the oral cavity in southern Germany
Objectives - The aim of the present study was to identify HPV-attributable SCC of the oral cavity (OSCC) in a cohort of patients from southern Germany. - Materials and methods - A sensitive PCR-enzyme immunoassay (EIA) was followed by a more specific in situ hybridization (ISH) to detect high risk h...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
19 April 2013
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| In: |
Oral oncology
Year: 2013, Volume: 49, Issue: 9, Pages: 937-942 |
| ISSN: | 1879-0593 |
| DOI: | 10.1016/j.oraloncology.2013.03.451 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.oraloncology.2013.03.451 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1368837513005356 
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| Author Notes: | Miriam Reuschenbach, Katinka Kansy, Kira Garbe, Svetlana Vinokurova, Christa Flechtenmacher, Csaba Toth, Elena-Sophie Prigge, Oliver C. Thiele, Siegmar Reinert, Jürgen Hoffmann, Magnus von Knebel Doeberitz, Kolja Freier |
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| 245 | 1 | 0 | |a Lack of evidence of human papillomavirus-induced squamous cell carcinomas of the oral cavity in southern Germany |c Miriam Reuschenbach, Katinka Kansy, Kira Garbe, Svetlana Vinokurova, Christa Flechtenmacher, Csaba Toth, Elena-Sophie Prigge, Oliver C. Thiele, Siegmar Reinert, Jürgen Hoffmann, Magnus von Knebel Doeberitz, Kolja Freier |
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| 520 | |a Objectives - The aim of the present study was to identify HPV-attributable SCC of the oral cavity (OSCC) in a cohort of patients from southern Germany. - Materials and methods - A sensitive PCR-enzyme immunoassay (EIA) was followed by a more specific in situ hybridization (ISH) to detect high risk human papillomavirus (HPV). An immunohistochemical dual-staining for p16INK4a and the proliferation marker Ki-67 was used to assess whether co-expression of p16INK4a/Ki-67 is a better surrogate marker for HPV in OSCC than p16INK4a alone, based on the hypothesis that combined p16INK4a and Ki-67 expression might specifically discriminate oncogene-induced p16INK4a expression from cell-cycle arrest-inducing senescence-associated p16INK4a expression. - Results - HPV-DNA by PCR-EIA could be detected in 25.1% (69/275) of the tumors, but ISH was negative in all of them. Diffuse p16INK4a overexpression was detected in 11 HPV PCR-positive tumors, but also in 6 HPV PCR-negative tumors. p16INK4a-expressing cells in diffusely positive tumors co-expressed Ki-67, irrespective of the HPV status. Neither the sole HPV status nor combined HPV/p16INK4a status nor the sole p16INK4a status was significantly associated with disease free or overall survival, however a trend towards better overall survival of patients whose tumor expressed p16INK4a in a focal pattern (=p16INK4a-positive/Ki-67-negative cells) compared to no p16INK4a expression (p=0.09) was observed. - Conclusion - Viral DNA can be detected in some tumors by a sensitive PCR, but absence of ISH signals indicates that the HPV-attributable fraction is smaller than estimated from PCR positivity. p16INK4a/Ki-67 co-expression is detectable in a fraction of OSCC irrespective of the HPV status. | ||
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