Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms
Algorithms that account for and overcome the intrinsic pixel-dependent readout noise from scientific complementary metal-oxide semiconductor (sCMOS) cameras prevent localization artifacts and avoid substantial compromises in localization uncertainty in single-molecule imaging. When combined with mul...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
26 May 2013
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| In: |
Nature methods
Year: 2013, Volume: 10, Issue: 7, Pages: 653-658 |
| ISSN: | 1548-7105 |
| DOI: | 10.1038/nmeth.2488 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nmeth.2488 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nmeth.2488 |
| Author Notes: | Fang Huang, Tobias MP Hartwich, Felix E Rivera-Molina, Yu Lin, Whitney C Duim, Jane J Long, Pradeep D Uchil, Jordan R Myers, Michelle A Baird, Walther Mothes, Michael W Davidson, Derek Toomre & Joerg Bewersdorf |
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| 520 | |a Algorithms that account for and overcome the intrinsic pixel-dependent readout noise from scientific complementary metal-oxide semiconductor (sCMOS) cameras prevent localization artifacts and avoid substantial compromises in localization uncertainty in single-molecule imaging. When combined with multi-emitter fitting, sCMOS cameras allow video-rate single-molecule switching nanoscopy. | ||
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