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NPM1 is overexpressed in hyperdiploid multiple myeloma due to a gain of chromosome 5 but is not delocalized to the cytoplasm

Multiple myeloma (MM) is proposed to consist of two main pathogenetic groups. Although hyperdiploid MM (HD) is characterized by multiple trisomies of odd chromosomes, in nonhyperdiploid MM (NHD), one of the recurrent primary immunoglobulin heavy chain (IGH) translocations and deletion of chromosome...

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Hauptverfasser: Weinhold, Niels (VerfasserIn) , Moreaux, Jérôme (VerfasserIn) , Raab, Marc-Steffen (VerfasserIn) , Hose, Dirk (VerfasserIn) , Hielscher, Thomas (VerfasserIn) , Benner, Axel (VerfasserIn) , Meißner, Tobias (VerfasserIn) , Ehrbrecht, Edith (VerfasserIn) , Brough, Michaela (VerfasserIn) , Jauch, Anna (VerfasserIn) , Goldschmidt, Hartmut (VerfasserIn) , Klein, Bernard (VerfasserIn) , Moos, Marion (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 13 January 2010
In: Genes, chromosomes & cancer
Year: 2010, Jahrgang: 49, Heft: 4, Pages: 333-341
ISSN:1098-2264
DOI:10.1002/gcc.20745
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/gcc.20745
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/gcc.20745
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Verfasserangaben:Niels Weinhold, Jerome Moreaux, Marc S. Raab, Dirk Hose, Thomas Hielscher, Axel Benner, Tobias Meißner, Edith Ehrbrecht, Michaela Brough, Anna Jauch, Hartmut Goldschmidt, Bernard Klein, and Marion Moos
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Zusammenfassung:Multiple myeloma (MM) is proposed to consist of two main pathogenetic groups. Although hyperdiploid MM (HD) is characterized by multiple trisomies of odd chromosomes, in nonhyperdiploid MM (NHD), one of the recurrent primary immunoglobulin heavy chain (IGH) translocations and deletion of chromosome 13 can frequently be found. In this study, we analyzed gene-expression profiles of patients with previously untreated MM. Fifty-four genes were significantly differentially expressed between the two groups. NPM1 was upregulated in HD. The differential expression of 25 genes, including NPM1 and 13 ribosomal protein genes, was validated using a published gene expression data set. The overexpression of NPM1 in HD was further confirmed by quantitative real-time PCR and Western blotting. NPM1 was significantly overexpressed in HD as the result of a gain of chromosome 5. Insertions into exon 12 of NPM1 were not detected. NPM1 was localized to the nucleoli of MM cells. Furthermore, HD was associated with an overexpression of ribosomal protein genes, independent of their localization on the trisomic or other chromosomes. Our results indicate that the gain of chromosome 5 might play an important role in the pathogenesis of HD. © 2010 Wiley-Liss, Inc.
Beschreibung:Gesehen am 07.01.2022
Beschreibung:Online Resource
ISSN:1098-2264
DOI:10.1002/gcc.20745

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