Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone
Employing antibodies against the cytoplasmic tail of the HIV-1 glycoprotein (Env-CT), in addition to gp160/gp41, we have identified several novel small Env proteins (<25kD) in HIV-1 transfected and infected cells. Mass spectrometric and mutational analyses show that two mechanisms contribute to t...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
3 April 2013
|
| In: |
Virology
Year: 2013, Volume: 441, Issue: 1, Pages: 85-94 |
| ISSN: | 1096-0341 |
| DOI: | 10.1016/j.virol.2013.03.009 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.virol.2013.03.009 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0042682213001499 
|
| Author Notes: | Tanya Pfeiffer, Thomas Ruppert, Heiner Schaal, Valerie Bosch |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1786598248 | ||
| 003 | DE-627 | ||
| 005 | 20230428185115.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220120s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.virol.2013.03.009 |2 doi | |
| 035 | |a (DE-627)1786598248 | ||
| 035 | |a (DE-599)KXP1786598248 | ||
| 035 | |a (OCoLC)1341438778 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Pfeiffer, Tanya |e VerfasserIn |0 (DE-588)1236244095 |0 (DE-627)1761475967 |4 aut | |
| 245 | 1 | 0 | |a Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone |c Tanya Pfeiffer, Thomas Ruppert, Heiner Schaal, Valerie Bosch |
| 264 | 1 | |c 3 April 2013 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 20.01.2022 | ||
| 520 | |a Employing antibodies against the cytoplasmic tail of the HIV-1 glycoprotein (Env-CT), in addition to gp160/gp41, we have identified several novel small Env proteins (<25kD) in HIV-1 transfected and infected cells. Mass spectrometric and mutational analyses show that two mechanisms contribute to their generation. Thus the protein, designated Tr-Env-CT (for truncated Env-CT), consists of the C-terminal 139 amino acids (aa) of Env (aa 718-856) with the N-terminal Q718 modified to pyroglutamic acid. It is likely derived from full-length Env protein by proteolytic processing. A further heterogeneous set of slightly larger proteins, termed Env-CT* species, are rather derived from spliced mRNAs containing only those Env C-terminal residues (aa 719-856) which overlap with the second tat and rev coding exons. They are N-terminally extended in the same reading frame. It is conceivable that essential Env-CT functions may be fulfilled by these novel species rather than by the full-length glycoprotein itself. | ||
| 650 | 4 | |a HIV Env C-terminus | |
| 650 | 4 | |a Novel Env-CT species | |
| 650 | 4 | |a Proteolytic processing | |
| 650 | 4 | |a Pyroglutamate modification | |
| 650 | 4 | |a Spliced mRNA encoding Env-CT | |
| 700 | 1 | |a Ruppert, Thomas |d 1973- |e VerfasserIn |0 (DE-588)124234895 |0 (DE-627)085725889 |0 (DE-576)294080147 |4 aut | |
| 700 | 1 | |a Schaal, Heiner |e VerfasserIn |0 (DE-588)173426999 |0 (DE-627)698341546 |0 (DE-576)134273133 |4 aut | |
| 700 | 1 | |a Bosch, Valerie |e VerfasserIn |0 (DE-588)1249959136 |0 (DE-627)1786600943 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Virology |d San Diego, Calif. [u.a.] : Elsevier, 1955 |g 441(2013), 1, Seite 85-94 |h Online-Ressource |w (DE-627)268130248 |w (DE-600)1471925-3 |w (DE-576)104194081 |x 1096-0341 |7 nnas |a Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone |
| 773 | 1 | 8 | |g volume:441 |g year:2013 |g number:1 |g pages:85-94 |g extent:10 |a Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.virol.2013.03.009 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0042682213001499 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220120 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1249959136 |a Bosch, Valerie |m 1249959136:Bosch, Valerie |d 50000 |e 50000PB1249959136 |k 0/50000/ |p 4 |y j | ||
| 998 | |g 124234895 |a Ruppert, Thomas |m 124234895:Ruppert, Thomas |d 700000 |d 706000 |e 700000PR124234895 |e 706000PR124234895 |k 0/700000/ |k 1/700000/706000/ |p 2 | ||
| 998 | |g 1236244095 |a Pfeiffer, Tanya |m 1236244095:Pfeiffer, Tanya |d 50000 |e 50000PP1236244095 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1786598248 |e 403981083X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1096-0341"],"eki":["268130248"],"zdb":["1471925-3"]},"origin":[{"publisherPlace":"San Diego, Calif. [u.a.] ; Orlando, Fla.","publisher":"Elsevier ; Academic Press","dateIssuedKey":"1955","dateIssuedDisp":"1955-"}],"recId":"268130248","language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 12.08.20"],"disp":"Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain aloneVirology","part":{"year":"2013","pages":"85-94","issue":"1","volume":"441","text":"441(2013), 1, Seite 85-94","extent":"10"},"pubHistory":["1.1955 -"],"title":[{"title":"Virology","title_sort":"Virology"}]}],"physDesc":[{"extent":"10 S."}],"id":{"doi":["10.1016/j.virol.2013.03.009"],"eki":["1786598248"]},"origin":[{"dateIssuedDisp":"3 April 2013","dateIssuedKey":"2013"}],"name":{"displayForm":["Tanya Pfeiffer, Thomas Ruppert, Heiner Schaal, Valerie Bosch"]},"recId":"1786598248","language":["eng"],"note":["Gesehen am 20.01.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone","title":"Detection and initial characterization of protein entities consisting of the HIV glycoprotein cytoplasmic C-terminal domain alone"}],"person":[{"role":"aut","display":"Pfeiffer, Tanya","roleDisplay":"VerfasserIn","given":"Tanya","family":"Pfeiffer"},{"display":"Ruppert, Thomas","roleDisplay":"VerfasserIn","role":"aut","family":"Ruppert","given":"Thomas"},{"role":"aut","display":"Schaal, Heiner","roleDisplay":"VerfasserIn","given":"Heiner","family":"Schaal"},{"display":"Bosch, Valerie","roleDisplay":"VerfasserIn","role":"aut","family":"Bosch","given":"Valerie"}]} | ||
| SRT | |a PFEIFFERTADETECTIONA3201 | ||