Watch out for reporter gene assays with Renilla luciferase and paclitaxel
Luminescence-based reporter gene assays are widely used in biochemistry. Signals from reporter genes (e.g., firefly luminescence) are usually normalized to signals from constantly luminescing luciferases such as Renilla luciferase. This normalization step can be performed by modern luminometry devic...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
7 March 2013
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| In: |
Analytical biochemistry
Year: 2013, Volume: 437, Issue: 2, Pages: 109-110 |
| ISSN: | 1096-0309 |
| DOI: | 10.1016/j.ab.2013.02.026 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ab.2013.02.026 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0003269713001073 
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| Author Notes: | Dirk Theile, Adriana Spalwisz, Johanna Weiss |
| Summary: | Luminescence-based reporter gene assays are widely used in biochemistry. Signals from reporter genes (e.g., firefly luminescence) are usually normalized to signals from constantly luminescing luciferases such as Renilla luciferase. This normalization step can be performed by modern luminometry devices automatically providing final results. Here we demonstrate paclitaxel to strikingly enhance Renilla luminescence, thereby potentially flawing results from reporter gene assays. In consequence, these data advocate for careful examination of raw data and militate against automatic data processing. |
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| Item Description: | Gesehen am 03.02.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1096-0309 |
| DOI: | 10.1016/j.ab.2013.02.026 |