Adaptive immunity and pathogenesis of diabetes: insights provided by the α4-integrin deficient NOD mouse
Background: The spontaneously diabetic “non-obese diabetic” (NOD) mouse is a faithful model of human type-1 diabetes (T1D). Methods: Given the pivotal role of α4 integrin (CD49d) in other autoimmune diseases, we generated NOD mice with α4-deficient hematopoiesis (NOD.α4-/-) to study the role of α4 i...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
4 December 2020
|
| In: |
Cells
Year: 2020, Volume: 9, Issue: 12, Pages: 1-16 |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells9122597 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cells9122597 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2073-4409/9/12/2597 
|
| Author Notes: | Salim Oulghazi, Sarah K. Wegner, Gabriele Spohn, Nina Müller, Sabine Harenkamp, Albrecht Stenzinger, Thalia Papayannopoulou and Halvard Bonig |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1788881311 | ||
| 003 | DE-627 | ||
| 005 | 20250106002932.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220208s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cells9122597 |2 doi | |
| 035 | |a (DE-627)1788881311 | ||
| 035 | |a (DE-599)KXP1788881311 | ||
| 035 | |a (OCoLC)1341441416 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Oulghazi, Salim |e VerfasserIn |0 (DE-588)1245020161 |0 (DE-627)1776174607 |4 aut | |
| 245 | 1 | 0 | |a Adaptive immunity and pathogenesis of diabetes |b insights provided by the α4-integrin deficient NOD mouse |c Salim Oulghazi, Sarah K. Wegner, Gabriele Spohn, Nina Müller, Sabine Harenkamp, Albrecht Stenzinger, Thalia Papayannopoulou and Halvard Bonig |
| 246 | 3 | 0 | |a four |
| 246 | 3 | 3 | |a Adaptive immunity and pathogenesis of diabetes$dinsights provided by the alpha4-integrin deficient NOD mouse |
| 264 | 1 | |c 4 December 2020 | |
| 300 | |a 16 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.02.2022 | ||
| 520 | |a Background: The spontaneously diabetic “non-obese diabetic” (NOD) mouse is a faithful model of human type-1 diabetes (T1D). Methods: Given the pivotal role of α4 integrin (CD49d) in other autoimmune diseases, we generated NOD mice with α4-deficient hematopoiesis (NOD.α4-/-) to study the role of α4 integrin in T1D. Results: NOD.α4-/- mice developed islet-specific T-cells and antibodies, albeit quantitatively less than α4+ counterparts. Nevertheless, NOD.α4-/- mice were completely and life-long protected from diabetes and insulitis. Moreover, transplantation with isogeneic α4-/- bone marrow prevented progression to T1D of pre-diabetic NOD.α4+ mice despite significant pre-existing islet cell injury. Transfer of α4+/CD3+, but not α4+/CD4+ splenocytes from diabetic to NOD.α4-/- mice induced diabetes with short latency. Despite an only modest contribution of adoptively transferred α4+/CD3+ cells to peripheral blood, pancreas-infiltrating T-cells were exclusively graft derived, i.e., α4+. Microbiota of diabetes-resistant NOD.α4-/- and pre-diabetic NOD.α4+ mice were identical. Co- housed diabetic NOD.α4+ mice showed the characteristic diabetic dysbiosis, implying causality of diabetes for dysbiosis. Incidentally, NOD.α4-/- mice were protected from autoimmune sialitis. Conclusion: α4 is a potential target for primary or secondary prevention of T1D. | ||
| 650 | 4 | |a autoimmune diabetes | |
| 650 | 4 | |a CD49d | |
| 650 | 4 | |a integrin | |
| 650 | 4 | |a sialitis | |
| 650 | 4 | |a type-1-diabetes | |
| 650 | 4 | |a VLA4 | |
| 700 | 1 | |a Wegner, Sarah K. |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Spohn, Gabriele |d 1969- |e VerfasserIn |0 (DE-588)139361774 |0 (DE-627)703302094 |0 (DE-576)311375979 |4 aut | |
| 700 | 1 | |a Müller, Nina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Harenkamp, Sabine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Stenzinger, Albrecht |e VerfasserIn |0 (DE-588)139606106 |0 (DE-627)703395238 |0 (DE-576)312432755 |4 aut | |
| 700 | 1 | |a Papayannopoulou, Thalia |e VerfasserIn |4 aut | |
| 700 | 1 | |8 2\p |a Bönig, Halvard |d 1967- |e VerfasserIn |0 (DE-588)120496100 |0 (DE-627)696726009 |0 (DE-576)29224973X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cells |d Basel : MDPI, 2012 |g 9(2020), 12, Artikel-ID 2597, Seite 1-16 |h Online-Ressource |w (DE-627)718622081 |w (DE-600)2661518-6 |w (DE-576)365412112 |x 2073-4409 |7 nnas |a Adaptive immunity and pathogenesis of diabetes insights provided by the α4-integrin deficient NOD mouse |
| 773 | 1 | 8 | |g volume:9 |g year:2020 |g number:12 |g elocationid:2597 |g pages:1-16 |g extent:16 |a Adaptive immunity and pathogenesis of diabetes insights provided by the α4-integrin deficient NOD mouse |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cells9122597 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2073-4409/9/12/2597 |x Verlag |z lizenzpflichtig |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 2\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20220208 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 139606106 |a Stenzinger, Albrecht |m 139606106:Stenzinger, Albrecht |d 910000 |d 912000 |e 910000PS139606106 |e 912000PS139606106 |k 0/910000/ |k 1/910000/912000/ |p 6 | ||
| 998 | |g 1245020161 |a Oulghazi, Salim |m 1245020161:Oulghazi, Salim |d 910000 |d 910100 |e 910000PO1245020161 |e 910100PO1245020161 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1788881311 |e 4053366437 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1788881311","person":[{"given":"Salim","display":"Oulghazi, Salim","family":"Oulghazi","role":"aut"},{"role":"aut","display":"Wegner, Sarah K.","family":"Wegner","given":"Sarah K."},{"family":"Spohn","display":"Spohn, Gabriele","role":"aut","given":"Gabriele"},{"role":"aut","family":"Müller","display":"Müller, Nina","given":"Nina"},{"given":"Sabine","role":"aut","family":"Harenkamp","display":"Harenkamp, Sabine"},{"given":"Albrecht","family":"Stenzinger","display":"Stenzinger, Albrecht","role":"aut"},{"role":"aut","family":"Papayannopoulou","display":"Papayannopoulou, Thalia","given":"Thalia"},{"given":"Halvard","role":"aut","family":"Bönig","display":"Bönig, Halvard"}],"titleAlt":[{"title":"Adaptive immunity and pathogenesis of diabetes$dinsights provided by the alpha4-integrin deficient NOD mouse"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 08.02.2022"],"name":{"displayForm":["Salim Oulghazi, Sarah K. Wegner, Gabriele Spohn, Nina Müller, Sabine Harenkamp, Albrecht Stenzinger, Thalia Papayannopoulou and Halvard Bonig"]},"physDesc":[{"extent":"16 S."}],"title":[{"title":"Adaptive immunity and pathogenesis of diabetes","subtitle":"insights provided by the α4-integrin deficient NOD mouse","title_sort":"Adaptive immunity and pathogenesis of diabetes"}],"language":["eng"],"origin":[{"dateIssuedDisp":"4 December 2020","dateIssuedKey":"2020"}],"id":{"eki":["1788881311"],"doi":["10.3390/cells9122597"]},"relHost":[{"note":["Gesehen am 09.05.12"],"title":[{"title_sort":"Cells","title":"Cells","subtitle":"open access journal"}],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"origin":[{"dateIssuedDisp":"2012-","dateIssuedKey":"2012","publisherPlace":"Basel","publisher":"MDPI"}],"id":{"issn":["2073-4409"],"zdb":["2661518-6"],"eki":["718622081"]},"part":{"text":"9(2020), 12, Artikel-ID 2597, Seite 1-16","extent":"16","year":"2020","issue":"12","volume":"9","pages":"1-16"},"disp":"Adaptive immunity and pathogenesis of diabetes insights provided by the α4-integrin deficient NOD mouseCells","pubHistory":["1.2012 -"],"type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"718622081"}]} | ||
| SRT | |a OULGHAZISAADAPTIVEIM4202 | ||