The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells
Microorganisms use multiple two-component sensory systems to detect changes in their environment and elicit physiological responses. Despite their wide spread and importance, the intracellular organization of two-component sensory proteins in bacteria remains little investigated. A notable exception...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
|
| In: |
PLOS ONE
Year: 2013, Jahrgang: 8, Heft: 10, Pages: 1-11 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0077708 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1371/journal.pone.0077708 Verlag, kostenfrei, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077708 
|
| Verfasserangaben: | Erik Sommer, Moriah Koler, Vered Frank, Victor Sourjik, Ady Vaknin |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1789244390 | ||
| 003 | DE-627 | ||
| 005 | 20220820125337.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220210s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0077708 |2 doi | |
| 035 | |a (DE-627)1789244390 | ||
| 035 | |a (DE-599)KXP1789244390 | ||
| 035 | |a (OCoLC)1341441458 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Sommer, Erik |e VerfasserIn |0 (DE-588)1021169269 |0 (DE-627)691488592 |0 (DE-576)362664803 |4 aut | |
| 245 | 1 | 4 | |a The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells |c Erik Sommer, Moriah Koler, Vered Frank, Victor Sourjik, Ady Vaknin |
| 264 | 1 | |c 2013 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published: October 11, 2013 | ||
| 500 | |a Gesehen am 10.02.2022 | ||
| 520 | |a Microorganisms use multiple two-component sensory systems to detect changes in their environment and elicit physiological responses. Despite their wide spread and importance, the intracellular organization of two-component sensory proteins in bacteria remains little investigated. A notable exception is the well-studied clustering of the chemoreceptor-kinase complexes that mediate chemotaxis behaviour. However, these chemosensory complexes differ fundamentally from other systems, both structurally and functionally. Therefore, studying the organization of typical sensory kinases in bacteria is essential for understanding the general role of receptor clustering in bacterial sensory signalling. Here, by studying mYFP-tagged sensory kinases in Escherichia coli, we show that the tagged TorS and EvgS sensors have a clear tendency for self-association and clustering. These sensors clustered even when expressed at a level of a few hundred copies per cell. Moreover, the mYFP-tagged response regulator TorR showed clear TorS-dependent clustering, indicating that untagged TorS sensors also tend to form clusters. We also provide evidence for the functionality of these tagged sensors. Experiments with truncated TorS or EvgS proteins suggested that clustering of EvgS sensors depends on the cytoplasmic part of the protein, whereas clustering of TorS sensors can be potentially mediated by the periplasmic/transmembrane domain. Overall, these findings support the notion that sensor clustering plays a role in bacterial sensory signalling beyond chemotaxis. | ||
| 650 | 4 | |a Arabinose | |
| 650 | 4 | |a Cell fusion | |
| 650 | 4 | |a Chemotaxis | |
| 650 | 4 | |a Fluorescence | |
| 650 | 4 | |a Fluorescence imaging | |
| 650 | 4 | |a Fluorescence resonance energy transfer | |
| 650 | 4 | |a Protein domains | |
| 650 | 4 | |a Sensory perception | |
| 700 | 1 | |a Koler, Moriah |e VerfasserIn |4 aut | |
| 700 | 1 | |a Frank, Vered |e VerfasserIn |4 aut | |
| 700 | 1 | |a Sourjik, Victor |d 1970- |e VerfasserIn |0 (DE-588)1020549998 |0 (DE-627)69125737X |0 (DE-576)360687725 |4 aut | |
| 700 | 1 | |a Vaknin, Ady |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 8(2013), 10 vom: Okt., Artikel-ID e77708, Seite 1-11 |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells |
| 773 | 1 | 8 | |g volume:8 |g year:2013 |g number:10 |g month:10 |g elocationid:e77708 |g pages:1-11 |g extent:11 |a The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pone.0077708 |x Verlag |x Resolving-System |z kostenfrei |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077708 |x Verlag |z kostenfrei |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220210 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 1020549998 |a Sourjik, Victor |m 1020549998:Sourjik, Victor |d 700000 |d 706000 |e 700000PS1020549998 |e 706000PS1020549998 |k 0/700000/ |k 1/700000/706000/ |p 4 | ||
| 998 | |g 1021169269 |a Sommer, Erik |m 1021169269:Sommer, Erik |d 700000 |d 706000 |e 700000PS1021169269 |e 706000PS1021169269 |k 0/700000/ |k 1/700000/706000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1789244390 |e 4055670060 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Published: October 11, 2013","Gesehen am 10.02.2022"],"language":["eng"],"recId":"1789244390","title":[{"title":"The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells","title_sort":"sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli Cells"}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Sommer, Erik","given":"Erik","family":"Sommer"},{"given":"Moriah","family":"Koler","role":"aut","display":"Koler, Moriah","roleDisplay":"VerfasserIn"},{"family":"Frank","given":"Vered","display":"Frank, Vered","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Sourjik","given":"Victor","roleDisplay":"VerfasserIn","display":"Sourjik, Victor","role":"aut"},{"given":"Ady","family":"Vaknin","role":"aut","display":"Vaknin, Ady","roleDisplay":"VerfasserIn"}],"physDesc":[{"extent":"11 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Public Library of Science"]},"origin":[{"dateIssuedDisp":"2006-","publisher":"PLOS ; PLoS","dateIssuedKey":"2006","publisherPlace":"San Francisco, California, US ; Lawrence, Kan."}],"id":{"issn":["1932-6203"],"zdb":["2267670-3"],"eki":["523574592"]},"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"disp":"The sensory histidine kinases TorS and EvgS tend to form clusters in Escherichia coli CellsPLOS ONE","type":{"bibl":"periodical","media":"Online-Ressource"},"recId":"523574592","language":["eng"],"corporate":[{"display":"Public Library of Science","roleDisplay":"Herausgebendes Organ","role":"isb"}],"pubHistory":["1.2006 -"],"part":{"issue":"10","pages":"1-11","year":"2013","extent":"11","volume":"8","text":"8(2013), 10 vom: Okt., Artikel-ID e77708, Seite 1-11"},"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}]}],"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"2013"}],"id":{"doi":["10.1371/journal.pone.0077708"],"eki":["1789244390"]},"name":{"displayForm":["Erik Sommer, Moriah Koler, Vered Frank, Victor Sourjik, Ady Vaknin"]}} | ||
| SRT | |a SOMMERERIKSENSORYHIS2013 | ||