Transient transfection of plasmodium vivax blood-stage parasites

Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating inte...

Full description

Saved in:
Bibliographic Details
Main Authors: Sanchez, Cecilia P. (Author) , Pfahler, Judith Martha (Author) , del Portillo, Hernando A. (Author) , Lanzer, Michael (Author)
Format: Chapter/Article
Language:English
Published: 2013
In: Malaria
Year: 2013, Pages: 151-159
DOI:10.1007/978-1-62703-026-7_10
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-62703-026-7_10
Get full text
Author Notes:Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer

MARC

LEADER 00000caa a22000002c 4500
001 1789934877
003 DE-627
005 20230426075826.0
007 cr uuu---uuuuu
008 220217s2013 xx |||||o 00| ||eng c
020 |a 9781627030267  |9 978-1-62703-026-7 
024 7 |a 10.1007/978-1-62703-026-7_10  |2 doi 
035 |a (DE-627)1789934877 
035 |a (DE-599)KXP1789934877 
035 |a (OCoLC)1341441860 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Sanchez, Cecilia P.  |e VerfasserIn  |0 (DE-588)1079241388  |0 (DE-627)840669348  |0 (DE-576)452286093  |4 aut 
245 1 0 |a Transient transfection of plasmodium vivax blood-stage parasites  |c Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer 
264 1 |c 2013 
300 |a 9 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a First online: 25 August 2012 
500 |a Gesehen am 17.02.2022 
520 |a Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating interactions with the human host still awaits further technological breakthroughs, foremost the establishment of a continuous in vitro culture system. Nevertheless, the first steps towards domesticating P. vivax for research purposes have been made. Transfection will eventually help to better understand the unique pathogenic features displayed by P. vivax, such as the host cell specificity for reticulocytes and the occurrence of relapses. Transfection will also be an invaluable tool for studies related to the emerging drug resistance, and it will help identify and validate novel targets for rational intervention. 
650 4 |a Ex vivo culture 
650 4 |a Luciferase assay 
650 4 |a P. vivax 
650 4 |a Saimiri monkey 
650 4 |a Transfection 
700 1 |a Pfahler, Judith Martha  |e VerfasserIn  |0 (DE-588)13240494X  |0 (DE-627)522675808  |0 (DE-576)299124940  |4 aut 
700 1 |a del Portillo, Hernando A.  |e VerfasserIn  |4 aut 
700 1 |a Lanzer, Michael  |e VerfasserIn  |0 (DE-588)1065643314  |0 (DE-627)816506736  |0 (DE-576)165563826  |4 aut 
773 0 8 |i Enthalten in  |t Malaria  |b 2nd ed. 2013  |d Totowa, NJ : Humana Press, 2013  |g (2013), Seite 151-159  |h Online-Ressource (XV, 626 p. 75 illus., 53 illus. in color, digital)  |w (DE-627)1651868395  |w (DE-576)373500548  |z 9781627030267  |7 nnam  |a Transient transfection of plasmodium vivax blood-stage parasites 
773 1 8 |g year:2013  |g pages:151-159  |g extent:9  |a Transient transfection of plasmodium vivax blood-stage parasites 
856 4 0 |u https://doi.org/10.1007/978-1-62703-026-7_10  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20220217 
993 |a BookComponentPart 
994 |a 2013 
998 |g 1065643314  |a Lanzer, Michael  |m 1065643314:Lanzer, Michael  |d 910000  |d 911700  |e 910000PL1065643314  |e 911700PL1065643314  |k 0/910000/  |k 1/910000/911700/  |p 4  |y j 
998 |g 1079241388  |a Sanchez, Cecilia P.  |m 1079241388:Sanchez, Cecilia P.  |d 910000  |d 911700  |e 910000PS1079241388  |e 911700PS1079241388  |k 0/910000/  |k 1/910000/911700/  |p 1  |x j 
999 |a KXP-PPN1789934877  |e 4060998680 
BIB |a Y 
JSO |a {"type":{"media":"Online-Ressource","bibl":"chapter"},"note":["First online: 25 August 2012","Gesehen am 17.02.2022"],"language":["eng"],"recId":"1789934877","person":[{"family":"Sanchez","given":"Cecilia P.","roleDisplay":"VerfasserIn","display":"Sanchez, Cecilia P.","role":"aut"},{"family":"Pfahler","given":"Judith Martha","roleDisplay":"VerfasserIn","display":"Pfahler, Judith Martha","role":"aut"},{"given":"Hernando A.","family":"del Portillo","role":"aut","display":"del Portillo, Hernando A.","roleDisplay":"VerfasserIn"},{"given":"Michael","family":"Lanzer","role":"aut","roleDisplay":"VerfasserIn","display":"Lanzer, Michael"}],"title":[{"title":"Transient transfection of plasmodium vivax blood-stage parasites","title_sort":"Transient transfection of plasmodium vivax blood-stage parasites"}],"physDesc":[{"extent":"9 S."}],"relHost":[{"relMultPart":[{"dispAlt":"Methods in molecular biology","physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedKey":"1984","publisher":"Humana Press ; [Springer]","dateIssuedDisp":"1984-","publisherPlace":"Totowa, NJ ; [Heidelberg]"}],"id":{"issn":["1940-6029"],"zdb":["2493551-7"],"eki":["599671882"]},"pubHistory":["1.1984 -"],"part":{"number_sort":["923"],"number":["923"]},"type":{"bibl":"serial","media":"Online-Ressource"},"disp":"Methods in Molecular Biology","note":["Gesehen am 05.11.12"],"language":["eng"],"recId":"599671882","title":[{"title_sort":"Methods in molecular biology","title":"Methods in molecular biology"}]}],"physDesc":[{"extent":"Online-Ressource (XV, 626 p. 75 illus., 53 illus. in color, digital)"}],"origin":[{"dateIssuedDisp":"2013","publisher":"Humana Press","edition":"2nd ed. 2013","publisherPlace":"Totowa, NJ","editionNo":2,"dateIssuedKey":"2013"}],"id":{"doi":["10.1007/978-1-62703-026-7"],"eki":["1651868395"],"isbn":["9781627030267"]},"name":{"displayForm":["edited by Robert Ménard"]},"part":{"extent":"9","text":"(2013), Seite 151-159","pages":"151-159","year":"2013"},"type":{"media":"Online-Ressource","bibl":"edited-book"},"disp":"Transient transfection of plasmodium vivax blood-stage parasitesMalaria","recId":"1651868395","language":["eng"],"title":[{"title_sort":"Malaria","subtitle":"methods and protocols","title":"Malaria"}],"person":[{"role":"edt","roleDisplay":"Hrsg.","display":"Ménard, Robert","given":"Robert","family":"Ménard"}]}],"name":{"displayForm":["Cecilia P. Sanchez, Judith Pfahler, Hernando A. del Portillo, Michael Lanzer"]},"origin":[{"dateIssuedDisp":"2013","dateIssuedKey":"2013"}],"id":{"isbn":["9781627030267"],"eki":["1789934877"],"doi":["10.1007/978-1-62703-026-7_10"]}} 
SRT |a SANCHEZCECTRANSIENTT2013