Lentiviral vector integration profiles differ in rodent postmitotic tissues
Lentiviral vectors with self-inactivating (SIN) long terminal repeats (LTRs) are promising for safe and sustained transgene expression in dividing as well as quiescent cells. As genome organization and transcription substantially differs between actively dividing and postmitotic cells in vivo, we hy...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
|
| In: |
Molecular therapy
Year: 2011, Volume: 19, Issue: 4, Pages: 703-710 |
| ISSN: | 1525-0024 |
| DOI: | 10.1038/mt.2011.19 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/mt.2011.19 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1525001616304269 
|
| Author Notes: | Cynthia C Bartholomae, Anne Arens, Kamaljit S Balaggan, Rafael J Yáñez-Muñoz, Eugenio Montini, Steven J Howe, Anna Paruzynski, Bernhard Korn, Jens Uwe Appelt, Angus MacNeil, Daniela Cesana, Ulrich Abel, Hanno Glimm, Luigi Naldini, Robin R Ali, Adrian J Thrasher, Christof von Kalle and Manfred Schmidt |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1789975670 | ||
| 003 | DE-627 | ||
| 005 | 20230426075831.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220217s2011 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/mt.2011.19 |2 doi | |
| 035 | |a (DE-627)1789975670 | ||
| 035 | |a (DE-599)KXP1789975670 | ||
| 035 | |a (OCoLC)1341441751 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Bartholomä, Cynthia C. |e VerfasserIn |0 (DE-588)1036481042 |0 (DE-627)751079243 |0 (DE-576)384489524 |4 aut | |
| 245 | 1 | 0 | |a Lentiviral vector integration profiles differ in rodent postmitotic tissues |c Cynthia C Bartholomae, Anne Arens, Kamaljit S Balaggan, Rafael J Yáñez-Muñoz, Eugenio Montini, Steven J Howe, Anna Paruzynski, Bernhard Korn, Jens Uwe Appelt, Angus MacNeil, Daniela Cesana, Ulrich Abel, Hanno Glimm, Luigi Naldini, Robin R Ali, Adrian J Thrasher, Christof von Kalle and Manfred Schmidt |
| 264 | 1 | |c 2011 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a First published online: 14 December 2016 | ||
| 500 | |a Gesehen am 17.02.2022 | ||
| 520 | |a Lentiviral vectors with self-inactivating (SIN) long terminal repeats (LTRs) are promising for safe and sustained transgene expression in dividing as well as quiescent cells. As genome organization and transcription substantially differs between actively dividing and postmitotic cells in vivo, we hypothesized that genomic vector integration preferences might be distinct between these biological states. We performed integration site (IS) analyses on mouse dividing cells (fibroblasts and hematopoietic progenitor cells (HPCs)) transduced ex vivo and postmitotic cells (eye and brain) transduced in vivo. As expected, integration in dividing cells occurred preferably into gene coding regions. In contrast, postmitotic cells showed a close to random frequency of integration into genes and gene spare long interspersed nuclear elements (LINE). Our studies on the potential mechanisms responsible for the detected differences of lentiviral integration suggest that the lowered expression level of Psip1 reduce the integration frequency in vivo into gene coding regions in postmitotic cells. The motif TGGAA might represent one of the factors for preferred lentiviral integration into mouse and rat Satellite DNA. These observations are highly relevant for the correct assessment of preclinical biosafety studies, indicating that lentiviral vectors are well suited for safe and effective clinical gene transfer into postmitotic tissues. | ||
| 700 | 1 | |a Arens, Anne |d 1980- |e VerfasserIn |0 (DE-588)1030235244 |0 (DE-627)734950039 |0 (DE-576)378039490 |4 aut | |
| 700 | 1 | |a Balaggan, Kamaljit S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Yáñez-Muñoz, Rafael J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Montini, Eugenio |e VerfasserIn |4 aut | |
| 700 | 1 | |a Howe, Steven J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Paruzynski, Anna |e VerfasserIn |0 (DE-588)142778648 |0 (DE-627)640120822 |0 (DE-576)333342526 |4 aut | |
| 700 | 1 | |a Korn, Bernhard |e VerfasserIn |0 (DE-588)1163822922 |0 (DE-627)1028164270 |0 (DE-576)508230853 |4 aut | |
| 700 | 1 | |a Appelt, Jens-Uwe |d 1978- |e VerfasserIn |0 (DE-588)1013120035 |0 (DE-627)663353564 |0 (DE-576)346002117 |4 aut | |
| 700 | 1 | |a MacNeil, Angus |e VerfasserIn |4 aut | |
| 700 | 1 | |a Cesana, Daniela |e VerfasserIn |4 aut | |
| 700 | 1 | |a Abel, Ulrich |d 1952- |e VerfasserIn |0 (DE-588)14287552X |0 (DE-627)640555403 |0 (DE-576)333774582 |4 aut | |
| 700 | 1 | |a Glimm, Hanno |d 1967- |e VerfasserIn |0 (DE-588)113880677 |0 (DE-627)51985120X |0 (DE-576)289777879 |4 aut | |
| 700 | 1 | |a Naldini, Luigi |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ali, Robin R. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Thrasher, Adrian J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kalle, Christof von |d 1962- |e VerfasserIn |0 (DE-588)1036481115 |0 (DE-627)75107926X |0 (DE-576)168957396 |4 aut | |
| 700 | 1 | |a Schmidt, Manfred |d 1967-2022 |e VerfasserIn |0 (DE-588)122922301 |0 (DE-627)706092430 |0 (DE-576)293477620 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular therapy |d Amsterdam : Elsevier, 2000 |g 19(2011), 4 vom: Apr., Seite 703-710 |h Online-Ressource |w (DE-627)320415198 |w (DE-600)2001818-6 |w (DE-576)104344695 |x 1525-0024 |7 nnas |a Lentiviral vector integration profiles differ in rodent postmitotic tissues |
| 773 | 1 | 8 | |g volume:19 |g year:2011 |g number:4 |g month:04 |g pages:703-710 |g extent:8 |a Lentiviral vector integration profiles differ in rodent postmitotic tissues |
| 776 | 0 | 8 | |i Erscheint auch als |n Druck-Ausgabe |a Bartholomä, Cynthia C. |t Lentiviral vector integration profiles differ in rodent postmitotic tissues |d 2011 |w (DE-627)1632289008 |w (DE-576)384506208 |
| 856 | 4 | 0 | |u https://doi.org/10.1038/mt.2011.19 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S1525001616304269 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220217 | ||
| 993 | |a Article | ||
| 994 | |a 2011 | ||
| 998 | |g 122922301 |a Schmidt, Manfred |m 122922301:Schmidt, Manfred |d 140000 |e 140000PS122922301 |k 0/140000/ |p 18 |y j | ||
| 998 | |g 1036481115 |a Kalle, Christof von |m 1036481115:Kalle, Christof von |d 910000 |e 910000PK1036481115 |k 0/910000/ |p 17 | ||
| 998 | |g 113880677 |a Glimm, Hanno |m 113880677:Glimm, Hanno |d 910000 |e 910000PG113880677 |k 0/910000/ |p 13 | ||
| 998 | |g 14287552X |a Abel, Ulrich |m 14287552X:Abel, Ulrich |d 910000 |d 999701 |d 50000 |e 910000PA14287552X |e 999701PA14287552X |e 50000PA14287552X |k 0/910000/ |k 1/910000/999701/ |k 0/50000/ |p 12 | ||
| 998 | |g 1013120035 |a Appelt, Jens-Uwe |m 1013120035:Appelt, Jens-Uwe |d 50000 |e 50000PA1013120035 |k 0/50000/ |p 9 | ||
| 998 | |g 1163822922 |a Korn, Bernhard |m 1163822922:Korn, Bernhard |d 50000 |e 50000PK1163822922 |k 0/50000/ |p 8 | ||
| 998 | |g 142778648 |a Paruzynski, Anna |m 142778648:Paruzynski, Anna |d 140000 |e 140000PP142778648 |k 0/140000/ |p 7 | ||
| 998 | |g 1030235244 |a Arens, Anne |m 1030235244:Arens, Anne |p 2 | ||
| 998 | |g 1036481042 |a Bartholomä, Cynthia C. |m 1036481042:Bartholomä, Cynthia C. |d 50000 |e 50000PB1036481042 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1789975670 |e 4061104748 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedDisp":"2011","dateIssuedKey":"2011"}],"id":{"eki":["1789975670"],"doi":["10.1038/mt.2011.19"]},"recId":"1789975670","name":{"displayForm":["Cynthia C Bartholomae, Anne Arens, Kamaljit S Balaggan, Rafael J Yáñez-Muñoz, Eugenio Montini, Steven J Howe, Anna Paruzynski, Bernhard Korn, Jens Uwe Appelt, Angus MacNeil, Daniela Cesana, Ulrich Abel, Hanno Glimm, Luigi Naldini, Robin R Ali, Adrian J Thrasher, Christof von Kalle and Manfred Schmidt"]},"title":[{"title":"Lentiviral vector integration profiles differ in rodent postmitotic tissues","title_sort":"Lentiviral vector integration profiles differ in rodent postmitotic tissues"}],"person":[{"family":"Bartholomä","role":"aut","given":"Cynthia C.","display":"Bartholomä, Cynthia C."},{"display":"Arens, Anne","family":"Arens","given":"Anne","role":"aut"},{"family":"Balaggan","given":"Kamaljit S.","role":"aut","display":"Balaggan, Kamaljit S."},{"display":"Yáñez-Muñoz, Rafael J.","role":"aut","given":"Rafael J.","family":"Yáñez-Muñoz"},{"given":"Eugenio","role":"aut","family":"Montini","display":"Montini, Eugenio"},{"display":"Howe, Steven J.","role":"aut","given":"Steven J.","family":"Howe"},{"family":"Paruzynski","role":"aut","given":"Anna","display":"Paruzynski, Anna"},{"given":"Bernhard","role":"aut","family":"Korn","display":"Korn, Bernhard"},{"display":"Appelt, Jens-Uwe","family":"Appelt","given":"Jens-Uwe","role":"aut"},{"role":"aut","given":"Angus","family":"MacNeil","display":"MacNeil, Angus"},{"display":"Cesana, Daniela","given":"Daniela","role":"aut","family":"Cesana"},{"display":"Abel, Ulrich","family":"Abel","role":"aut","given":"Ulrich"},{"display":"Glimm, Hanno","family":"Glimm","given":"Hanno","role":"aut"},{"family":"Naldini","given":"Luigi","role":"aut","display":"Naldini, Luigi"},{"display":"Ali, Robin R.","given":"Robin R.","role":"aut","family":"Ali"},{"display":"Thrasher, Adrian J.","family":"Thrasher","role":"aut","given":"Adrian J."},{"given":"Christof von","role":"aut","family":"Kalle","display":"Kalle, Christof von"},{"family":"Schmidt","given":"Manfred","role":"aut","display":"Schmidt, Manfred"}],"physDesc":[{"extent":"8 S."}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"relHost":[{"part":{"text":"19(2011), 4 vom: Apr., Seite 703-710","extent":"8","volume":"19","pages":"703-710","year":"2011","issue":"4"},"id":{"eki":["320415198"],"zdb":["2001818-6"],"issn":["1525-0024"]},"origin":[{"publisher":"Elsevier ; Nature Publ. Group ; Acad. Press","dateIssuedKey":"2000","dateIssuedDisp":"2000-","publisherPlace":"Amsterdam ; New York, NY ; Orlando, Fla."}],"disp":"Lentiviral vector integration profiles differ in rodent postmitotic tissuesMolecular therapy","recId":"320415198","title":[{"subtitle":"official journal of the American Society of Gene Therapy","title":"Molecular therapy","title_sort":"Molecular therapy"}],"pubHistory":["1.2000 -"],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"corporate":[{"role":"isb","display":"American Society of Gene Therapy"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 28.05.2020"]}],"note":["First published online: 14 December 2016","Gesehen am 17.02.2022"]} | ||
| SRT | |a BARTHOLOMALENTIVIRAL2011 | ||