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Single-cell PCR analysis of the immunoglobulin heavy-chain CDR3 region for the diagnosis of leptomeningeal involvement of B-cell malignancies using standard cerebrospinal fluid cytospins

The diagnosis of leptomeningeal B-cell malignancies is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). We have established a polymerase chain reaction (PCR) approach to characterize the clonally diverse gene encoding the immunoglobulin heavy-chain (IgH) third compl...

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Main Authors: Hug, Andreas (Author) , Storch-Hagenlocher, Brigitte (Author) , Haas, Jürgen (Author) , Vogt-Schaden, Maria-Elisabeth (Author) , Goldschmidt, Hartmut (Author) , Wildemann, Brigitte (Author)
Format: Article (Journal)
Language:English
Published: [2004]
In: Journal of the neurological sciences
Year: 2004, Volume: 219, Issue: 1, Pages: 83-88
ISSN:1878-5883
DOI:10.1016/j.jns.2003.12.012
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jns.2003.12.012
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022510X03003873
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Author Notes:Andreas Hug, Brigitte Storch-Hagenlocher, Juergen Haas, Maria-Elisabeth Vogt-Schaden, Hartmut Goldschmidt, Brigitte Wildemann
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Summary:The diagnosis of leptomeningeal B-cell malignancies is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). We have established a polymerase chain reaction (PCR) approach to characterize the clonally diverse gene encoding the immunoglobulin heavy-chain (IgH) third complementarity determining region (CDR3) of single B cells. We demonstrate that single-cell PCR is readily applicable to individual cells derived from routine CSF cytospins and is a powerful method to discriminate monoclonal neoplastic from polyclonal reactive B-cell responses. Single-cell PCR analysis, as a new tool for the diagnosis and monitoring of neoplastic meningitis associated with B-cell malignancies, is particularly important if cytology, immunocytochemistry, flow cytometry and automated gene scanning of CSF samples are unable to detect malignant monoclonal proliferation.
Item Description:Gesehen am 25.02.2022
Physical Description:Online Resource
ISSN:1878-5883
DOI:10.1016/j.jns.2003.12.012

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