Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation
The clinical relevance of the assessment of minimal residual disease (MRD) in patients with multiple myeloma (MM) to predict disease recurrence has not been proven. In the present study, we retrospectively analyzed the tumor load in peripheral blood (PB) and bone marrow (BM) samples of 13 patients w...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
29 November 2001
|
| In: |
Bone marrow transplantation
Year: 2001, Jahrgang: 28, Heft: 10, Pages: 957-962 |
| ISSN: | 1476-5365 |
| DOI: | 10.1038/sj.bmt.1703276 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/sj.bmt.1703276 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/1703276 
|
| Verfasserangaben: | E. Lipinski, F. W. Cremer, A. D. Ho, H. Goldschmidt, M. Moos (Medizinische Klinik und Poliklinik V, Universität Heidelberg, Heidelberg, Germany) |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1794154760 | ||
| 003 | DE-627 | ||
| 005 | 20230426180458.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220301s2001 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/sj.bmt.1703276 |2 doi | |
| 035 | |a (DE-627)1794154760 | ||
| 035 | |a (DE-599)KXP1794154760 | ||
| 035 | |a (OCoLC)1341445607 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Lipinski, Emanuel |e VerfasserIn |0 (DE-588)1252699972 |0 (DE-627)1794156119 |4 aut | |
| 245 | 1 | 0 | |a Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation |c E. Lipinski, F. W. Cremer, A. D. Ho, H. Goldschmidt, M. Moos (Medizinische Klinik und Poliklinik V, Universität Heidelberg, Heidelberg, Germany) |
| 264 | 1 | |c 29 November 2001 | |
| 300 | |b Diagramme | ||
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 01.03.2022 | ||
| 520 | |a The clinical relevance of the assessment of minimal residual disease (MRD) in patients with multiple myeloma (MM) to predict disease recurrence has not been proven. In the present study, we retrospectively analyzed the tumor load in peripheral blood (PB) and bone marrow (BM) samples of 13 patients with MM both in remission after high-dose therapy (HDT) with autologous PBSC transplantation (PBSCT) and at the time of progressive disease (PD). For six patients, subsequent samples obtained in remission could be included in the study. Tumor cells were assessed by means of quantitative PCR with allele-specific oligonucleotides (ASO-qPCR) based on the method of limiting dilutions. PD was documented with ASO-qPCR in BM samples (median concentration of tumor cells in remission vs at PD: 0.18% vs 4.6%) representing a significant increase by a median factor of 8.7. In PB, the median tumor load was 799 cells/ml in remission and 23 400 cells/ml at PD. With a median factor of 45, the increase was much more pronounced. Comparing the results of the molecular monitoring in PB with those of the determination of the monoclonal protein, routinely applied as parameter for the course of the disease, revealed a superiority of the molecular monitoring because of the significantly higher increase in the tumor load. Analyzing the subsequent remission samples showed an increase of the malignant cells in four out of six PB samples and in all four BM samples available, indicating the potential of ASO-qPCR for an early PD recognition. Bone Marrow Transplantation (2001) 28, 957-962. | ||
| 650 | 4 | |a Cell Biology | |
| 650 | 4 | |a general | |
| 650 | 4 | |a Hematology | |
| 650 | 4 | |a Internal Medicine | |
| 650 | 4 | |a Medicine/Public Health | |
| 650 | 4 | |a Public Health | |
| 650 | 4 | |a Stem Cells | |
| 700 | 1 | |a Cremer, Friedrich Walter |d 1967- |e VerfasserIn |0 (DE-588)115550461 |0 (DE-627)691396221 |0 (DE-576)289943752 |4 aut | |
| 700 | 1 | |a Ho, Anthony Dick |d 1948- |e VerfasserIn |0 (DE-588)108692477 |0 (DE-627)504904205 |0 (DE-576)19010466X |4 aut | |
| 700 | 1 | |a Goldschmidt, Hartmut |d 1956- |e VerfasserIn |0 (DE-588)102258023X |0 (DE-627)717003809 |0 (DE-576)365637386 |4 aut | |
| 700 | 1 | |a Moos, Marion |e VerfasserIn |0 (DE-588)1243124318 |0 (DE-627)1773573187 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Bone marrow transplantation |d London : Springer Nature, 1997 |g 28(2001), 10 vom: Nov., Seite 957-962 |h Online-Ressource |w (DE-627)320433366 |w (DE-600)2004030-1 |w (DE-576)091012996 |x 1476-5365 |7 nnas |a Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation |
| 773 | 1 | 8 | |g volume:28 |g year:2001 |g number:10 |g month:11 |g pages:957-962 |g extent:6 |a Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation |
| 856 | 4 | 0 | |u https://doi.org/10.1038/sj.bmt.1703276 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/1703276 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220301 | ||
| 993 | |a Article | ||
| 994 | |a 2001 | ||
| 998 | |g 1243124318 |a Moos, Marion |m 1243124318:Moos, Marion |d 910000 |d 910100 |e 910000PM1243124318 |e 910100PM1243124318 |k 0/910000/ |k 1/910000/910100/ |p 5 |y j | ||
| 998 | |g 102258023X |a Goldschmidt, Hartmut |m 102258023X:Goldschmidt, Hartmut |d 910000 |d 910100 |e 910000PG102258023X |e 910100PG102258023X |k 0/910000/ |k 1/910000/910100/ |p 4 | ||
| 998 | |g 108692477 |a Ho, Anthony Dick |m 108692477:Ho, Anthony Dick |d 910000 |d 910100 |e 910000PH108692477 |e 910100PH108692477 |k 0/910000/ |k 1/910000/910100/ |p 3 | ||
| 998 | |g 115550461 |a Cremer, Friedrich Walter |m 115550461:Cremer, Friedrich Walter |d 910000 |d 910100 |e 910000PC115550461 |e 910100PC115550461 |k 0/910000/ |k 1/910000/910100/ |p 2 | ||
| 998 | |g 1252699972 |a Lipinski, Emanuel |m 1252699972:Lipinski, Emanuel |d 910000 |d 910100 |e 910000PL1252699972 |e 910100PL1252699972 |k 0/910000/ |k 1/910000/910100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1794154760 |e 4076426452 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation","title":"Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantation"}],"language":["eng"],"origin":[{"dateIssuedKey":"2001","dateIssuedDisp":"29 November 2001"}],"name":{"displayForm":["E. Lipinski, F. W. Cremer, A. D. Ho, H. Goldschmidt, M. Moos (Medizinische Klinik und Poliklinik V, Universität Heidelberg, Heidelberg, Germany)"]},"recId":"1794154760","person":[{"role":"aut","given":"Emanuel","display":"Lipinski, Emanuel","family":"Lipinski"},{"role":"aut","family":"Cremer","given":"Friedrich Walter","display":"Cremer, Friedrich Walter"},{"role":"aut","family":"Ho","given":"Anthony Dick","display":"Ho, Anthony Dick"},{"role":"aut","display":"Goldschmidt, Hartmut","given":"Hartmut","family":"Goldschmidt"},{"family":"Moos","display":"Moos, Marion","given":"Marion","role":"aut"}],"note":["Gesehen am 01.03.2022"],"relHost":[{"title":[{"title":"Bone marrow transplantation","title_sort":"Bone marrow transplantation"}],"origin":[{"publisher":"Springer Nature ; Macmillan ; Nature Publishing Group","dateIssuedDisp":"1997-","dateIssuedKey":"1997","publisherPlace":"London ; Basingstoke ; London"}],"note":["Gesehen am 17.01.25"],"physDesc":[{"extent":"Online-Ressource"}],"part":{"extent":"6","issue":"10","text":"28(2001), 10 vom: Nov., Seite 957-962","volume":"28","year":"2001","pages":"957-962"},"id":{"eki":["320433366"],"issn":["1476-5365"],"zdb":["2004030-1"]},"language":["eng"],"disp":"Molecular monitoring of the tumor load predicts progressive disease in patients with multiple myeloma after high-dose therapy with autologous peripheral blood stem cell transplantationBone marrow transplantation","recId":"320433366","type":{"bibl":"periodical","media":"Online-Ressource"},"pubHistory":["Nachgewiesen 19.1997 -"]}],"id":{"eki":["1794154760"],"doi":["10.1038/sj.bmt.1703276"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"6 S.","noteIll":"Diagramme"}]} | ||
| SRT | |a LIPINSKIEMMOLECULARM2920 | ||