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Transient micro-elastography: a novel non-invasive approach to measure liver stiffness in mice

METHODS: A novel transient micro-elastography (TME) device, dedicated to LS measurements in mice with a range of measurement from 1-170 kPa, was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston. The novel probe was validated in a classical fibrosis model (CCl4) and in a t...

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Main Authors: Bastard, Cècile (Author) , Bosisio, Matteo R. (Author) , Chabert, Michèle (Author) , Kalopissis, Athina D. (Author) , Mahrouf-Yorgov, Meriem (Author) , Gilgenkrantz, Hélène (Author) , Mueller, Sebastian (Author) , Sandrin, Laurent (Author)
Format: Article (Journal)
Language:English
Published: Feb 28, 2011
In: World journal of gastroenterology
Year: 2011, Volume: 17, Issue: 8, Pages: 968-975
ISSN:2219-2840
DOI:10.3748/wjg.v17.i8.968
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3748/wjg.v17.i8.968
Verlag, lizenzpflichtig, Volltext: http://www.wjgnet.com/1007-9327/full/v17/i8/968.htm
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Author Notes:Cécile Bastard, Matteo R Bosisio, Michèle Chabert, Athina D Kalopissis, Meriem Mahrouf-Yorgov, Hélène Gilgenkrantz, Sebastian Mueller, Laurent Sandrin
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Summary:METHODS: A novel transient micro-elastography (TME) device, dedicated to LS measurements in mice with a range of measurement from 1-170 kPa, was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston. The novel probe was validated in a classical fibrosis model (CCl4) and in a transgenic murine model of systemic amyloidosis. - RESULTS: TME could be successfully performed in control mice below the xiphoid cartilage, with a mean LS of 4.4 ± 1.3 kPa, a mean success rate of 88%, and an excellent intra-observer agreement (0.98). Treatment with CCl4 over seven weeks drastically increased LS as compared to controls (18.2 ± 3.7 kPa vs 3.6 ± 1.2 kPa). Moreover, fibrosis stage was highly correlated with LS (Spearman coefficient = 0.88, P < 0.01). In the amyloidosis model, much higher LS values were obtained, reaching maximum values of > 150 kPa. LS significantly correlated with the amyloidosis index (0.93, P < 0.0001) and the plasma concentration of mutant hapoA-Ⅱ (0.62, P < 0.005). - CONCLUSION: Here, we have established the first non-invasive approach to measure LS in mice, and have successfully validated it in two murine models of high LS.
Item Description:Gesehen am 02.03.2022
Physical Description:Online Resource
ISSN:2219-2840
DOI:10.3748/wjg.v17.i8.968

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