Transient micro-elastography: a novel non-invasive approach to measure liver stiffness in mice
METHODS: A novel transient micro-elastography (TME) device, dedicated to LS measurements in mice with a range of measurement from 1-170 kPa, was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston. The novel probe was validated in a classical fibrosis model (CCl4) and in a t...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
Feb 28, 2011
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| In: |
World journal of gastroenterology
Year: 2011, Jahrgang: 17, Heft: 8, Pages: 968-975 |
| ISSN: | 2219-2840 |
| DOI: | 10.3748/wjg.v17.i8.968 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3748/wjg.v17.i8.968 Verlag, lizenzpflichtig, Volltext: http://www.wjgnet.com/1007-9327/full/v17/i8/968.htm 
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| Verfasserangaben: | Cécile Bastard, Matteo R Bosisio, Michèle Chabert, Athina D Kalopissis, Meriem Mahrouf-Yorgov, Hélène Gilgenkrantz, Sebastian Mueller, Laurent Sandrin |
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| 245 | 1 | 0 | |a Transient micro-elastography |b a novel non-invasive approach to measure liver stiffness in mice |c Cécile Bastard, Matteo R Bosisio, Michèle Chabert, Athina D Kalopissis, Meriem Mahrouf-Yorgov, Hélène Gilgenkrantz, Sebastian Mueller, Laurent Sandrin |
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| 520 | |a METHODS: A novel transient micro-elastography (TME) device, dedicated to LS measurements in mice with a range of measurement from 1-170 kPa, was developed using an optimized vibration frequency of 300 Hz and a 2 mm piston. The novel probe was validated in a classical fibrosis model (CCl4) and in a transgenic murine model of systemic amyloidosis. - RESULTS: TME could be successfully performed in control mice below the xiphoid cartilage, with a mean LS of 4.4 ± 1.3 kPa, a mean success rate of 88%, and an excellent intra-observer agreement (0.98). Treatment with CCl4 over seven weeks drastically increased LS as compared to controls (18.2 ± 3.7 kPa vs 3.6 ± 1.2 kPa). Moreover, fibrosis stage was highly correlated with LS (Spearman coefficient = 0.88, P < 0.01). In the amyloidosis model, much higher LS values were obtained, reaching maximum values of > 150 kPa. LS significantly correlated with the amyloidosis index (0.93, P < 0.0001) and the plasma concentration of mutant hapoA-Ⅱ (0.62, P < 0.005). - CONCLUSION: Here, we have established the first non-invasive approach to measure LS in mice, and have successfully validated it in two murine models of high LS. | ||
| 700 | 1 | |a Bosisio, Matteo R. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Chabert, Michèle |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kalopissis, Athina D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mahrouf-Yorgov, Meriem |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Sandrin, Laurent |e VerfasserIn |4 aut | |
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