Parallelized STED fluorescence nanoscopy
We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, t...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
7 Nov 2011
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| In: |
Optics express
Year: 2011, Volume: 19, Issue: 24, Pages: 23716-23726 |
| ISSN: | 1094-4087 |
| DOI: | 10.1364/OE.19.023716 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1364/OE.19.023716 Verlag, lizenzpflichtig, Volltext: https://opg.optica.org/oe/abstract.cfm?uri=oe-19-24-23716 
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| Author Notes: | Pit Bingen, Matthias Reuss, Johann Engelhardt, and Stefan W. Hell |
| Summary: | We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m. |
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| Item Description: | Gesehen am 24.03.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1094-4087 |
| DOI: | 10.1364/OE.19.023716 |