Antibodies against Tumor Necrosis Factor (TNF) induce t-cell apoptosis in patients with inflammatory bowel diseases via TNF receptor 2 and intestinal CD14+ macrophages
Background & Aims - The anti-tumor necrosis factor (TNF) antibodies infliximab, adalimumab, and certolizumab pegol have proven clinical efficacy in Crohn's disease. Here, we assessed the effects of anti-TNF antibodies on apoptosis in inflammatory bowel disease (IBD). - Methods - CD14+ macro...
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| Hauptverfasser: | , , , , , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
27 August 2011
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| In: |
Gastroenterology
Year: 2011, Jahrgang: 141, Heft: 6, Pages: 2026-2038 |
| ISSN: | 1528-0012 |
| DOI: | 10.1053/j.gastro.2011.08.032 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1053/j.gastro.2011.08.032 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0016508511012157 
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| Verfasserangaben: | Raja Atreya, Michael Zimmer, Brigitte Bartsch, Maximilian J. Waldner, Imke Atreya, Helmut Neumann, Kai Hildner, Arthur Hoffman, Ralf Kiesslich, Andreas D. Rink, Tilman T. Rau, Stefan Rose-John, Hermann Kessler, Jan Schmidt, and Markus F. Neurath |
MARC
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| 245 | 1 | 0 | |a Antibodies against Tumor Necrosis Factor (TNF) induce t-cell apoptosis in patients with inflammatory bowel diseases via TNF receptor 2 and intestinal CD14+ macrophages |c Raja Atreya, Michael Zimmer, Brigitte Bartsch, Maximilian J. Waldner, Imke Atreya, Helmut Neumann, Kai Hildner, Arthur Hoffman, Ralf Kiesslich, Andreas D. Rink, Tilman T. Rau, Stefan Rose-John, Hermann Kessler, Jan Schmidt, and Markus F. Neurath |
| 246 | 3 | 3 | |a Antibodies against Tumor Necrosis Factor (TNF) induce t-cell apoptosis in patients with inflammatory bowel diseases via TNF receptor 2 and intestinal CD14 + macrophages |
| 264 | 1 | |c 27 August 2011 | |
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| 520 | |a Background & Aims - The anti-tumor necrosis factor (TNF) antibodies infliximab, adalimumab, and certolizumab pegol have proven clinical efficacy in Crohn's disease. Here, we assessed the effects of anti-TNF antibodies on apoptosis in inflammatory bowel disease (IBD). - Methods - CD14+ macrophages and CD4+ T cells were isolated from peripheral blood and lamina propria mononuclear cells from patients with IBD and control patients. Cell surface markers and apoptosis were assessed by immunohistology and fluorescence-activated cell sorting techniques. - Results - Lamina propria CD14+ macrophages showed significantly more frequent and higher membrane-bound TNF (mTNF) expression than CD4+ T cells in IBD, whereas mTNF-dependent signaling proteins such as TNF receptor (TNFR) 2, TNFR-associated factor (TRAF) 2, and nuclear factor κB were induced in IBD mucosal CD4+ T cells. Most anti-TNF antibodies did not induce T-cell apoptosis in purified peripheral or mucosal CD4+ T cells. However, in contrast to etanercept, administration of all clinically effective anti-TNF antibodies resulted in a significant induction of T-cell apoptosis in IBD when lamina propria CD4+ T cells expressing TNFR2+ were cocultured with mTNF+ CD14+ intestinal macrophages. In contrast, no effects in control patients were noted. T-cell apoptosis in IBD occurred in vivo after treatment with adalimumab and infliximab, was critically dependent on TNFR2 signaling, and could be prevented via interleukin-6 signal transduction. Blockade of interleukin-6R signaling augmented anti-TNF-induced T-cell apoptosis in IBD. - Conclusions - Clinically effective anti-TNF antibodies are able to induce T-cell apoptosis in IBD only when mucosal TNFR2+ T cells are cocultured with mTNF-expressing CD14+ macrophages. The finding that anti-TNF antibodies induce apoptosis indirectly by targeting the mTNF/TNFR2 pathway may have important implications for the development of new therapeutic strategies in IBD. | ||
| 650 | 4 | |a Apoptosis Induction | |
| 650 | 4 | |a Crohn's Disease | |
| 650 | 4 | |a IL-6 | |
| 650 | 4 | |a mTNF | |
| 700 | 1 | |a Zimmer, Michael |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bartsch, Brigitte |e VerfasserIn |4 aut | |
| 700 | 1 | |a Waldner, Maximilian J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Atreya, Imke |e VerfasserIn |4 aut | |
| 700 | 1 | |a Neumann, Helmut |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hildner, Kai |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hoffman, Arthur |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kiesslich, Ralf |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rink, Andreas D. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rau, Tilman T. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rose-John, Stefan |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Neurath, Markus F. |e VerfasserIn |4 aut | |
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