Targeting TGF-β1 increases hepatocyte growth factor (HGF/SF) levels in external auditory canal cholesteatoma (EACC) epithelial cell culture
Objective - The transforming growth factor (TGF)-β 1 is known to have pro- and anti-angiogenic actions. Hepatocyte growth factor/scatter factor (HGF/SF) antagonizes TGF-β1 by stabilizing SMAD transcriptional co-repressor TGIF. HGF/SF is a multifunctional polypeptide with morphogenic, motogenic, angi...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 August 2005
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| In: |
Regulatory peptides
Year: 2005, Jahrgang: 130, Heft: 1, Pages: 75-80 |
| ISSN: | 1873-1686 |
| DOI: | 10.1016/j.regpep.2005.03.008 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.regpep.2005.03.008 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0167011505001151 
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| Verfasserangaben: | Ramin Naim, Ray C. Chang, Suzanne S. Alfano, Frank Riedel, Christiane Bayerl, Haneen Sadick, Gregor Bran, Karl Hormann |
| Zusammenfassung: | Objective - The transforming growth factor (TGF)-β 1 is known to have pro- and anti-angiogenic actions. Hepatocyte growth factor/scatter factor (HGF/SF) antagonizes TGF-β1 by stabilizing SMAD transcriptional co-repressor TGIF. HGF/SF is a multifunctional polypeptide with morphogenic, motogenic, angiogenic, and proliferative capabilities. We assume HGF to be a pivotal factor during the pathogenesis of the external auditory canal cholesteatoma (EACC). In this study, we investigate the effect of antisense targeting TGF-β1 on HGF/SF levels in the epithelial EACC-culture. - Materials - For 48 h, epithelial EACC cell culture was incubated with 3 and 6 μmol antisense targeting TGF-β1, respectively. Levels of HGF/SF were determined and normalized to cellular protein. Untreated EACC cell culture and scrambled TGF-β1-antisense served as control. In the second experiment, EACC cells were incubated with rh TGF-β1 (2 and 4 ng/ml) for 48 h and HGF/SF was determined. - Results - After incubation with 3 μmol TGF-β1-antisense, the average level of HGF/SF was measured at 43.68 pg/ml. Incubation with 6 μmol TGF-β1-antisense showed 64.95 pg/ml. In untreated EACC (control), the average level of HGF/SF after 48 was 34.55 pg/ml. Incubation with scrambled TGF-β1 oligonucleotide showed an average HGF/SF level of 34.41 (3 μmol) and 35.66 (6 μmol), respectively. The difference between the scrambled antisense and the targeting antisense TGF-β1 was significant (p<0.05). After incubation with 2 ng/ml TGF-β1, the HGF/SF levels were at 22.16 pg/ml. TGF-β1, 4 ng/ml, resulted in 15.33 pg/ml of HGF/SF. The difference of the levels of HGF/SF after incubation with exogenous TGF-β1 was significant (p<0.05). - Conclusion - In this study, levels of HGF/SF increased in the epithelial EACC cell culture after incubation with 3 and 6 μmol antisense TGF-β1 oligonucleotides depending on the concentration of the antisense. In reverse, TGF-β1 acted as inhibiting cytokine on HGF/SF levels. In conclusion, TGF-β1 may be a useful therapeutic agent for managing EACC. |
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| Beschreibung: | Available online: 23 May 2005 Gesehen am 25.03.2022 |
| Beschreibung: | Online Resource |
| ISSN: | 1873-1686 |
| DOI: | 10.1016/j.regpep.2005.03.008 |