Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis
Background/Aims:Elevated concentrations of tumor necrosis factor receptors have been detected in alcoholic cirrhosis, but it remains unknown whether or not peripheral blood mononuclear cells are a source of tumor necrosis factor receptors and reflect the clinical disease activity of patients with ad...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2000
|
| In: |
Journal of hepatology
Year: 2000, Jahrgang: 32, Heft: 1, Pages: 51-57 |
| ISSN: | 1600-0641 |
| DOI: | 10.1016/S0168-8278(00)80189-0 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0168-8278(00)80189-0 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0168827800801890 
|
| Verfasserangaben: | Christoph Hanck, Michael Glatzel, Manfred Vincenz Singer and Siegbert Rossol |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1796687502 | ||
| 003 | DE-627 | ||
| 005 | 20230428010635.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220325s2000 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/S0168-8278(00)80189-0 |2 doi | |
| 035 | |a (DE-627)1796687502 | ||
| 035 | |a (DE-599)KXP1796687502 | ||
| 035 | |a (OCoLC)1341458032 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hanck, Christoph |e VerfasserIn |0 (DE-588)1254060928 |0 (DE-627)1796679534 |4 aut | |
| 245 | 1 | 0 | |a Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis |c Christoph Hanck, Michael Glatzel, Manfred Vincenz Singer and Siegbert Rossol |
| 264 | 1 | |c 2000 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Available online 4 June 2001 | ||
| 500 | |a Gesehen am 25.03.2022 | ||
| 520 | |a Background/Aims:Elevated concentrations of tumor necrosis factor receptors have been detected in alcoholic cirrhosis, but it remains unknown whether or not peripheral blood mononuclear cells are a source of tumor necrosis factor receptors and reflect the clinical disease activity of patients with advanced alcoholic liver disease. Methods: Twenty-two abstinent patients in different stages of alcohol-induced cirrhosis according to the criteria of the Child-Pugh classification (Child-Pugh stage A: 4, Child-Pugh stage B: 10, Child-Pugh stage C: 8) were compared with four healthy individuals. Semi-quantitative reverse transcriptase-polymerase chain reaction was used for the measurement of the expression of tumor necrosis factor-α, soluble tumor necrosis factor receptors-p55, -p75, interleukin-10 and inducible nitric oxide synthase in unstimulated peripheral blood mononuclear cells. Results: Unstimulated peripheral blood mononuclear cells of patients with alcoholic cirrhosis demonstrate a stage-dependent enhanced RNA expression of tumor necrosis factor-α (healthy controls 0/4, Child-Pugh stage A 2/4, stage B 10/10, stage C 8/8; p<0.01). The mRNA expression of TNF-receptors-p55/-p75 is significantly higher in patients with severe alcoholic cirrhosis (Child-Pugh stage B or C patients) than healthy controls (p<0.05), while peripheral blood mononuclear cells from patients with Child-Pugh stage A show a similiar pattern of gene expression to healthy controls. No significant upregulation of interleukin-10 was found. Inducible nitric oxide synthase was detectable in Child-Pugh stage C (p<0.05). Conclusions: Unstimulated peripheral blood mononuclear cells of patients with severe alcoholic cirrhosis (Child-Pugh stage B and C) demonstrate a systemic leukocyte activation and gene expression of tumor necrosis factor-α and tumor necrosis factor receptors-p55/-p75, which is correlated with the activity of the disease. Our data confirm previous studies that reported a correlation between plasma levels of pro-inflammatory cytokines and the severity of alcoholic cirrhosis. The role of interleukin-10 and inducible nitric oxide synthase in the pathogenesis of alcoholic cirrhosis remains to be fully elucidated. | ||
| 650 | 4 | |a Alcoholic cirrhosis | |
| 650 | 4 | |a Cytokine gene expression | |
| 650 | 4 | |a Soluble tumor necrosis factor receptors | |
| 650 | 4 | |a Tumor necrosis factor-α | |
| 650 | 4 | |a Unstimulated peripheral blood mononuclear cells | |
| 700 | 1 | |a Glatzel, Michael |d 1972- |e VerfasserIn |0 (DE-588)122390008 |0 (DE-627)705890597 |0 (DE-576)29324989X |4 aut | |
| 700 | 1 | |a Singer, Manfred V. |d 1945- |e VerfasserIn |0 (DE-588)121081958 |0 (DE-627)081074069 |0 (DE-576)169905330 |4 aut | |
| 700 | 1 | |a Rossol, Siegbert |e VerfasserIn |0 (DE-588)1106566408 |0 (DE-627)863173241 |0 (DE-576)179083627 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of hepatology |d Amsterdam [u.a.] : Elsevier Science, 1985 |g 32(2000), 1 vom: Jan., Seite 51-57 |h Online-Ressource |w (DE-627)320984486 |w (DE-600)2027112-8 |w (DE-576)093888856 |x 1600-0641 |7 nnas |a Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis |
| 773 | 1 | 8 | |g volume:32 |g year:2000 |g number:1 |g month:01 |g pages:51-57 |g extent:7 |a Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis |
| 856 | 4 | 0 | |u https://doi.org/10.1016/S0168-8278(00)80189-0 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0168827800801890 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220325 | ||
| 993 | |a Article | ||
| 994 | |a 2000 | ||
| 998 | |g 1106566408 |a Rossol, Siegbert |m 1106566408:Rossol, Siegbert |d 60000 |d 61100 |e 60000PR1106566408 |e 61100PR1106566408 |k 0/60000/ |k 1/60000/61100/ |p 4 |y j | ||
| 998 | |g 121081958 |a Singer, Manfred V. |m 121081958:Singer, Manfred V. |d 60000 |d 61100 |e 60000PS121081958 |e 61100PS121081958 |k 0/60000/ |k 1/60000/61100/ |p 3 | ||
| 998 | |g 1254060928 |a Hanck, Christoph |m 1254060928:Hanck, Christoph |d 60000 |d 61100 |e 60000PH1254060928 |e 61100PH1254060928 |k 0/60000/ |k 1/60000/61100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1796687502 |e 4100510195 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis","title":"Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosis"}],"physDesc":[{"extent":"7 S."}],"name":{"displayForm":["Christoph Hanck, Michael Glatzel, Manfred Vincenz Singer and Siegbert Rossol"]},"id":{"doi":["10.1016/S0168-8278(00)80189-0"],"eki":["1796687502"]},"origin":[{"dateIssuedDisp":"2000","dateIssuedKey":"2000"}],"relHost":[{"id":{"issn":["1600-0641"],"zdb":["2027112-8"],"eki":["320984486"]},"part":{"volume":"32","issue":"1","text":"32(2000), 1 vom: Jan., Seite 51-57","year":"2000","extent":"7","pages":"51-57"},"recId":"320984486","note":["Gesehen am 18.02.2020"],"pubHistory":["1.1985 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"subtitle":"official journal of the European Association for the Study of the Liver","title":"Journal of hepatology","title_sort":"Journal of hepatology"}],"origin":[{"dateIssuedKey":"1985","publisherPlace":"Amsterdam [u.a.] ; [Erscheinungsort nicht ermittelbar]","publisher":"Elsevier Science ; Wiley-Blackwell","dateIssuedDisp":"1985-"}],"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"disp":"Gene expression of TNF-receptors in peripheral blood mononuclear cells of patients with alcoholic cirrhosisJournal of hepatology"}],"person":[{"display":"Hanck, Christoph","given":"Christoph","role":"aut","roleDisplay":"VerfasserIn","family":"Hanck"},{"given":"Michael","roleDisplay":"VerfasserIn","role":"aut","display":"Glatzel, Michael","family":"Glatzel"},{"display":"Singer, Manfred V.","roleDisplay":"VerfasserIn","role":"aut","given":"Manfred V.","family":"Singer"},{"display":"Rossol, Siegbert","roleDisplay":"VerfasserIn","role":"aut","given":"Siegbert","family":"Rossol"}],"language":["eng"],"note":["Available online 4 June 2001","Gesehen am 25.03.2022"],"recId":"1796687502","type":{"bibl":"article-journal","media":"Online-Ressource"}} | ||
| SRT | |a HANCKCHRISGENEEXPRES2000 | ||