Biliary obstruction exacerbates the hepatic microvascular inflammatory response to endotoxin
Gram-negative sepsis is a serious complication for patients with obstructive jaundice. The present study was conducted to elucidate the response of hepatic microcirculation to endotoxin 2 weeks after bile duct ligation (BDL) or sham-operation in rats. Two hours after lipopolysaccharide (LPS) injecti...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 2000
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| In: |
Shock
Year: 2000, Volume: 14, Issue: 6, Pages: 599-604 |
| ISSN: | 1540-0514 |
| DOI: | 10.1097/00024382-200014060-00005 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1097/00024382-200014060-00005 Verlag, lizenzpflichtig, Volltext: https://journals.lww.com/shockjournal/Abstract/2000/14060/BILIARY_OBSTRUCTION_EXACERBATES_THE_HEPATIC.5.aspx 
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| Author Notes: | Yoshiya Ito, Nancy W. Machen, Renate Urbaschek, and Robert S. McCuskey |
| Summary: | Gram-negative sepsis is a serious complication for patients with obstructive jaundice. The present study was conducted to elucidate the response of hepatic microcirculation to endotoxin 2 weeks after bile duct ligation (BDL) or sham-operation in rats. Two hours after lipopolysaccharide (LPS) injection (1,10, or 100 μg/kg, iv.), the hepatic microvasculature was examined using in vivo microscopy. BDL elicited increases in leukocytes adhering to the sinusoidal wall, swelling of sinusoidal endothelial cells as well as phagocytic activity of hepatic macrophages and a decrease in the numbers of perfused sinusoids. LPS (1, 10, 100 μg/kg) further increased leukocyte adhesion and reduced the numbers of perfused sinusoids in a dose-dependent manner. Leukocyte adhesion in response to LPS (1, 10, 100 μg/kg) in BDL rats was increased 6.1-fold, 5.9-fold, and 3.3-fold, respectively when compared with sham-operated rats. The numbers of perfused sinusoids in response to LPS (1, 10, 100 μg/kg) in BDL rats were decreased by 42%, 36%, and 45%. While 1 and 10 μg/kg LPS also elicited an increase in phagocytic activity in BDL rats when compared with sham-operated rats, the response to 100 μg/kg LPS was suppressed. LPS did not affect the numbers of swollen endothelial cell in BDL rats. The present study demonstrated that chronic biliary obstruction enhanced the hepatic microvascular response to low doses of endotoxin. This observation suggests that exaggerated hepatic microcirculatory dysfunction during sepsis contributes to the development of liver injury and a high incidence of morbidity and mortality in biliary obstruction. |
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| Item Description: | Gesehen am 29.03.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1540-0514 |
| DOI: | 10.1097/00024382-200014060-00005 |