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Subcellular identification of angiotensin I/II- and angiotensin II (AT1)-receptor-immunoreactivity in the central nervous system of rats

To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT1-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT1-receptor-immunolabelling at brain parenchyma ve...

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Bibliographic Details
Main Authors: Thomas, Martin Alexander (Author) , Fleissner, Gerta (Author) , Hauptfleisch, Stefan (Author) , Lemmer, Björn (Author)
Format: Article (Journal)
Language:English
Published: 2003
In: Brain research
Year: 2003, Volume: 962, Issue: 1/2, Pages: 92-104
ISSN:1872-6240
DOI:10.1016/S0006-8993(02)03971-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0006-8993(02)03971-9
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006899302039719
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Author Notes:Martin Alexander Thomas, Gerta Fleissner, Stefan Hauptfleisch, Björn Lemmer
Description
Summary:To gain insight into generating and transport mechanisms of angiotensin (ANG) in the brain the study was focused on the subcellular localization of ANG II and its AT1-receptors in the hypothalamus of rats. The present paper demonstrates ANG II- and AT1-receptor-immunolabelling at brain parenchyma vessels and at glial and neuronal structures in the perivascular region. Further, ANG II- and AT1-receptor-immunoreactivity is shown at plasma membranes and intracellular structures in the ependyma of the third ventricle. Based upon a conventional horseradish peroxidase technique, combined with the classical substrate 3,3′-diaminobenzidine, a procedure is introduced that will be useful with a variety of antibodies used on glutar- and paraformaldehyde-fixed brain tissue. This technique enables a fast correlation between light and electron microscopical results and might also provide an attractive alternative to colloidal gold-labelling and silver-intensification techniques.
Item Description:Available online 17 December 2002
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Physical Description:Online Resource
ISSN:1872-6240
DOI:10.1016/S0006-8993(02)03971-9

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