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Absence of neocytolysis in humans returning from a 3-week high-altitude sojourn

Aims Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a pro...

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Main Authors: Klein, Marie (Author) , Kaestner, Lars (Author) , Bogdanova, Anna Y. (Author) , Minetti, Giampaolo (Author) , Rudloff, Silvia (Author) , Lundby, Carsten (Author) , Makhro, Asya (Author) , Seiler, Elena (Author) , van Cromvoirt, Ankie (Author) , Fenk, Simone (Author) , Simionato, Greta (Author) , Hertz, Laura (Author) , Recktenwald, Steffen (Author) , Schäfer, Larissa (Author) , Haider, Thomas (Author) , Fried, Sebastian (Author) , Borsch, Christian (Author) , Marti, Hugo (Author) , Sander, Anja (Author) , Mairbäurl, Heimo (Author)
Format: Article (Journal)
Language:English
Published: 17 March 2021
In: Acta physiologica
Year: 2021, Volume: 232, Pages: 1-11
ISSN:1748-1716
DOI:10.1111/apha.13647
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1111/apha.13647
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.13647
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Author Notes:Marie Klein, Lars Kaestner, Anna Y. Bogdanova, Giampaolo Minetti, Silvia Rudloff, Carsten Lundby, Asya Makhro, Elena Seiler, Ankie van Cromvoirt, Simone Fenk, Greta Simionato, Laura Hertz, Steffen Recktenwald, Larissa Schäfer, Thomas Haider, Sebastian Fried, Christian Borsch, Hugo H. Marti, Anja Sander, Heimo Mairbäurl
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Summary:Aims Total haemoglobin mass (tot-Hb) increases during high-altitude acclimatization. Normalization of tot-Hb upon descent is thought to occur via neocytolysis, the selective destruction of newly formed erythrocytes. Because convincing experimental proof of neocytolysis is lacking, we performed a prospective study on erythrocyte survival after a stay at the Jungfraujoch Research Station (JFJRS; 3450 m). Methods Newly formed erythrocytes of 12 male subjects (mean age 23.3 years) were age cohort labelled in normoxia (110 m) and during a 19-day high-altitude sojourn by ingestion of 13C2- and 15N-labelled glycine respectively. Elimination dynamics for erythrocytes produced in normoxia and at high altitude were measured by isotope ratio mass spectrometry of haem, by determining tot-Hb, reticulocyte counts, erythrocyte membrane protein 4.1a/4.1b ratio and by mathematical modelling. Results Tot-Hb increased by 4.7% ± 2.7% at high altitude and returned to pre-altitude values within 11 days after descent. Elimination of 13C- (normoxia) and 15N- (high altitude) labelled erythrocytes was not different. Erythropoietin levels and counts of CD71-positive reticulocytes decreased rapidly after descent. The band 4.1a/4.1b ratio decreased at altitude and remained low for 3-4 days after descent and normalized slowly. There was no indication of haemolysis. Conclusion We confirm a rapid normalization of tot-Hb upon descent. Based on the lack of accelerated removal of age cohorts of erythrocytes labelled at high altitude, on patterns of changes in reticulocyte counts and of the band 4.1a/4.1b ratio and on modelling, this decrease did not occur via neocytolysis, but by a reduced rate of erythropoiesis along with normal clearance of senescent erythrocytes.
Item Description:Gesehen am 07.04.2022
Physical Description:Online Resource
ISSN:1748-1716
DOI:10.1111/apha.13647

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