Evaluation of serum-free culture conditions for primary human nasal epithelial cells
Defined culture conditions are essential for the interpretation of effects caused by volatile substances on human nasal epithelial cells (HUNEC) cultured in vitro. Conventionally, serum-containing media are used. However, the results of these experiments are of restricted value as serum contains man...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
[2002]
|
| In: |
International journal of hygiene and environmental health
Year: 2002, Volume: 205, Issue: 3, Pages: 235-238 |
| ISSN: | 1618-131X |
| DOI: | 10.1078/1438-4639-00118 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1078/1438-4639-00118 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1438463904701509 
|
| Author Notes: | Cathrine Mattinger, Trang Nyugen, Dirk Schäfer, Karl Hörmann (Department of Otorhinolaryngology, Kompetenzzentrum Umweltmedizin, University Hospital Mannheim) |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1800401736 | ||
| 003 | DE-627 | ||
| 005 | 20220820172302.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220427s2002 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1078/1438-4639-00118 |2 doi | |
| 035 | |a (DE-627)1800401736 | ||
| 035 | |a (DE-599)KXP1800401736 | ||
| 035 | |a (OCoLC)1341459217 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Mattinger, Cathrine |e VerfasserIn |0 (DE-588)1256319481 |0 (DE-627)1800401957 |4 aut | |
| 245 | 1 | 0 | |a Evaluation of serum-free culture conditions for primary human nasal epithelial cells |c Cathrine Mattinger, Trang Nyugen, Dirk Schäfer, Karl Hörmann (Department of Otorhinolaryngology, Kompetenzzentrum Umweltmedizin, University Hospital Mannheim) |
| 264 | 1 | |c [2002] | |
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 27.04.2022 | ||
| 500 | |a Available online 14 November 2004 | ||
| 520 | |a Defined culture conditions are essential for the interpretation of effects caused by volatile substances on human nasal epithelial cells (HUNEC) cultured in vitro. Conventionally, serum-containing media are used. However, the results of these experiments are of restricted value as serum contains many unknown and undefined substances. Not all serum-free media on the market are suitable for culturing primary HUNEC. Therefore, serum-free defined cell culture media were compared to evaluate optimal conditions for HUNEC and their cell lines. HUNEC were generated by trypsin digestion of mucosal tissue of the inferior turbinate. Cells were cultured on uncoated polystyrene dishes adding pre-warmed medium. Viability was controlled by trypan blue dye exclusion; colony forming units and cell morphology were controlled microscopically. The expression of different cytokeratins was studied immunocytochemically. Dulbecco's modified Eagle's medium was not suitable to grow HUNEC and passage them. HUNEC cultured in bronchial epithelial growth medium presented a more homogeneous cell morphology compared to other media and had a doubling time of 1.2 days. The maximum number of cell passages was 11 with bronchial epithelial growth medium. | ||
| 650 | 4 | |a cell culture | |
| 650 | 4 | |a Human nasal epithelial cell | |
| 650 | 4 | |a serum-free medium | |
| 700 | 1 | |a Nguyen, Trang |e VerfasserIn |0 (DE-588)1256319880 |0 (DE-627)1800402333 |4 aut | |
| 700 | 1 | |a Schäfer, Dirk |e VerfasserIn |0 (DE-588)1256320374 |0 (DE-627)1800403208 |4 aut | |
| 700 | 1 | |a Hörmann, Karl |d 1948- |e VerfasserIn |0 (DE-588)130123749 |0 (DE-627)49171193X |0 (DE-576)266607438 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of hygiene and environmental health |d München : Elsevier, 2000 |g 205(2002), 3, Seite 235-238 |h Online-Ressource |w (DE-627)325789576 |w (DE-600)2039765-3 |w (DE-576)09592566X |x 1618-131X |7 nnas |a Evaluation of serum-free culture conditions for primary human nasal epithelial cells |
| 773 | 1 | 8 | |g volume:205 |g year:2002 |g number:3 |g pages:235-238 |g extent:4 |a Evaluation of serum-free culture conditions for primary human nasal epithelial cells |
| 856 | 4 | 0 | |u https://doi.org/10.1078/1438-4639-00118 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S1438463904701509 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20220427 | ||
| 993 | |a Article | ||
| 994 | |a 2002 | ||
| 998 | |g 130123749 |a Hörmann, Karl |m 130123749:Hörmann, Karl |d 60000 |d 62100 |e 60000PH130123749 |e 62100PH130123749 |k 0/60000/ |k 1/60000/62100/ |p 4 |y j | ||
| 998 | |g 1256320374 |a Schäfer, Dirk |m 1256320374:Schäfer, Dirk |d 60000 |d 62100 |e 60000PS1256320374 |e 62100PS1256320374 |k 0/60000/ |k 1/60000/62100/ |p 3 | ||
| 998 | |g 1256319880 |a Nguyen, Trang |m 1256319880:Nguyen, Trang |d 60000 |d 62100 |e 60000PN1256319880 |e 62100PN1256319880 |k 0/60000/ |k 1/60000/62100/ |p 2 | ||
| 998 | |g 1256319481 |a Mattinger, Cathrine |m 1256319481:Mattinger, Cathrine |d 60000 |d 62100 |e 60000PM1256319481 |e 62100PM1256319481 |k 0/60000/ |k 1/60000/62100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1800401736 |e 4124544324 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 27.04.2022","Available online 14 November 2004"],"relHost":[{"note":["Gesehen am 13.02.20"],"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1618-131X"],"zdb":["2039765-3"],"eki":["325789576"]},"part":{"extent":"4","issue":"3","year":"2002","pages":"235-238","text":"205(2002), 3, Seite 235-238","volume":"205"},"title":[{"title":"International journal of hygiene and environmental health","subtitle":"official journal of the German Society of Hygiene, Environmental and Public Health Sciences (Gesellschaft für Hygiene, Umweltmedizin und Präventivmedizin, GHUP)","title_sort":"International journal of hygiene and environmental health"}],"origin":[{"dateIssuedDisp":"2000-","publisher":"Elsevier ; Urban & Fischer ; Urban & Fischer","publisherPlace":"München ; Jena ; Jena","dateIssuedKey":"2000"}],"corporate":[{"role":"isb","display":"Gesellschaft für Hygiene, Umweltmedizin und Präventivmedizin"},{"display":"Deutsche Gesellschaft für Hygiene und Umweltmedizin","role":"isb"}],"pubHistory":["203.2000 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"titleAlt":[{"title":"official journal of the German Society for Hygiene and Environmental Medicine (Gesellschaft für Hygiene und Umweltmedizin, GHU)"}],"recId":"325789576","disp":"Evaluation of serum-free culture conditions for primary human nasal epithelial cellsInternational journal of hygiene and environmental health"}],"physDesc":[{"extent":"4 S."}],"id":{"eki":["1800401736"],"doi":["10.1078/1438-4639-00118"]},"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Evaluation of serum-free culture conditions for primary human nasal epithelial cells","title":"Evaluation of serum-free culture conditions for primary human nasal epithelial cells"}],"language":["eng"],"person":[{"family":"Mattinger","given":"Cathrine","display":"Mattinger, Cathrine","role":"aut"},{"family":"Nguyen","given":"Trang","display":"Nguyen, Trang","role":"aut"},{"role":"aut","family":"Schäfer","given":"Dirk","display":"Schäfer, Dirk"},{"role":"aut","family":"Hörmann","display":"Hörmann, Karl","given":"Karl"}],"name":{"displayForm":["Cathrine Mattinger, Trang Nyugen, Dirk Schäfer, Karl Hörmann (Department of Otorhinolaryngology, Kompetenzzentrum Umweltmedizin, University Hospital Mannheim)"]},"origin":[{"dateIssuedKey":"2002","dateIssuedDisp":"[2002]"}],"recId":"1800401736"} | ||
| SRT | |a MATTINGERCEVALUATION2002 | ||