Buchumschlag

TaqMan-based quantification of invasive cells in the chick embryo metastasis assay

The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan® technology to quantify the invaded...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Htun van der Horst, Edward (VerfasserIn) , Leupold, Jörg (VerfasserIn) , Schubbert, Rainer (VerfasserIn) , Ullrich, Axel (VerfasserIn) , Allgayer, Heike (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2004
In: BioTechniques
Year: 2004, Jahrgang: 37, Heft: 6, Pages: 940-946
ISSN:1940-9818
DOI:10.2144/04376ST02
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2144/04376ST02
Verlag, lizenzpflichtig, Volltext: https://www.future-science.com/doi/10.2144/04376ST02
Volltext
Verfasserangaben:Edward Htun van der Horst, Jörg H. Leupold, Rainer Schubbert, Axel Ullrich, and Heike Allgayer

MARC

LEADER 00000caa a2200000 c 4500
001 1800634277
003 DE-627
005 20220820173455.0
007 cr uuu---uuuuu
008 220502s2004 xx |||||o 00| ||eng c
024 7 |a 10.2144/04376ST02  |2 doi 
035 |a (DE-627)1800634277 
035 |a (DE-599)KXP1800634277 
035 |a (OCoLC)1341459472 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Htun van der Horst, Edward  |d 1973-  |e VerfasserIn  |0 (DE-588)124262104  |0 (DE-627)085745294  |0 (DE-576)294092552  |4 aut 
245 1 0 |a TaqMan-based quantification of invasive cells in the chick embryo metastasis assay  |c Edward Htun van der Horst, Jörg H. Leupold, Rainer Schubbert, Axel Ullrich, and Heike Allgayer 
264 1 |c 2004 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Elektronische Reproduktion der Druck-Ausgabe 
500 |a Published online: 6 Jun 2018 
500 |a Gesehen am 02.05.2022 
520 |a The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan® technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range. 
700 1 |a Leupold, Jörg  |d 1969-  |e VerfasserIn  |0 (DE-588)130056030  |0 (DE-627)488795796  |0 (DE-576)29797310X  |4 aut 
700 1 |a Schubbert, Rainer  |e VerfasserIn  |4 aut 
700 1 |a Ullrich, Axel  |e VerfasserIn  |4 aut 
700 1 |a Allgayer, Heike  |d 1969-  |e VerfasserIn  |0 (DE-588)1024987884  |0 (DE-627)720854466  |0 (DE-576)369805003  |4 aut 
773 0 8 |i Enthalten in  |t BioTechniques  |d London, UK : Future Science Ltd, 1983  |g 37(2004), 6, Seite 940-946  |h Online-Ressource  |w (DE-627)306320746  |w (DE-600)1496354-1  |w (DE-576)094115559  |x 1940-9818  |7 nnas  |a TaqMan-based quantification of invasive cells in the chick embryo metastasis assay 
773 1 8 |g volume:37  |g year:2004  |g number:6  |g pages:940-946  |g extent:7  |a TaqMan-based quantification of invasive cells in the chick embryo metastasis assay 
856 4 0 |u https://doi.org/10.2144/04376ST02  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.future-science.com/doi/10.2144/04376ST02  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20220502 
993 |a Article 
994 |a 2004 
998 |g 1024987884  |a Allgayer, Heike  |m 1024987884:Allgayer, Heike  |p 5  |y j 
998 |g 130056030  |a Leupold, Jörg  |m 130056030:Leupold, Jörg  |d 60000  |d 61800  |e 60000PL130056030  |e 61800PL130056030  |k 0/60000/  |k 1/60000/61800/  |p 2 
999 |a KXP-PPN1800634277  |e 4128050814 
BIB |a Y 
SER |a journal 
JSO |a {"name":{"displayForm":["Edward Htun van der Horst, Jörg H. Leupold, Rainer Schubbert, Axel Ullrich, and Heike Allgayer"]},"id":{"eki":["1800634277"],"doi":["10.2144/04376ST02"]},"origin":[{"dateIssuedDisp":"2004","dateIssuedKey":"2004"}],"relHost":[{"recId":"306320746","language":["eng"],"note":["Gesehen am 30.05.2024","Frühere Jahrgänge online nicht mehr verfügbar"],"disp":"TaqMan-based quantification of invasive cells in the chick embryo metastasis assayBioTechniques","type":{"media":"Online-Ressource","bibl":"periodical"},"part":{"extent":"7","volume":"37","text":"37(2004), 6, Seite 940-946","issue":"6","pages":"940-946","year":"2004"},"pubHistory":["1.1983 -"],"title":[{"title_sort":"BioTechniques","title":"BioTechniques","subtitle":"the international journal of life science methods"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1940-9818"],"zdb":["1496354-1"],"eki":["306320746"]},"origin":[{"publisher":"Future Science Ltd ; Eaton Publ. Co. ; Eaton ; Informa Life Sciences Publ.","dateIssuedKey":"1983","dateIssuedDisp":"1983-","publisherPlace":"London, UK ; Natick, Mass. ; Westborough, Mass. ; Westborough, Mass."}]}],"physDesc":[{"extent":"7 S."}],"person":[{"role":"aut","roleDisplay":"VerfasserIn","display":"Htun van der Horst, Edward","given":"Edward","family":"Htun van der Horst"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Leupold, Jörg","given":"Jörg","family":"Leupold"},{"given":"Rainer","family":"Schubbert","role":"aut","display":"Schubbert, Rainer","roleDisplay":"VerfasserIn"},{"display":"Ullrich, Axel","roleDisplay":"VerfasserIn","role":"aut","family":"Ullrich","given":"Axel"},{"family":"Allgayer","given":"Heike","roleDisplay":"VerfasserIn","display":"Allgayer, Heike","role":"aut"}],"title":[{"title":"TaqMan-based quantification of invasive cells in the chick embryo metastasis assay","title_sort":"TaqMan-based quantification of invasive cells in the chick embryo metastasis assay"}],"language":["eng"],"recId":"1800634277","note":["Elektronische Reproduktion der Druck-Ausgabe","Published online: 6 Jun 2018","Gesehen am 02.05.2022"],"type":{"media":"Online-Ressource","bibl":"article-journal"}} 
SRT |a HTUNVANDERTAQMANBASE2004 

Ähnliche Einträge